CAJ | 학술논문

目的观察脂质体携载前列腺素E1(PGE1)对扩张皮瓣存活的干预机制.方法采用中国小型猪为实验动物,实验分2组:脂质体携载PGE1组(实验组)及未用药对照组.在其背部形成8 cm×2 cm扩张皮瓣,通过图像分析技术及Real-time PCR技术,观察脂质体携载PGE1对皮瓣存活的影响及作用机制.结果实验组与对照组相比,皮瓣存活率增高(P＜0.01),血管内皮细胞生长因子(VEGF)、iNOS、Ang-1基因表达均降低,β1转化生长因子(TGF-β1)基因表达增高,但组间差异无统计学意义(P＞0.05).结论应用脂质体携载PGE1可以提高扩张皮瓣存活率,但脂质体携载PGE1减轻扩张皮瓣缺血-再灌注损伤的机制不是通过调节VEGF、iNOS、Ang-1及TGF-β1基因的表达实现的.
목적 관찰지질체휴재전렬선소E1(PGE1)대확장피판존활적간예궤제.방법 채용중국소형저위실험동물,실험분2조:지질체휴재PGE1조(실험조)급미용약대조조.재기배부형성8 cm×2 cm확장피판,통과도상분석기술급Real-time PCR기술,관찰지질체휴재PGE1대피판존활적영향급작용궤제.결과 실험조여대조조상비,피판존활솔증고(P＜0.01),혈관내피세포생장인자(VEGF)、iNOS、Ang-1기인표체균강저,β1전화생장인자(TGF-β1)기인표체증고,단조간차이무통계학의의(P＞0.05).결론 응용지질체휴재PGE1가이제고확장피판존활솔,단지질체휴재PGE1감경확장피판결혈-재관주손상적궤제불시통과조절VEGF、iNOS、Ang-1급TGF-β1기인적표체실현적.
Objective To investigate the mechanism of liposome PGE1 on the survival of the expanded flap.Methods The minipigs were set up as an animal model.They were divided into 2 groups.The expanded flaps (8 cm ×2 cm) were designed and elevated on the back of an minipig.An image analysis technology was used to evaluate the survival rate of the expanded flap,while realtimePCR technique was used to investigate the molecular mechanism.Results The survival area of the expanded flap in the Lipo PGE1 group was significantly enlarged (P＜0.01) compared with the control group.The VEGF,iNOS and Ang-1 gene expression decreased,but TGF-β1 gene expression increased.But there was no significant difference between two groups (P＞0.05).Conclusions The Lipo PGE1 could promote survival rate of the expanded flap,but the mechanism does not involve the regulation of VEGF,iNOS,Ang-1 and TGF-β1 gene expression.