CAJ | 학술논문

目的探索电穿孔介导的基因治疗对兔下颌骨牵引成骨过程中骨形成蛋白-2(bone morphogenetic protein-2,BMP2)表达的影响.方法新西兰大白兔双侧下颌骨截骨,术后3d开始牵引,连续牵引7d后,将实验动物分为A、B、C、D、E5组,A、B、C组分别在牵引区注射2 μg(0.1 μg/μl)重组质粒pIRES-hVEGF165-hBMP2、pIRES-hBMP2、pIRES-hVEGF165.D组与E组分别注射相同剂量的空质粒(pIRES)和生理盐水(NS).各组分别于固定期第7、14、28天处死动物,取材行免疫组织化学检测BMP2的表达情况,并利用病理图像分析系统进行分析.结果 BMP2主要在肉芽组织中的炎细胞及新生幼稚骨小梁表面的细胞组织中表达.固定7d时表达最强烈,各时点基因治疗组明显强于对照组.结论电脉冲介导的基因治疗能使BMP2在牵引区的表达增强和表达时限延长并促进细胞的分裂增殖与分化,促进牵引区细胞基质的形成和新骨生成.
목적 탐색전천공개도적기인치료대토하합골견인성골과정중골형성단백-2(bone morphogenetic protein-2,BMP2)표체적영향.방법 신서란대백토쌍측하합골절골,술후3d개시견인,련속견인7d후,장실험동물분위A、B、C、D、E5조,A、B、C조분별재견인구주사2 μg(0.1 μg/μl)중조질립pIRES-hVEGF165-hBMP2、pIRES-hBMP2、pIRES-hVEGF165.D조여E조분별주사상동제량적공질립(pIRES)화생리염수(NS).각조분별우고정기제7、14、28천처사동물,취재행면역조직화학검측BMP2적표체정황,병이용병리도상분석계통진행분석.결과 BMP2주요재육아조직중적염세포급신생유치골소량표면적세포조직중표체.고정7d시표체최강렬,각시점기인치료조명현강우대조조.결론 전맥충개도적기인치료능사BMP2재견인구적표체증강화표체시한연장병촉진세포적분렬증식여분화,촉진견인구세포기질적형성화신골생성.
Objective To investigate the expression patterns of major bone growth factors and bone morphogenetic protein-2 (BMP2) in the distracted calluses following transfected gene during mandibular distraction osteogenesis in a rabbit model.Methods Bilateral mandibular osteotomies were performed in New-Zeland rabbits.After a latency of 3 days,the mandibles were elongated using distractors for 7 days.After the completion of distraction,the rabbits were randomly divided into 5 groups.Three animals each were sacrificed at the end of the delay phase,at 7,14,and 28 days after completion of distraction,respectively.The lengthened mandibles were harvested and processed for immunohistochemical detection of BMP2 expression,the mean optic densities and integral optical density of BMP2 positive cells were measured by computerized image analyzer.Results Elevated cellular expression of BMP2,in the distraction gap,was observed following mandibular distraction.BMP2 staining was mainly located in inflammatory cells,and the connective tissues arrounding the new bone.Their strongest expression was the 7th day,some of those growth factors expressed weakly or negatively.Conclusions Electroporation-mediated gene transfection can promote BMP2 expression effectively,which plays an important role in cell differentiation and proliferation during distraction osteo genesis.The BMP2 stimulates extracellular matrix synthesis,induces the proliferation and differentiation of fibroblasts and osteoblasts,which then promotes the new bone formation and repair.