中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2013年
6期
697-700
,共4页
甄建新%邹红岩%金士正%高素青%王大明%何柳媚%邓志辉
甄建新%鄒紅巖%金士正%高素青%王大明%何柳媚%鄧誌輝
견건신%추홍암%금사정%고소청%왕대명%하류미%산지휘
人类白细胞抗原%无关供-受者对%HLA-DPA1%HLA-DPB1%测序分型%匹配程度
人類白細胞抗原%無關供-受者對%HLA-DPA1%HLA-DPB1%測序分型%匹配程度
인류백세포항원%무관공-수자대%HLA-DPA1%HLA-DPB1%측서분형%필배정도
Human leukocyte antigens%Unrelated donor-recipient pair%HLA-DPA1 gene%HLA-DPB1 gene%Sequence-based typing%Matching status
目的 研究HLA-A、-B、-C、-DRB1和-DQB1等位基因全相合的无关供-受者对HLA-DPA1和-DPB1基因的匹配性.方法 对76对HLA-A、-B、-C、-DRB1和-DQB1等位基因10/10相合的无关供-受者样本进行HLA-DPA1和-DPB1基因测序分型,从等位基因水平统计和分析HLA-DPA1和-DPB1基因匹配的比例和特点.结果 单个HLA-DPA1、-DPB1基因高分辨水平完全相合的比例分别为17.1％、9.2％,完全相合的比例均较低.HLA-DPA1等位基因半相合的比例占绝大多数(73.7％),完全不合的比例为9.2％;具有高度多态性的HLA-DPB1基因半相合的比例为57.9％,而完全不合的比例为32.9％.统计HLA-DPA1+-DPB1等位基因的匹配率,2/4相合的比例最高(51.4％),4/4相合的比例仅为5.6％,而0/4相合的比例为8.3％.结论 HLA-A、-B、-C、-DRB1和-DQB1等位基因相合的无关供-受者对HLA-DPA1和-DPB1基因匹配率尚较低,HLA-DPA1和-DPB1基因匹配程度对无关供者造血干细胞移植的影响应引起临床的重视和探讨.
目的 研究HLA-A、-B、-C、-DRB1和-DQB1等位基因全相閤的無關供-受者對HLA-DPA1和-DPB1基因的匹配性.方法 對76對HLA-A、-B、-C、-DRB1和-DQB1等位基因10/10相閤的無關供-受者樣本進行HLA-DPA1和-DPB1基因測序分型,從等位基因水平統計和分析HLA-DPA1和-DPB1基因匹配的比例和特點.結果 單箇HLA-DPA1、-DPB1基因高分辨水平完全相閤的比例分彆為17.1％、9.2％,完全相閤的比例均較低.HLA-DPA1等位基因半相閤的比例佔絕大多數(73.7％),完全不閤的比例為9.2％;具有高度多態性的HLA-DPB1基因半相閤的比例為57.9％,而完全不閤的比例為32.9％.統計HLA-DPA1+-DPB1等位基因的匹配率,2/4相閤的比例最高(51.4％),4/4相閤的比例僅為5.6％,而0/4相閤的比例為8.3％.結論 HLA-A、-B、-C、-DRB1和-DQB1等位基因相閤的無關供-受者對HLA-DPA1和-DPB1基因匹配率尚較低,HLA-DPA1和-DPB1基因匹配程度對無關供者造血榦細胞移植的影響應引起臨床的重視和探討.
목적 연구HLA-A、-B、-C、-DRB1화-DQB1등위기인전상합적무관공-수자대HLA-DPA1화-DPB1기인적필배성.방법 대76대HLA-A、-B、-C、-DRB1화-DQB1등위기인10/10상합적무관공-수자양본진행HLA-DPA1화-DPB1기인측서분형,종등위기인수평통계화분석HLA-DPA1화-DPB1기인필배적비례화특점.결과 단개HLA-DPA1、-DPB1기인고분변수평완전상합적비례분별위17.1％、9.2％,완전상합적비례균교저.HLA-DPA1등위기인반상합적비례점절대다수(73.7％),완전불합적비례위9.2％;구유고도다태성적HLA-DPB1기인반상합적비례위57.9％,이완전불합적비례위32.9％.통계HLA-DPA1+-DPB1등위기인적필배솔,2/4상합적비례최고(51.4％),4/4상합적비례부위5.6％,이0/4상합적비례위8.3％.결론 HLA-A、-B、-C、-DRB1화-DQB1등위기인상합적무관공-수자대HLA-DPA1화-DPB1기인필배솔상교저,HLA-DPA1화-DPB1기인필배정도대무관공자조혈간세포이식적영향응인기림상적중시화탐토.
Objective To analyze the status of HLA-DPA1 and DPB1 matching for unrelated donor-recipient pairs matched at high-resolution allele level for HLA-A,B,C,DRB1 and DQB1 loci.Methods A total of 76 unrelated donor-recipient pairs matching at allele level for HLA-A,B,C,DRBland DQB1 loci were subjected to HLA-DPA1 and DPB1 sequence-based typing (SBT).HLA-DPAland DPB1 matching status at high-resolution allelic level was also analyzed.Results The allelic identity ratio for single HLA-DPA1 and DPB1 were 17.1％ and 9.2％,respectively.HLA-DPA1 and DPB1 allelic identity ratio were both very low.The majority of unrelated donor-recipient pairs (73.7 ％) had an incompatibility at 1 HLA-DPA1 allele,9.2％ of pairs had an incompatibility at 2 DPA1 alleles.As for the high-polymorphic HLA-DPB1 gene,57.9 ％ of studied donor-recipient pairs had an incompatibility at 1 HLA-DPB1 allele,almost 1/3 (32.9％) of them were completely incompatible.When HLA-DPA1 and DPB1 genes were analyzed together,the donor-recipient pairs matched at 2/4 was the most common (51.4％),4/4 allelic complete matched pairs accounted for 5.6％,and 0/4 matched pairs accounted for 8.3％.Conclusion Our results indicated that the ratio of HLA-DPA1 and DPB1 complete match in the unrelated donor-recipient pairs matching at allelic level for HLA-A,B,C,DRB1and DQB1 loci were very low.The effect of HLA-DPA1 and DPB1 matching status on clinical unrelated stem cell transplantation still needs to be elucidated.
