中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2013年
6期
453-456
,共4页
周滨%吴国平%胡纯兵%何小川
週濱%吳國平%鬍純兵%何小川
주빈%오국평%호순병%하소천
乳头内陷%弹性外牵引%雌性小型猪%碱性成纤维细胞生长因子%β1转化生长因子
乳頭內陷%彈性外牽引%雌性小型豬%堿性成纖維細胞生長因子%β1轉化生長因子
유두내함%탄성외견인%자성소형저%감성성섬유세포생장인자%β1전화생장인자
Elastic distraction%Inverted nipple%Female minipig%Basic fibroblast growth factor (bFGF)%Transforming growth factor-beta 1 (TGF-β1)
目的 观察持续弹性外牵引过程中雌性小型猪乳头及其支撑组织中碱性成纤维细胞生长因子(bFGF)和β1转化生长因子(TGF-β1)表达的变化,探讨持续弹性外牵引矫治乳头内陷的机制.方法 选用3只雌性小型猪,每只各12个乳头;随机选取4个乳头作为空白对照组,其余乳头安置自制弹性可调式牵拉矫治器进行持续外牵引.于牵引后第2、4、8、12周分别各切取1个空白对照组乳头和2个被牵引乳头,行免疫组织化学染色,观察不同时期bFGF和TGF-β1的表达情况.结果 牵引后各时期bFGF及TGF-β1的表达均较对照组明显增加,差异有统计学意义(P＜0.01).bFGF的阳性表达在牵引4周时达到最高水平,与12周时相比差异有统计学意义(P＜0.05).TGF-β1的阳性表达在牵引2周时达到最高水平,同8周、12周牵引组相比差异有统计学意义(P＜0.05).bFGF和TGF-β1表达呈显著正相关.结论 持续弹性外牵引可刺激小型猪乳头组织中bFGF和TGF-β1的合成和分泌增加.
目的 觀察持續彈性外牽引過程中雌性小型豬乳頭及其支撐組織中堿性成纖維細胞生長因子(bFGF)和β1轉化生長因子(TGF-β1)錶達的變化,探討持續彈性外牽引矯治乳頭內陷的機製.方法 選用3隻雌性小型豬,每隻各12箇乳頭;隨機選取4箇乳頭作為空白對照組,其餘乳頭安置自製彈性可調式牽拉矯治器進行持續外牽引.于牽引後第2、4、8、12週分彆各切取1箇空白對照組乳頭和2箇被牽引乳頭,行免疫組織化學染色,觀察不同時期bFGF和TGF-β1的錶達情況.結果 牽引後各時期bFGF及TGF-β1的錶達均較對照組明顯增加,差異有統計學意義(P＜0.01).bFGF的暘性錶達在牽引4週時達到最高水平,與12週時相比差異有統計學意義(P＜0.05).TGF-β1的暘性錶達在牽引2週時達到最高水平,同8週、12週牽引組相比差異有統計學意義(P＜0.05).bFGF和TGF-β1錶達呈顯著正相關.結論 持續彈性外牽引可刺激小型豬乳頭組織中bFGF和TGF-β1的閤成和分泌增加.
목적 관찰지속탄성외견인과정중자성소형저유두급기지탱조직중감성성섬유세포생장인자(bFGF)화β1전화생장인자(TGF-β1)표체적변화,탐토지속탄성외견인교치유두내함적궤제.방법 선용3지자성소형저,매지각12개유두;수궤선취4개유두작위공백대조조,기여유두안치자제탄성가조식견랍교치기진행지속외견인.우견인후제2、4、8、12주분별각절취1개공백대조조유두화2개피견인유두,행면역조직화학염색,관찰불동시기bFGF화TGF-β1적표체정황.결과 견인후각시기bFGF급TGF-β1적표체균교대조조명현증가,차이유통계학의의(P＜0.01).bFGF적양성표체재견인4주시체도최고수평,여12주시상비차이유통계학의의(P＜0.05).TGF-β1적양성표체재견인2주시체도최고수평,동8주、12주견인조상비차이유통계학의의(P＜0.05).bFGF화TGF-β1표체정현저정상관.결론 지속탄성외견인가자격소형저유두조직중bFGF화TGF-β1적합성화분비증가.
Objective To observe the effect of continuous elastic outside distraction on the expression of basic fibroblast growth factor (bFGF) and transforming growth factor-beta 1 (TGF-β1) in the female porcine nipples,and to further investigate the mechanism of continuous elastic outside distraction stimulation on the proliferation of supporting tissue in nipples.Methods Three female minipigs were employed,each of them had 12 nipples.Four nipples of each minipig were used as control group,while the other eight nipples were continuously distracted with prefabricated instruments.One nipple without distraction and two distracted nipples of each minipig were excised at 2,4,8,and 12 weeks after operation under general anesthesia respectively.The excised nipples were prepared for immunohistochemical staining to observe the expression of bFGF and TGF-β1.Image analysis software was employed for quantitative analysis.Results bFGF and TGF-β1 expression of control groups was weaker than those of the distracted groups at any time (P＜0.01).The expression of bFGF increased gradually after tracted and reached the peak at 4 weeks.There was significant difference (P＜0.05) in the expression of bFGF between 4 weeks and 12 weeks groups.The expression of TGF-β1 was increased to the peak after 2 weeks.The expression of TGF-β1 between 8 weeks and 12 weeks groups and 2 weeks group had statistical significance (P＜0.05).The expression of bFGF and TGF-β1 had a positive correlation during the traction.Conclusions Continuous elastic outside distraction can promote female minipig nipple to synthesis and secretion of b-FGF and TGF-β1.
