CAJ | 학술논문

目的建立同位素稀释电感耦合等离子体质谱法(ID-ICP-MS)测定血清钾的分析方法.方法方法学建立研究.收集2010年朝阳医院检验科体检血清,制备成不同钾浓度的冰冻混合人血清.血清样品处理前加入待测元素39K的富集同位素41K,1％硝酸将血清准确稀释100倍,选择和优化ICP-MS分析条件后,测定其同位素比值,用内插法计算待测血清39K的浓度.3个不同浓度水平的血清各测定3批,评价该方法的精密度,美国国家标准与技术研究所(NIST)血清标准物质SRM909b-Ⅰ、Ⅱ评价正确度,两份血清加入不同浓度钾标物评价回收率,计算不确定度.结果血清钾测定的总变异系数CV为0.14％～0.11％；NIST血清标准物质SRM909b水平Ⅰ、Ⅱ的总CV为0.39％～0.32％,相对偏差分别为+0.12％、+0.49％.两份血清钾测定回收率为99.68％～100.12％.结论成功建立的血清钾同位素稀释电感耦合等离子体质谱法精密度高、准确性好,是准确定量测定无机元素的理想方法.
목적 건립동위소희석전감우합등리자체질보법(ID-ICP-MS)측정혈청갑적분석방법.방법 방법학건립연구.수집2010년조양의원검험과체검혈청,제비성불동갑농도적빙동혼합인혈청.혈청양품처리전가입대측원소39K적부집동위소41K,1％초산장혈청준학희석100배,선택화우화ICP-MS분석조건후,측정기동위소비치,용내삽법계산대측혈청39K적농도.3개불동농도수평적혈청각측정3비,평개해방법적정밀도,미국국가표준여기술연구소(NIST)혈청표준물질SRM909b-Ⅰ、Ⅱ평개정학도,량빈혈청가입불동농도갑표물평개회수솔,계산불학정도.결과 혈청갑측정적총변이계수CV위0.14％～0.11％；NIST혈청표준물질SRM909b수평Ⅰ、Ⅱ적총CV위0.39％～0.32％,상대편차분별위+0.12％、+0.49％.량빈혈청갑측정회수솔위99.68％～100.12％.결론 성공건립적혈청갑동위소희석전감우합등리자체질보법정밀도고、준학성호,시준학정량측정무궤원소적이상방법.
Objective To establish a method for the determination of serum potassium using isotope dilution inductively coupled plasma mass spectrometry.Methods Frozen human serum pools of different potassium concentrations were prepared from healthy donors of Chao-yang Hospital laboratory in 2010 Serum samples were diluted with 1％ HNO3 supplemented with 41K spike and isotope ratio was measured after selection and optimization of ICP-MS analysis conditions.Serum 39K concentrations were calculated by interpolation method.3 different serum samples were used to evaluate the precision of the method.Standard Reference Material 909b Ⅰ and Ⅱ of National Institute of Standards and Technology were used to evaluate the accuracy.The recovery was evaluated through 2 serum samples added standard material for potassium,uncertainty was calculated.Results The analytical precision of serum potassium measurement was 0.14％ to 0.11％.The results of SRM909b Ⅰ and Ⅱ gave the coefficients of variation (CVs) of 0.39％ to 0.32％.Data was consistent with the certified value and the CV was + 0.12％ and + 0.49％ respectively.The recovery was 99.68％ to 100.12％ for 2 different serum samples.Conclusions ID-ICP-MS method was successfully established to measure the serum potassium concerntration precisely and accurately,it is an ideal method for the accurate determination of inorganic elements.