中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2013年
6期
701-705
,共5页
陈孝倩%吴琦%谢双双%赵卫军%吴存造%蔡勇%夏鹏%杨亦荣%陈必成
陳孝倩%吳琦%謝雙雙%趙衛軍%吳存造%蔡勇%夏鵬%楊亦榮%陳必成
진효천%오기%사쌍쌍%조위군%오존조%채용%하붕%양역영%진필성
杀伤细胞免疫球蛋白样受体%肾移植%基因型%多态性
殺傷細胞免疫毬蛋白樣受體%腎移植%基因型%多態性
살상세포면역구단백양수체%신이식%기인형%다태성
Killer cell immunoglobulin-like receptor%Renal transplantation%Genotype%Polymorphism
目的 分析浙江南部地区同种异体肾移植受者杀伤细胞免疫球蛋白样受体(killer cell immunoglobulin-like receptor,KIR)基因多态性的分布情况.方法 采用序列特异性引物聚合酶链反应检测浙江南部地区无血缘关系的416例汉族肾移植受者的KIR基因型,并与其他地区人群的基因型相比较.结果 共检出16个已知的KIR基因,其中2DL4、3DL2～3、3DP1在所有个体中均表达;沉默基因2DP1的基因型频率为99％.其它高频的基因依次为2DL1、2DL3、3DL1、2DS4 (92.1％～98.8％).抑制型基因2DL2基因表型频率(34.6％)高于江浙沪汉族人群(P＜0.05);比较其他基因表型频率差异无统计学意义(P＞0.05).检出的41种KIR基因型中有3种基因型未见文献报道.KIR基因型以AA1型频率最高(43.51％),明显低于已报道的浙江北部和江苏地区对照人群.结论 浙南地区肾移植受者KIR基因分布与华东地区健康对照人群基本一致,但也表现出其独特的KIR基因多态性特征.
目的 分析浙江南部地區同種異體腎移植受者殺傷細胞免疫毬蛋白樣受體(killer cell immunoglobulin-like receptor,KIR)基因多態性的分佈情況.方法 採用序列特異性引物聚閤酶鏈反應檢測浙江南部地區無血緣關繫的416例漢族腎移植受者的KIR基因型,併與其他地區人群的基因型相比較.結果 共檢齣16箇已知的KIR基因,其中2DL4、3DL2～3、3DP1在所有箇體中均錶達;沉默基因2DP1的基因型頻率為99％.其它高頻的基因依次為2DL1、2DL3、3DL1、2DS4 (92.1％～98.8％).抑製型基因2DL2基因錶型頻率(34.6％)高于江浙滬漢族人群(P＜0.05);比較其他基因錶型頻率差異無統計學意義(P＞0.05).檢齣的41種KIR基因型中有3種基因型未見文獻報道.KIR基因型以AA1型頻率最高(43.51％),明顯低于已報道的浙江北部和江囌地區對照人群.結論 浙南地區腎移植受者KIR基因分佈與華東地區健康對照人群基本一緻,但也錶現齣其獨特的KIR基因多態性特徵.
목적 분석절강남부지구동충이체신이식수자살상세포면역구단백양수체(killer cell immunoglobulin-like receptor,KIR)기인다태성적분포정황.방법 채용서렬특이성인물취합매련반응검측절강남부지구무혈연관계적416례한족신이식수자적KIR기인형,병여기타지구인군적기인형상비교.결과 공검출16개이지적KIR기인,기중2DL4、3DL2～3、3DP1재소유개체중균표체;침묵기인2DP1적기인형빈솔위99％.기타고빈적기인의차위2DL1、2DL3、3DL1、2DS4 (92.1％～98.8％).억제형기인2DL2기인표형빈솔(34.6％)고우강절호한족인군(P＜0.05);비교기타기인표형빈솔차이무통계학의의(P＞0.05).검출적41충KIR기인형중유3충기인형미견문헌보도.KIR기인형이AA1형빈솔최고(43.51％),명현저우이보도적절강북부화강소지구대조인군.결론 절남지구신이식수자KIR기인분포여화동지구건강대조인군기본일치,단야표현출기독특적KIR기인다태성특정.
Objective To investigate polymorphisms of killer cell immunoglobulin-like receptor gene (KIR) in renal transplant recipients from southern Zhejiang.Methods KIR genotypes were analyzed by PCR-SSP in 416 renal transplant recipients,and the genotype frequencies were compared with populations from Eastern China and worldwide.Results All 16 known KIR genes were detected in the renal transplant recipients,and KIR2DL4,3DL2～3,3PD1 were found in all.As a pseudogene,2DP1 has a high genotype frequency (99 ％).The frequencies of KIR2DL1,2DL3,3DL1,2DS4 have ranged from 92.1％ to 98.8 ％.Compared with 11 groups in Eastern China and other countries,the KIR2DL2 phenotype frequency was higher (34.6％) than those of Shanghai,Zhejiang and Jiangsu populations (P＜ 0.05).Among 41 genotypes,three have not been reported previously.The most common genotype was AA1,with a frequency of 43.51％,which was significantly lower than those of Jiangsu and Northern Zhejiang.Conclusion Renal transplant recipients from southern Zhejiang share similar features with Eastern China Han population with regard to KIR polymorphisms,but also have unique frequencies for KIR genotypes.
