CAJ | 학술논문

目的探讨多次强脉冲光(intense pulsed light,IPL)照射对皮肤成纤维细胞老化指标的影响,并用长波紫外线(ultraviolet A,UVA)作对比,观察多次强脉冲光照射能否造成细胞老化.方法实验分3组,一组朱接受照射作为对照组,其他两组分别接受UVA 9 J/cm2和IPL 15 J/cm2照射,每天1次,连续照射5d.在第6天,收集细胞,分别进行β半乳糖苷酶(SA-β-Gal)染色及细胞周期、细胞内活性氧和端粒长度的测定.结果连续5 d IPL照射后,SA-β-Gal染色和端粒长度与正常对照组相比,未见明显变化(P＞0.05).细胞周期中G1期所占百分比下降,细胞内活性氧下降(P＜0.05)；连续5 d UVA照射后,与正常对照组相比,SA-β-Gal染色阳性细胞的百分数增加,细胞周期中G1期所占百分比未见明显变化,细胞内活性氧上升,端粒长度缩短(P＜0.05).结论多次UVA照射可诱导细胞老化,但多次IPL照射不会出现细胞老化.
목적 탐토다차강맥충광(intense pulsed light,IPL)조사대피부성섬유세포노화지표적영향,병용장파자외선(ultraviolet A,UVA)작대비,관찰다차강맥충광조사능부조성세포노화.방법 실험분3조,일조주접수조사작위대조조,기타량조분별접수UVA 9 J/cm2화IPL 15 J/cm2조사,매천1차,련속조사5d.재제6천,수집세포,분별진행β반유당감매(SA-β-Gal)염색급세포주기、세포내활성양화단립장도적측정.결과 련속5 d IPL조사후,SA-β-Gal염색화단립장도여정상대조조상비,미견명현변화(P＞0.05).세포주기중G1기소점백분비하강,세포내활성양하강(P＜0.05)；련속5 d UVA조사후,여정상대조조상비,SA-β-Gal염색양성세포적백분수증가,세포주기중G1기소점백분비미견명현변화,세포내활성양상승,단립장도축단(P＜0.05).결론 다차UVA조사가유도세포노화,단다차IPL조사불회출현세포노화.
Objective To study the effect of multiple IPL treatment on cell senescence markers of skin fibroblasts using UVA as a control and to make clear whether the multiple IPL treatment may result in cell senescence.Methods Cells were divided into three groups: one group without irradiation as a control,one group receiving IPL treatment with 15 J per cm2,and the last group receiving UVA irradiation with 9 J per cm2.IPL and UVA irradiation were performed once a day during five days.On the sixth day,the cells were collected.Senescence-associated β-galactosidase (SA-β-Gal) staining,cell cycle,reactive oxygen species (ROS) and telomere length were determined.Results Our results showed that five consecutive days of IPL irradiation had no effect on the activity of SA-β-Gal and telomere length and decreased the G1 ％ of cell cycle and the level of ROS in comparison with the control group (P＜0.05).On the contrary,five consecutive days of UVA irradiation increased the activity of SA β Gal and the level of ROS,shortened the length of telomere and no obvious change in the G1 ％ of cell cycle in comparison with the control group.Conclusions Multiple UVA irradiations induce cell senescence.On the contrary,multiple IPL treatments could not induce cell senescence.