中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2013年
4期
262-267
,共6页
王娜娜%王建业%史晓红%张耀光%刘铭%王鑫%惠娟%陈鑫%梁思颖
王娜娜%王建業%史曉紅%張耀光%劉銘%王鑫%惠娟%陳鑫%樑思穎
왕나나%왕건업%사효홍%장요광%류명%왕흠%혜연%진흠%량사영
前列腺肿瘤%基因,TET2%基因,LMTK2%基因,FAM84B%多态性,单核苷酸
前列腺腫瘤%基因,TET2%基因,LMTK2%基因,FAM84B%多態性,單覈苷痠
전렬선종류%기인,TET2%기인,LMTK2%기인,FAM84B%다태성,단핵감산
Prostatic neoplasms%Gene,TET2%Gene,LMTK2%Gene,FAM84B%Polymorphism,single nucleotide
目的 探索TET2基因(rs7679673,A)、LMTK2基因(rs6465657,T)和FAM84B基因(rs12543663,C)的常见变异类型与前列腺癌患病风险的关系,分析前列腺癌患者的基因型与临床特征和生活习惯的关系.方法 采用病例对照研究设计,比较病例组和对照组TET2基因(rs7679673,A)、LMTK2基因(rs6465657,T)和FAM84B基因(rs12543663,C)的等位基因和基因型频率的差异,采用x2检验分析各基因型与患者的确诊年龄、体质指数、Gleason评分、血清前列腺特异性抗原浓度、肿瘤分期等的关系,采用多因子降维(MDR)方法分析基因-基因交互作用.结果 FAM84B基因(rs12543663,C)的CC+ CA基因型在病例组和对照组中的频率分布差异有统计学意义(P=0.046).TET2基因(rs7679673,A)和LMTK2基因(rs6465657,T)的AA、AC和CC基因型和等位基因在病例组和对照组中的频率分布差异均无统计学意义(均P＞0.05).FAM84B基因(rs12543663,C)的CC+AC基因型在不同肿瘤分期前列腺癌患者中的分布频率,差异有统计学意义(P =0.002).TET2基因的AA、AC和CC基因型在不同吸烟和饮酒情况的前列腺癌患者中的分布频率,差异均有统计学意义(均P＜0.05).LMTK2(rs6465657,T)基因的TT+TC和CC基因型在是否接受手术治疗的前列腺癌患者中的分布频率,差异有统计学意义(P =0.003).树状图分析结果显示,TET2(rs7679673,A)和LMTK2(rs6465657,T)基因位点之间距离更近,存在更强的基因-基因交互作用.MDR方法验证最佳模型的检验平衡精度为0.5120,交叉验证一致性为100％(P=0.0270).结论 FAM84B基因可能与中国人前列腺癌患病风险和肿瘤分期有关,TET2、LMTK2和FAM84B基因的协同作用可能与前列腺癌的患病风险有关.
目的 探索TET2基因(rs7679673,A)、LMTK2基因(rs6465657,T)和FAM84B基因(rs12543663,C)的常見變異類型與前列腺癌患病風險的關繫,分析前列腺癌患者的基因型與臨床特徵和生活習慣的關繫.方法 採用病例對照研究設計,比較病例組和對照組TET2基因(rs7679673,A)、LMTK2基因(rs6465657,T)和FAM84B基因(rs12543663,C)的等位基因和基因型頻率的差異,採用x2檢驗分析各基因型與患者的確診年齡、體質指數、Gleason評分、血清前列腺特異性抗原濃度、腫瘤分期等的關繫,採用多因子降維(MDR)方法分析基因-基因交互作用.結果 FAM84B基因(rs12543663,C)的CC+ CA基因型在病例組和對照組中的頻率分佈差異有統計學意義(P=0.046).TET2基因(rs7679673,A)和LMTK2基因(rs6465657,T)的AA、AC和CC基因型和等位基因在病例組和對照組中的頻率分佈差異均無統計學意義(均P＞0.05).FAM84B基因(rs12543663,C)的CC+AC基因型在不同腫瘤分期前列腺癌患者中的分佈頻率,差異有統計學意義(P =0.002).TET2基因的AA、AC和CC基因型在不同吸煙和飲酒情況的前列腺癌患者中的分佈頻率,差異均有統計學意義(均P＜0.05).LMTK2(rs6465657,T)基因的TT+TC和CC基因型在是否接受手術治療的前列腺癌患者中的分佈頻率,差異有統計學意義(P =0.003).樹狀圖分析結果顯示,TET2(rs7679673,A)和LMTK2(rs6465657,T)基因位點之間距離更近,存在更彊的基因-基因交互作用.MDR方法驗證最佳模型的檢驗平衡精度為0.5120,交扠驗證一緻性為100％(P=0.0270).結論 FAM84B基因可能與中國人前列腺癌患病風險和腫瘤分期有關,TET2、LMTK2和FAM84B基因的協同作用可能與前列腺癌的患病風險有關.
목적 탐색TET2기인(rs7679673,A)、LMTK2기인(rs6465657,T)화FAM84B기인(rs12543663,C)적상견변이류형여전렬선암환병풍험적관계,분석전렬선암환자적기인형여림상특정화생활습관적관계.방법 채용병례대조연구설계,비교병례조화대조조TET2기인(rs7679673,A)、LMTK2기인(rs6465657,T)화FAM84B기인(rs12543663,C)적등위기인화기인형빈솔적차이,채용x2검험분석각기인형여환자적학진년령、체질지수、Gleason평분、혈청전렬선특이성항원농도、종류분기등적관계,채용다인자강유(MDR)방법분석기인-기인교호작용.결과 FAM84B기인(rs12543663,C)적CC+ CA기인형재병례조화대조조중적빈솔분포차이유통계학의의(P=0.046).TET2기인(rs7679673,A)화LMTK2기인(rs6465657,T)적AA、AC화CC기인형화등위기인재병례조화대조조중적빈솔분포차이균무통계학의의(균P＞0.05).FAM84B기인(rs12543663,C)적CC+AC기인형재불동종류분기전렬선암환자중적분포빈솔,차이유통계학의의(P =0.002).TET2기인적AA、AC화CC기인형재불동흡연화음주정황적전렬선암환자중적분포빈솔,차이균유통계학의의(균P＜0.05).LMTK2(rs6465657,T)기인적TT+TC화CC기인형재시부접수수술치료적전렬선암환자중적분포빈솔,차이유통계학의의(P =0.003).수상도분석결과현시,TET2(rs7679673,A)화LMTK2(rs6465657,T)기인위점지간거리경근,존재경강적기인-기인교호작용.MDR방법험증최가모형적검험평형정도위0.5120,교차험증일치성위100％(P=0.0270).결론 FAM84B기인가능여중국인전렬선암환병풍험화종류분기유관,TET2、LMTK2화FAM84B기인적협동작용가능여전렬선암적환병풍험유관.
Objective To explore the association between the common variations of TET2 (rs7679673,A),MTK2 (rs6465657,T) and FAM84B (rs12543663,C) genes and prostate cancer (Pca) risk in Chinese population in Beijing,and to understand the relationship between genotypes and phenotypes including clinical characteristics and life style,etc.in patients with prostate cancer.Methods Based on a case-control study,124 patients with prostate cancer and 138 age-matched control subjects were recruited.Information of clinical phenotype and life style,etc.in the prostate cancer patients was collected.We compared the differences of allele and genotype frequencies of TET2 (rs7679673,A),LMTK2 (rs6465657,T) and FAM84B (rs12543663,C) gene expressions between the two groups for the allele and genotype frequencies,and explored the relationship between different genotypes and clinical features such as patient age,BMI,Gleason score,PSA level and tumor stage,by Chi-square test in patients with PCa.Multifactor dimensionality reduction was used to detect the gene-gene interactions.Results The FAM84B (rs12543663,C) C carriers frequency had significant difference between the case group and the control group (x2 =3.980 P =0.046 ; OR =1.883 ; 95％ CI =1.006-3.526).The allele and genotype frequencies of TET2 gene (rs7679673,A) and LMTK2 gene (rs6465657,T) were not significantly different between the case group and the control group (P ＞ 0.05).Analysis of the genotypes and clinical phenotypes showed that the genetic type of FAM84B C carriers [CX (CC + CA)] were significantly associated with cancer stage (x2 =9.585; P=0.002; OR =3.740; 95％ CI =1.580-8.853).Association between three loci and 12 kind of relevant outcomes was found in TET2 A carriers and the smoking and drinking patients (all P ＜0.05).Significant correlation was also found between LMTK2 (rs6465657,T) TX carriers and surgery (x2 =8.612; P =0.003; OR =0.174; 95％ CI 0.049-0.620).No significant correlation was seen with other covariates (P ＞ 0.05).Dendrogram analysis among the three loci showed that the best model consisted of the three sites (P =0.0270),cross validation consistency:10/10,and testing balanced accuracy:0.5120.There may be gene-gene interaction among TET2 (rs7679673,A),LMTK2 (rs6465657,T),and FAM84B (rs12543663,C).Conclusions There may be obvious association of FAM84B (rs12543663,C) gene with prostate cancer risk and the stages,and the synergistic effects of TET2 (rs7679673,A),LMTK2 (rs6465657,T) and FAM84B (rs12543663,C) genes may have an association with prostate cancer risk in Chinese population.
