中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2013年
1期
43-46
,共4页
亓玉青%李天航%范丽云%刘全忠
亓玉青%李天航%範麗雲%劉全忠
기옥청%리천항%범려운%류전충
基质金属蛋白酶-1%透明质酸合成酶-2%成纤维细胞%皮肤老化
基質金屬蛋白酶-1%透明質痠閤成酶-2%成纖維細胞%皮膚老化
기질금속단백매-1%투명질산합성매-2%성섬유세포%피부노화
Matrix metalloproteinase-1 (MMP-1)%Hyaluronic acid synthetase-2 (HAS-2)%Fibroblast%Skin aging
目的 通过研究雌二醇、维生素E、维生素C对体外培养的人皮肤成纤维细胞基质金属蛋白酶-1(MMP-1) mRNA、透明质酸合成酶-2(HAS-2) mRNA转录水平的影响,探讨不同药物改善皮肤老化的可能机制.方法 将不同浓度的雌二醇、维生素E、维生素C分别加入体外培养的人皮肤成纤维细胞,逆转录-聚合酶链反应(RT-PCR)检测不同物质对成纤维细胞中HAS-2 mRNA、MMP-1 mRNA表达水平的影响.结果 雌二醇、维生素C对MMP 1 mRNA表达的影响:高剂量组、低剂量组与对照组的差异有统计学意义(P＜0.05),低剂量组与高剂量组差异无统计学意义(P＞0.05).维生素E、维生素C对HAS-2 mRNA表达的影响:高剂量组、低剂量组与对照组的差异有统计学意义(P＜0.05),低剂量组与高剂量组差异无统计学意义(P＞0.05).结论 雌二醇、维生素C可以下调MMP-1基因的转录水平,减少后者对皮肤胶原的降解；维生素E、维生素C可以上调HAS-2基因的转录水平,可能增加透明质酸的合成,从而改善皮肤老化.
目的 通過研究雌二醇、維生素E、維生素C對體外培養的人皮膚成纖維細胞基質金屬蛋白酶-1(MMP-1) mRNA、透明質痠閤成酶-2(HAS-2) mRNA轉錄水平的影響,探討不同藥物改善皮膚老化的可能機製.方法 將不同濃度的雌二醇、維生素E、維生素C分彆加入體外培養的人皮膚成纖維細胞,逆轉錄-聚閤酶鏈反應(RT-PCR)檢測不同物質對成纖維細胞中HAS-2 mRNA、MMP-1 mRNA錶達水平的影響.結果 雌二醇、維生素C對MMP 1 mRNA錶達的影響:高劑量組、低劑量組與對照組的差異有統計學意義(P＜0.05),低劑量組與高劑量組差異無統計學意義(P＞0.05).維生素E、維生素C對HAS-2 mRNA錶達的影響:高劑量組、低劑量組與對照組的差異有統計學意義(P＜0.05),低劑量組與高劑量組差異無統計學意義(P＞0.05).結論 雌二醇、維生素C可以下調MMP-1基因的轉錄水平,減少後者對皮膚膠原的降解；維生素E、維生素C可以上調HAS-2基因的轉錄水平,可能增加透明質痠的閤成,從而改善皮膚老化.
목적 통과연구자이순、유생소E、유생소C대체외배양적인피부성섬유세포기질금속단백매-1(MMP-1) mRNA、투명질산합성매-2(HAS-2) mRNA전록수평적영향,탐토불동약물개선피부노화적가능궤제.방법 장불동농도적자이순、유생소E、유생소C분별가입체외배양적인피부성섬유세포,역전록-취합매련반응(RT-PCR)검측불동물질대성섬유세포중HAS-2 mRNA、MMP-1 mRNA표체수평적영향.결과 자이순、유생소C대MMP 1 mRNA표체적영향:고제량조、저제량조여대조조적차이유통계학의의(P＜0.05),저제량조여고제량조차이무통계학의의(P＞0.05).유생소E、유생소C대HAS-2 mRNA표체적영향:고제량조、저제량조여대조조적차이유통계학의의(P＜0.05),저제량조여고제량조차이무통계학의의(P＞0.05).결론 자이순、유생소C가이하조MMP-1기인적전록수평,감소후자대피부효원적강해；유생소E、유생소C가이상조HAS-2기인적전록수평,가능증가투명질산적합성,종이개선피부노화.
Objective To investigate the possible mechanisms using different chemicals to improve the skin aging by the effect of estrogen,vitamin E,vitamin C on the transcriptional level of MMP-1 and HAS-2 mRNAs in cultured human skin fibroblast in vitro.Methods The different concentrations of estrogen,vitamin E,vitamin C were put into cultured human skin fibroblasts.After 24 hours,RT-PCR was used to detect the effects of the different chemicals on the transcriptional levels of the HAS-2 and MMP-1 mRNAs in fibroblasts.Results The effects of estrogen and vitamin C on the MMP-1 mRNA had significant difference between the high dose group and blank control group,and between the low dose group and blank control group (P＜0.05) ; the difference between the low dose group and high dose group was not statistically significant (P＞0.05).The effects of vitamin E and vitamin C on Has-2 mRNA also had significant difference between the high dose group and the blank control group,and between the low dose group and blank control group (P＜0.05),but the difference between the low dose group and high dose group was not statistically significant (P＞0.05).Conclusions Estrogen and vitamin C can reduce the transcription of MMP-1 gene,which decreases the degradation of collagen in the skin; vitamin E and vitamin C can increase the transcription of HAS-2 gene,which may possibly increase the synthesis of hyaluronic acid to alleviate the skin aging.
