中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
38期
3005-3007
,共3页
马天加%萧畔%徐洋%刘玉强%李玉瑭%史培堃%周亚滨
馬天加%蕭畔%徐洋%劉玉彊%李玉瑭%史培堃%週亞濱
마천가%소반%서양%류옥강%리옥당%사배곤%주아빈
膀胱肿瘤%肿瘤标记,生物学%骨桥蛋白质%CIP2A
膀胱腫瘤%腫瘤標記,生物學%骨橋蛋白質%CIP2A
방광종류%종류표기,생물학%골교단백질%CIP2A
Urinary bladder neoplasms%Tumor markers,biological%Osteopontin%Cancerous inhibitor of PP2A
目的 探讨蛋白磷酸酶2A的癌性抑制因子(CIP2A)、骨桥蛋白(OPN)在膀胱癌中的表达及其在膀胱癌发生发展中的作用.方法 对2010年5月至2012年12月山东大学第二医院泌尿外科收治的膀胱癌患者,采用反转录PCR (RT-PCR)检测38例患者肿瘤组织和12例患者癌旁正常膀胱组织中CIP2A mRNA、OPN mRNA的表达;免疫组化染色法检测99例患者肿瘤组织和12例患者癌旁正常膀胱组织中CIP2A、OPN蛋白的表达.结果 RT-PCR显示38例膀胱癌患者中29例(76.32％)检测到CIP2A mRNA的表达,35例(92.11％)检测到OPN mRNA的表达,正常膀胱组织中二者均未有表达(均P＜0.05).免疫组化结果显示99例膀胱癌患者中有63例(63.64％) CIP2A蛋白阳性,84例(84.85％)OPN蛋白阳性;而正常膀胱组织中未检测到CIP2A蛋白,2例OPN蛋白阳性(均P＜0.05).99例膀胱癌患者中,CIP2A蛋白与OPN蛋白表达两者均阳性的58例(58.59％),两者均阴性的13例,CIP2A阳性而OPN阴性8例,CIP2A阴性而OPN阳性20例(r=0.300,P＜0.05).结论 CIP2A、OPN在膀胱癌组织中高表达,CIP2A、OPN可以作为反映膀胱癌生物学行为的有效指标,提示了两者作为诊断标志物联合应用的潜在价值.
目的 探討蛋白燐痠酶2A的癌性抑製因子(CIP2A)、骨橋蛋白(OPN)在膀胱癌中的錶達及其在膀胱癌髮生髮展中的作用.方法 對2010年5月至2012年12月山東大學第二醫院泌尿外科收治的膀胱癌患者,採用反轉錄PCR (RT-PCR)檢測38例患者腫瘤組織和12例患者癌徬正常膀胱組織中CIP2A mRNA、OPN mRNA的錶達;免疫組化染色法檢測99例患者腫瘤組織和12例患者癌徬正常膀胱組織中CIP2A、OPN蛋白的錶達.結果 RT-PCR顯示38例膀胱癌患者中29例(76.32％)檢測到CIP2A mRNA的錶達,35例(92.11％)檢測到OPN mRNA的錶達,正常膀胱組織中二者均未有錶達(均P＜0.05).免疫組化結果顯示99例膀胱癌患者中有63例(63.64％) CIP2A蛋白暘性,84例(84.85％)OPN蛋白暘性;而正常膀胱組織中未檢測到CIP2A蛋白,2例OPN蛋白暘性(均P＜0.05).99例膀胱癌患者中,CIP2A蛋白與OPN蛋白錶達兩者均暘性的58例(58.59％),兩者均陰性的13例,CIP2A暘性而OPN陰性8例,CIP2A陰性而OPN暘性20例(r=0.300,P＜0.05).結論 CIP2A、OPN在膀胱癌組織中高錶達,CIP2A、OPN可以作為反映膀胱癌生物學行為的有效指標,提示瞭兩者作為診斷標誌物聯閤應用的潛在價值.
목적 탐토단백린산매2A적암성억제인자(CIP2A)、골교단백(OPN)재방광암중적표체급기재방광암발생발전중적작용.방법 대2010년5월지2012년12월산동대학제이의원비뇨외과수치적방광암환자,채용반전록PCR (RT-PCR)검측38례환자종류조직화12례환자암방정상방광조직중CIP2A mRNA、OPN mRNA적표체;면역조화염색법검측99례환자종류조직화12례환자암방정상방광조직중CIP2A、OPN단백적표체.결과 RT-PCR현시38례방광암환자중29례(76.32％)검측도CIP2A mRNA적표체,35례(92.11％)검측도OPN mRNA적표체,정상방광조직중이자균미유표체(균P＜0.05).면역조화결과현시99례방광암환자중유63례(63.64％) CIP2A단백양성,84례(84.85％)OPN단백양성;이정상방광조직중미검측도CIP2A단백,2례OPN단백양성(균P＜0.05).99례방광암환자중,CIP2A단백여OPN단백표체량자균양성적58례(58.59％),량자균음성적13례,CIP2A양성이OPN음성8례,CIP2A음성이OPN양성20례(r=0.300,P＜0.05).결론 CIP2A、OPN재방광암조직중고표체,CIP2A、OPN가이작위반영방광암생물학행위적유효지표,제시료량자작위진단표지물연합응용적잠재개치.
Objective To explore the expressions of cancerous inhibitor of protein phosphatase 2A (CIP2A) and osteopontin (OPN)and evaluate their roles in bladder cancer.Methods RNA was isolated from 38 cases of patients with bladder cancer and 12 cases of normal bladder tissue by TRIzol method from May 2010 to December 2012.And reverse transcription (RT)-PCR was used to detect the expressions of CIP2A and OPN.The expression levels of CIP2A and OPN in 99 cases of patients with bladder cancer and 12 cases of normal tissue were detected by immunohistochemical staining.Results The positive expression rate of CIP2A mRNA and OPN mRNA were 76.32％ (29/38) and 92.11％ (35/38)in bladder cancer while there was no expression in normal tissue (both P ＜ 0.05).The positive rates of CIP2A and OPN protein were 63.64％ (63/99) and 84.85％ (84/99) in cases of bladder cancer tissues while CIP2A was not detected in normal tissues.The positive expression rate of OPN in normal tissues was 2/12 (both P ＜ 0.05).The CIP2A and OPN proteins were both expressed in 58/99 cases of bladder cancer tissues while neither of them was expressed in 13 cases.In 8 cases,CIP2A was expressed while OPN was not.In another 20 cases,OPN was expressed while CIP2A was not(r =0.300,P ＜ 0.05).Conclusions The expression levels of CIP2A and OPN in tissue of bladder cancer are higher than those of normal controls.And CIP2A and OPN may be used as indicators of biological behaviors and serve as new molecular diagnostic markers for bladder cancer.
