CAJ | 학술논문

目的探讨肉毒毒素与透明质酸联合应用对皮肤真皮层成纤维细胞增长和胶原沉积的影响.方法实验采用新西兰兔建立动物模型,设立透明质酸(HA实验组)、A型肉毒毒素(BTX-A实验组)及二者联合(联合实验组),注射3个实验组和对照组(生理盐水),于第1、2、4周,通过病理切片HE染色,观察各实验组对真皮层成纤维细胞影响的差异,用Ⅰ、Ⅲ型胶原免疫组织化学染色方法,评估各实验组对真皮层胶原含量影响的差异.结果联合实验组第2、4周成纤维细胞数目显著高于对照组(P＜0.05),免疫组织化学切片Ⅰ、Ⅲ型胶原平均积分吸光度(A值)与对照组差异无统计学意义(P＞0.05).结论 BTX-A和HA联合局部注射使真皮层成纤维细胞数目增加,且有效缓解BTX-A单独注射引起的对Ⅰ、Ⅲ型胶原沉积的抑制作用,从而提高肉毒毒素和透明质酸单独应用的治疗效果.
목적 탐토육독독소여투명질산연합응용대피부진피층성섬유세포증장화효원침적적영향.방법 실험채용신서란토건립동물모형,설립투명질산(HA실험조)、A형육독독소(BTX-A실험조)급이자연합(연합실험조),주사3개실험조화대조조(생리염수),우제1、2、4주,통과병리절편HE염색,관찰각실험조대진피층성섬유세포영향적차이,용Ⅰ、Ⅲ형효원면역조직화학염색방법,평고각실험조대진피층효원함량영향적차이.결과 연합실험조제2、4주성섬유세포수목현저고우대조조(P＜0.05),면역조직화학절편Ⅰ、Ⅲ형효원평균적분흡광도(A치)여대조조차이무통계학의의(P＞0.05).결론 BTX-A화HA연합국부주사사진피층성섬유세포수목증가,차유효완해BTX-A단독주사인기적대Ⅰ、Ⅲ형효원침적적억제작용,종이제고육독독소화투명질산단독응용적치료효과.
Objective To study the effect on fibroblastic proliferation and collagen deposition in dermal layer with combined treatment of botulinum toxin type A (BTX-A) and hyaluronic acid (HA).Methods Six male New Zealand rabbits were selected to establish animal models and subcutaneous separated injections were carried out with saline (control group),HA (HA group),BTX-A (BTX-A group) and combination treatment of BTX-A and HA (combination group).The observation of differences between these four treatment groups was conducted on day 7,day 14,and day 28 respectively after the experimental treatment,including the number and morphology of fibroblasts with HE staining and the deposition of type Ⅰ and type Ⅲ collagen with immunohistochemical staining under light microscope.Results The group with combined treatment of BTX-A and HA had significantly larger number of fibroblasts at all the three observation time points (P＜0.05).In terms of average IOD from immunohistochemical stained section of production of type Ⅰ and type Ⅲ collagen,the group with combined treatment exerted no significant differences as compared to the control group (P＞0.05).Conclusions The combined treatment of BTX-A and HA will promote the proliferation of fibroblasts and to some degree offset its inhibitory effect on production of collagen.The combination will improve the efficacy of any separated treatment with BTX-A or HA.