CAJ | 학술논문

目的研究糖原合成激酶3(GSK-3)活性形式pGSK-3α/β (Tyr279/216)及其下游分子X连锁凋亡抑制蛋白(XIAP)在胆管癌组织中的表达情况,并探讨其表达与胆管癌临床病理特征及预后的关系.方法采用免疫组化法检测50例胆管癌组织和20例正常胆管组织中pGSK-3α/β(Tyr279/216)和XIAP蛋白的表达水平.结果 pGSK-3α/β (Tyr279/216)和XIAP蛋白在胆管癌组织中阳性表达率分别为62.0％和68.0％,在正常胆管组织中的阳性表达率分别为10.0％和25.0％,pGSK-3α/β(Tyr279/216)和XIAP蛋白在胆管癌组织中的表达强度显著高于正常胆管组织(均P＜0.001).Spearman相关分析显示,pGSK-3α/β (Tyr279/216)和XIAP蛋白在胆管癌组织中的表达具有显著相关性(r =0.544,P＜0.001).pGSK-3α/β (Tyr279/216)蛋白表达与胆管癌的TNM分期(P:0.042)和组织分化程度(P=0.031)有关,XIAP蛋白表达与血清CEA水平有关(P =0.006).pGSK3α/β (Tyr279/216)阳性表达和阴性表达患者的5年生存率分别为3.2％和26.3％ (P =0.002),XIAP阳性表达和阴性表达患者的5年生存率分别为5.9％和25.9％ (P =0.018).多因素分析结果显示,TNM分期(P =0.006)、术前总胆红素(P =0.001)、并发症(P =0.003)和pGSK-3∥β蛋白水平(P =0.002)是影响胆管癌患者预后的独立因素.结论 pGSK-3α/β (Tyr279/216)和XIAP蛋白与胆管癌的发生、发展有密切关系,pGSK-3α/β (Tyr279/216)可作为预测胆管癌预后的重要因素.
목적 연구당원합성격매3(GSK-3)활성형식pGSK-3α/β (Tyr279/216)급기하유분자X련쇄조망억제단백(XIAP)재담관암조직중적표체정황,병탐토기표체여담관암림상병리특정급예후적관계.방법 채용면역조화법검측50례담관암조직화20례정상담관조직중pGSK-3α/β(Tyr279/216)화XIAP단백적표체수평.결과 pGSK-3α/β (Tyr279/216)화XIAP단백재담관암조직중양성표체솔분별위62.0％화68.0％,재정상담관조직중적양성표체솔분별위10.0％화25.0％,pGSK-3α/β(Tyr279/216)화XIAP단백재담관암조직중적표체강도현저고우정상담관조직(균P＜0.001).Spearman상관분석현시,pGSK-3α/β (Tyr279/216)화XIAP단백재담관암조직중적표체구유현저상관성(r =0.544,P＜0.001).pGSK-3α/β (Tyr279/216)단백표체여담관암적TNM분기(P:0.042)화조직분화정도(P=0.031)유관,XIAP단백표체여혈청CEA수평유관(P =0.006).pGSK3α/β (Tyr279/216)양성표체화음성표체환자적5년생존솔분별위3.2％화26.3％ (P =0.002),XIAP양성표체화음성표체환자적5년생존솔분별위5.9％화25.9％ (P =0.018).다인소분석결과현시,TNM분기(P =0.006)、술전총담홍소(P =0.001)、병발증(P =0.003)화pGSK-3∥β단백수평(P =0.002)시영향담관암환자예후적독립인소.결론 pGSK-3α/β (Tyr279/216)화XIAP단백여담관암적발생、발전유밀절관계,pGSK-3α/β (Tyr279/216)가작위예측담관암예후적중요인소.
Objective To investigate the expressions of the active form of glycogen synthase kinase3 (GSK-3)-pGSK-3α/β (Tyr279/216) and its downstream moleculor X-linked inhibitor of apoptosis protein (XIAP) in cholangiocarcinoma and to analyze their correlation with clinicopathological and survival significance.Methods Immunohistoehemistry was used to detect the expressions of the active form of GSK3-pGSK-3α/ β (Tyr279/216) and its downstream moleculor XIAP proteins in 50 cholangiocarcinoma tissues and 20 normal bile duct tissues.Results The positive rates of pGSK-3α/β (Tyr279/216) and XIAP were 62.0％ and 68.0％ in cholangiocarcinoma,and 10.0％ and 25.0％ in normal bile duct tissues,respectively.The intensity of pGSK-3α/β (Tyr279/216) and XIAP expressions in cholangiocarcinoma were significantly higher than that in the normal bile duct tissues (P ＜ 0.001),and there was a significant correlation between pGSK-3α/β (Tyr279/216) and XIAP expressions (r =0.544,P ＜ 0.001).The expression of pGSK-3 α/β (Tyr279/216) protein in cholangiocarcinoma was associated with TNM stage (P =0.042),histological grade (P =0.031),whereas the expression of XIAP protein in cholangiocarcinoma was correlated with CEA level (P =0.006).Patients with positive expression of pGSK-3α/β (Tyr279/216) and XIAP demonstrate a significantly worse prognosis than that of patients with negative expression of pGSK-3α/ β(Tyr279/216) and XIAP for overall survival (P =0.002,P =0.018).Multivariate survival analysis revealed that positive pGSK-3α/ β (Tyr279/216) expression provided significant independent prognostic value for overall survival (P =0.002).Conclusions The expressions of pGSK-3α/β(Tyr279/216) and XIAP proteins were significantly associated with the development and progression of cholangiocarcinoma.pGSK-3α/β (Tyr279/216) may be an important prognostic factor for survival of patients with cholangiocarcinoma.