中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2013年
4期
311-314
,共4页
王梦菡%常江%于典科%谭文%林东昕
王夢菡%常江%于典科%譚文%林東昕
왕몽함%상강%우전과%담문%림동흔
胃肿瘤%基因,ADD1%单核苷酸多态%易感性%磷酸化
胃腫瘤%基因,ADD1%單覈苷痠多態%易感性%燐痠化
위종류%기인,ADD1%단핵감산다태%역감성%린산화
Stomach neoplasms%Genes,ADD1%Single nucleotide polymorphism%Susceptibility%Phosphorylation
目的 探讨ADD1磷酸化位点错义突变与中国人群非贲门胃癌的易感性.方法 结合蛋白磷酸化位点数据库(PhosphoSitePlus)和SNP数据库(dbSNP),筛选出ADD1基因磷酸化位点错义突变,通过TaqMan分型方法检测1998例非贲门胃癌病例和2008例正常对照中错义突变的基因型.采用Logistic回归模型分析各基因型患者非贲门胃癌的发病风险.结果 找到了位于ADD1第586位磷酸化位点密码子上的错义突变rs4963.对照组中CC、CG和GG基因型频率分别为25.2％、50.4％和24.4％,病例组分别为20.1％、50.6％和29.3％,两组差异有统计学意义(P ＜0.001).与rs4963 CC基因型相比,CG基因型和GG基因型非贲门胃癌的发病风险显著升高(OR=1.24,95％ CI为1.06 ～ 1.46,P=0.008；OR=1.49,95％ CI为1.25～ 1.78,P＜0.001).结论 ADD1基因的磷酸化位点错义突变rs4963可能是非贲门胃癌的遗传易感因素.
目的 探討ADD1燐痠化位點錯義突變與中國人群非賁門胃癌的易感性.方法 結閤蛋白燐痠化位點數據庫(PhosphoSitePlus)和SNP數據庫(dbSNP),篩選齣ADD1基因燐痠化位點錯義突變,通過TaqMan分型方法檢測1998例非賁門胃癌病例和2008例正常對照中錯義突變的基因型.採用Logistic迴歸模型分析各基因型患者非賁門胃癌的髮病風險.結果 找到瞭位于ADD1第586位燐痠化位點密碼子上的錯義突變rs4963.對照組中CC、CG和GG基因型頻率分彆為25.2％、50.4％和24.4％,病例組分彆為20.1％、50.6％和29.3％,兩組差異有統計學意義(P ＜0.001).與rs4963 CC基因型相比,CG基因型和GG基因型非賁門胃癌的髮病風險顯著升高(OR=1.24,95％ CI為1.06 ～ 1.46,P=0.008；OR=1.49,95％ CI為1.25～ 1.78,P＜0.001).結論 ADD1基因的燐痠化位點錯義突變rs4963可能是非賁門胃癌的遺傳易感因素.
목적 탐토ADD1린산화위점착의돌변여중국인군비분문위암적역감성.방법 결합단백린산화위점수거고(PhosphoSitePlus)화SNP수거고(dbSNP),사선출ADD1기인린산화위점착의돌변,통과TaqMan분형방법검측1998례비분문위암병례화2008례정상대조중착의돌변적기인형.채용Logistic회귀모형분석각기인형환자비분문위암적발병풍험.결과 조도료위우ADD1제586위린산화위점밀마자상적착의돌변rs4963.대조조중CC、CG화GG기인형빈솔분별위25.2％、50.4％화24.4％,병례조분별위20.1％、50.6％화29.3％,량조차이유통계학의의(P ＜0.001).여rs4963 CC기인형상비,CG기인형화GG기인형비분문위암적발병풍험현저승고(OR=1.24,95％ CI위1.06 ～ 1.46,P=0.008；OR=1.49,95％ CI위1.25～ 1.78,P＜0.001).결론 ADD1기인적린산화위점착의돌변rs4963가능시비분문위암적유전역감인소.
Objective This study investigated the association between a missense SNP in the codon of ADD1 phosphorylation site and the susceptibility of non-cardia gastric cancer in a Chinese population.Methods PhosphoSitePlus and dbSNP database were combined to discover missense SNPs in the codon of phosphorylation site.Then,we genotyped the missense SNP in 1,998 cases with non-cardia gastric cancer and 2,008 cancer-free controls of Chinese descent.Analysis was conducted by using Logistic model adjusted by gender and age.Results The rs4963 in the codon of ADD1 phosphorylation site was found.The frequencies of the 3 rs4963 genotypes,CC,CG,GG,among controls were 25.2％,50.4％,and 24.4％,respectively,among patients were 20.1％,50.6％,and 29.3％,respectively.Compared with CC genotype,the rs4963 CG genotype and GG genotype significantly increased the risk of non-cardia gastric cancer with the odds ratios being 1.24 (95％ CI:1.06 ～ 1.46,P =0.008) and 1.49 (95％ CI:1.25 ～1.78,P ＜ 0.001),respectively.Conclusions Fnnctional polymorphism in the phosphorylation site of ADD1 (rs4963) may influence the susceptibility of non-cardia gastric cancer.
