CAJ | 학술논문

目的探讨去整合素-金属蛋白酶17 (ADAM17) mRNA和蛋白在食管鳞癌组织中的表达,及其与患者临床病理特征和预后的关系.方法采用半定量逆转录聚合酶链反应(RT-PCR)检测50例食管鳞癌组织及其对应的正常食管黏膜组织中ADAM17 mRNA的表达量,采用免疫组化SP法检测80例食管鳞癌及其对应的正常食管黏膜组织中ADAM17蛋白的表达水平,采用Log rank检验和Cox比例风险模型进行预后生存分析.结果 ADAM17 mRNA在食管鳞癌及其对应的正常食管黏膜组织中的表达量分别为0.937 ±0.241和0.225 ±0.077,差异有统计学意义(P＜0.01).ADAM17蛋白在食管鳞癌及其对应的正常食管黏膜组织中的阳性表达率分别为66.2％ (53/80)和6.2％(5/80),差异有统计学意义(P＜0.01).ADAM17 mRNA和蛋白表达与食管鳞癌淋巴结转移和TNM分期有关(P＜0.05),与患者性别、年龄和组织学分级无关(P＞0.05).ADAM17蛋白表达与表皮生长因子受体(EGFR)蛋白表达呈正相关(P＜0.01).淋巴结转移、TNM分期、ADAM17和EGFR蛋白表达可作为影响食管鳞癌患者预后的独立指标(均P＜0.05).结论 ADAM17 mRNA和蛋白在食管鳞癌组织中高表达,其在食管鳞癌发生和侵袭转移中有重要作用,对预测食管鳞癌患者的预后有一定价值.
목적 탐토거정합소-금속단백매17 (ADAM17) mRNA화단백재식관린암조직중적표체,급기여환자림상병리특정화예후적관계.방법 채용반정량역전록취합매련반응(RT-PCR)검측50례식관린암조직급기대응적정상식관점막조직중ADAM17 mRNA적표체량,채용면역조화SP법검측80례식관린암급기대응적정상식관점막조직중ADAM17단백적표체수평,채용Log rank검험화Cox비례풍험모형진행예후생존분석.결과 ADAM17 mRNA재식관린암급기대응적정상식관점막조직중적표체량분별위0.937 ±0.241화0.225 ±0.077,차이유통계학의의(P＜0.01).ADAM17단백재식관린암급기대응적정상식관점막조직중적양성표체솔분별위66.2％ (53/80)화6.2％(5/80),차이유통계학의의(P＜0.01).ADAM17 mRNA화단백표체여식관린암림파결전이화TNM분기유관(P＜0.05),여환자성별、년령화조직학분급무관(P＞0.05).ADAM17단백표체여표피생장인자수체(EGFR)단백표체정정상관(P＜0.01).림파결전이、TNM분기、ADAM17화EGFR단백표체가작위영향식관린암환자예후적독립지표(균P＜0.05).결론 ADAM17 mRNA화단백재식관린암조직중고표체,기재식관린암발생화침습전이중유중요작용,대예측식관린암환자적예후유일정개치.
Objective To investigate the expression of A disintegrin and metalloproteinase 17 (ADAM17) mRNA and ADAM17 protein in esophageal squamous cell carcinoma (ESCC),and to evaluate their correlation with clinicopathological features and prognosis.Methods The expression of ADAM17 mRNA in 50 ESCC and 50 normal esophageal tissues was detected by RT-PCR.The expression of ADAM17 protein in 80 ESCC and 80 normal esophageal tissues was detected with immunohistochemioal staining (SP).Log rank test and Cox proportional hazards analysis were used to analyze the prognosis of ESCC.Results The expression of ADAM17 mRNA in 50 ESCC and 50 normal esophageal tissues was 0.937 ± 0.241 and 0.225 ±0.077,respectively.The positive expression rates of ADAM17 protein in 80 ESCC and 80 normal esophageal tissues was 66.2％ and 6.2％,respectively.The expressions of ADAM17 mRNA and ADAM17 protein in the ESCC were significantly higher than those in normal esophageal tissues (P ＜ 0.01).The expressions of ADAM17 mRNA and protein were positively correlated with lymph node metastasis and TNM staging (P ＜ 0.05).There were no correlations between the expressions of ADAM17 mRNA and protein and sex,age and histological grade(P ＞ 0.05).The expression of ADAM17 protein was positively correlated with EGFR protein (P ＜ 0.01).The lymph node metastasis,TNM staging and the expression of ADAM17 and EGFR protein were independent prognostic factors.Conclusions ADAM17 mRNA and protein are highly expressed in ESCC than in normal esophageal tissues and may play an important role in the development,invasion and metastasis of ESCC.They may be used as prognostic factors of ESCC.