CAJ | 학술논문

目的通过动物实验及组织学观察,研究评价所研制的异种肌腱基质填充材料的组织相容性.方法健康杂种犬15只,体质量10～13 kg,雌雄不限.于脊柱两侧皮下分别置入异种肌腱基质填充材料(实验组)和硅橡胶材料(对照组),术后14、30、60 d分别取材,进行大体观察和组织学观察.结果两组动物均在术后研究期间生存良好,置入部位均未见组织坏死、积液及化脓感染.术后14 d,实验组和对照组均见纤维组织增生包绕,炎性反应轻到中度；术后30 d,两组炎性反应轻微；术后60 d,实验组未见炎性反应,置入材料完整,未见肉芽肿.对照组置入硅胶材料周围有薄层纤维组织增生,炎性反应不明显.术后相同时间点,实验组与对照组组织学评分比较,差异无统计学意义(P＞0.05).结论异种肌腱基质填充材料具有良好的组织相容性.
목적 통과동물실험급조직학관찰,연구평개소연제적이충기건기질전충재료적조직상용성.방법 건강잡충견15지,체질량10～13 kg,자웅불한.우척주량측피하분별치입이충기건기질전충재료(실험조)화규상효재료(대조조),술후14、30、60 d분별취재,진행대체관찰화조직학관찰.결과 량조동물균재술후연구기간생존량호,치입부위균미견조직배사、적액급화농감염.술후14 d,실험조화대조조균견섬유조직증생포요,염성반응경도중도；술후30 d,량조염성반응경미；술후60 d,실험조미견염성반응,치입재료완정,미견육아종.대조조치입규효재료주위유박층섬유조직증생,염성반응불명현.술후상동시간점,실험조여대조조조직학평분비교,차이무통계학의의(P＞0.05).결론 이충기건기질전충재료구유량호적조직상용성.
Objective To evaluate the histocompatibility of novel manufactured xenogenic tendon matrix materials by an animal experimental study.Methods The study was conducted on 15 dogs,weighing 10-13 kg.The prepared xenogenic tendon matrix materials were implanted into the bilateral area of spine in dogs subcutaneously (experimental group),and the implantation of silicon served as control group.The animals were killed 14,30,60 days after surgery and the specimens were processed in laboratory to receive gross and histology observation.The histological sections were stained with hematoxylin-eosin and analyzed by light microscopy.Scores were assigned to the inflammatory process and statistically compared by two related samples with non-parametric test.Results All dogs survived well during the embedded test.There was no tissue necrosis,effusion or inflammation at all implantation sites in both groups during the test.The xenogenic implant materials promoted slight to moderate inflammation process after 14 days,with no statistically significant difference compared to the control.However,after 30 days,there was a regression of inflammation.After 60 days,it was observed the presence of well-organized connective tissue,and few inflammatory cells.Score evaluation of inflammation response at different time after operation of two groups showed no statistically significant difference (P＞0.05).Conclusions The new xenogenic tendon matrix materials are considered biocompatible with subcutaneous tissue.