中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2013年
5期
351-355
,共5页
涂小煌%黄少雄%李文生%宋京翔
塗小煌%黃少雄%李文生%宋京翔
도소황%황소웅%리문생%송경상
结直肠肿瘤%胱硫醚-β-合酶%甲基化%预后
結直腸腫瘤%胱硫醚-β-閤酶%甲基化%預後
결직장종류%광류미-β-합매%갑기화%예후
Colorectal neoplasms%Cystathionine-beta-synthase%Methylation%Prognosis
目的 探讨胱硫醚-β-合酶(cystathionine beta synthase,CBS)基因启动子区CpG岛的甲基化水平与结直肠癌患者临床病理特征的相关性.方法 通过结合重亚硫酸盐处理的直接测序、实时定量PCR和免疫组化等技术检测95例散发性结直肠癌患者肿瘤组织中CBS基因启动子区CpG岛的甲基化水平,分析其与结直肠癌患者临床病理特征之间的相关性及其对复发率和生存率的影响.结果 结直肠癌患者肿瘤组织中CBS基因启动子区CpG岛的甲基化水平为(64.9±14.3)％,显著高于正常组织[(27.5±13.1)％,P＜0.001].低甲基化组患者的CBS mRNA水平(7.22±1.91)显著高于高甲基化组(2.78 ±1.12,P＜0.01).单因素分析显示,患者年龄、pT分期、pN分期、肝转移、pTNM分期和CBS基因启动子区CpG岛甲基化水平与结直肠癌患者的预后有关(均P＜0.05).多因素分析显示,CBS基因启动子区CpG岛甲基化水平和肝转移为影响结直肠癌患者预后的独立因素(均P＜0.05).结论 CBS基因启动子区CpG岛的甲基化水平不仅与结直肠癌的发生、发展密切相关,而且可以作为一个有效的肿瘤分子诊断标志物,在结直肠癌早期诊断、靶向治疗和预后判断方面有一定意义.
目的 探討胱硫醚-β-閤酶(cystathionine beta synthase,CBS)基因啟動子區CpG島的甲基化水平與結直腸癌患者臨床病理特徵的相關性.方法 通過結閤重亞硫痠鹽處理的直接測序、實時定量PCR和免疫組化等技術檢測95例散髮性結直腸癌患者腫瘤組織中CBS基因啟動子區CpG島的甲基化水平,分析其與結直腸癌患者臨床病理特徵之間的相關性及其對複髮率和生存率的影響.結果 結直腸癌患者腫瘤組織中CBS基因啟動子區CpG島的甲基化水平為(64.9±14.3)％,顯著高于正常組織[(27.5±13.1)％,P＜0.001].低甲基化組患者的CBS mRNA水平(7.22±1.91)顯著高于高甲基化組(2.78 ±1.12,P＜0.01).單因素分析顯示,患者年齡、pT分期、pN分期、肝轉移、pTNM分期和CBS基因啟動子區CpG島甲基化水平與結直腸癌患者的預後有關(均P＜0.05).多因素分析顯示,CBS基因啟動子區CpG島甲基化水平和肝轉移為影響結直腸癌患者預後的獨立因素(均P＜0.05).結論 CBS基因啟動子區CpG島的甲基化水平不僅與結直腸癌的髮生、髮展密切相關,而且可以作為一箇有效的腫瘤分子診斷標誌物,在結直腸癌早期診斷、靶嚮治療和預後判斷方麵有一定意義.
목적 탐토광류미-β-합매(cystathionine beta synthase,CBS)기인계동자구CpG도적갑기화수평여결직장암환자림상병리특정적상관성.방법 통과결합중아류산염처리적직접측서、실시정량PCR화면역조화등기술검측95례산발성결직장암환자종류조직중CBS기인계동자구CpG도적갑기화수평,분석기여결직장암환자림상병리특정지간적상관성급기대복발솔화생존솔적영향.결과 결직장암환자종류조직중CBS기인계동자구CpG도적갑기화수평위(64.9±14.3)％,현저고우정상조직[(27.5±13.1)％,P＜0.001].저갑기화조환자적CBS mRNA수평(7.22±1.91)현저고우고갑기화조(2.78 ±1.12,P＜0.01).단인소분석현시,환자년령、pT분기、pN분기、간전이、pTNM분기화CBS기인계동자구CpG도갑기화수평여결직장암환자적예후유관(균P＜0.05).다인소분석현시,CBS기인계동자구CpG도갑기화수평화간전이위영향결직장암환자예후적독립인소(균P＜0.05).결론 CBS기인계동자구CpG도적갑기화수평불부여결직장암적발생、발전밀절상관,이차가이작위일개유효적종류분자진단표지물,재결직장암조기진단、파향치료화예후판단방면유일정의의.
Objective To explore the association between methylation of cystathionine-beta synthase (CBS) promoter and clinicopathological features in colorectal cancer.Methods Bisulfate sequencing PCR,real-time RT-PCR,and immunohistochemistry were used to investigate the methylation of CpG island in CBS promoter of 95 sporadic colorectal cancers.Software SPSS PASW Statistics was used to analyze the data of the hypermethylation levels in the malignant tissues and the correlation with pathological parameters and clinical outcome.Results Methylation levels in tumor tissue of patients [(64.9 ±14.3) ％] with colorectal cancer were significantly higher than that in normal tissues [(27.5 ±13.1) ％,P <0.001].The CBS mRNA levels in the hypomethylation group (7.22± 1.91) were significantly higher than that in the hypermethylation group (2.78 ±1.12,P <0.01).Univariate analysis showed that age,pT stage,pN stage,liver metastases,pTNM stage,and CBS hypermethylation level significantly correlated with the survival and recurrence rates of colorectal cancer patients (All P <0.05).Multivariate analysis showed that CBS hypermethylation level and liver metastasis were independent factors significantly correlated with the recurrence rate and overall survival of the patients (All P <0.05).Conclusions Our study indicates that methylation of CpG island in CBS promoter is correlated with the occurrence and progression of colorectal cancer and plays a role in its tumorigenesis.It might serve as a useful marker for early diagnosis,targeted therapy and prediction of prognosis in colorectal cancer.
