中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2013年
7期
486-490
,共5页
小鼠%细胞系,肿瘤%肿瘤消退,自行性%肿瘤转移
小鼠%細胞繫,腫瘤%腫瘤消退,自行性%腫瘤轉移
소서%세포계,종류%종류소퇴,자행성%종류전이
Mice%Cell line,tumor%Neoplasms regression,spontaneous%Neoplasms metastasis
目的 探讨不同品系小鼠瘤株跨系移植后移植瘤的生长转移情况,以及建立跨系小鼠肿瘤模型的可能性.方法 不同品系来源的H22、S180、U14、FC、Ca761、SMG-A和DCS小鼠瘤株分别接种于C57BL/6J、ICR或KM小鼠皮下,观察小鼠体内成瘤和转移情况,绘制生长曲线.结果 H22瘤株移植到C57BL/6J和ICR小鼠体内成瘤率均为100％.第17天时,C57BL/6J和ICR小鼠的瘤重分别为(2.8±0.4)g和(1.5±0.5)g,差异有统计学意义(P＜0.001).S180瘤株在C57BL/6J小鼠体内的成瘤率为100％,肿瘤稳定生长.U14瘤株接种于C57BL/6J和KM小鼠后第32天,C57BL/6J小鼠体内移植瘤瘤重[(10.2±2.2)g]低于KM小鼠[(12.6±3.4)g,P=0.002].接种U14瘤株的C57BL/6J和KM小鼠均出现肺和淋巴结转移.FC、Ca761和SMG-A瘤株接种于C57BL/6J小鼠后不能成瘤或成瘤后完全消退.DCS瘤株接种于C57BL/6J小鼠也有部分肿瘤发生消退,未消退的肿瘤在C57BL/6J小鼠体内连续传代,获得亚克隆DCS-C57瘤株,其在C57BL/6J小鼠稳定生长,成瘤率和肺转移率均为100％.结论 小鼠瘤株跨系体内移植后,分化差、恶性程度高的瘤株可成功建立移植瘤.免疫原性强的瘤株即使成瘤也会消退,无法跨系建立移植瘤.出现肿瘤部分消退的瘤株经连续传代后,可获得稳定的移植瘤模型.
目的 探討不同品繫小鼠瘤株跨繫移植後移植瘤的生長轉移情況,以及建立跨繫小鼠腫瘤模型的可能性.方法 不同品繫來源的H22、S180、U14、FC、Ca761、SMG-A和DCS小鼠瘤株分彆接種于C57BL/6J、ICR或KM小鼠皮下,觀察小鼠體內成瘤和轉移情況,繪製生長麯線.結果 H22瘤株移植到C57BL/6J和ICR小鼠體內成瘤率均為100％.第17天時,C57BL/6J和ICR小鼠的瘤重分彆為(2.8±0.4)g和(1.5±0.5)g,差異有統計學意義(P＜0.001).S180瘤株在C57BL/6J小鼠體內的成瘤率為100％,腫瘤穩定生長.U14瘤株接種于C57BL/6J和KM小鼠後第32天,C57BL/6J小鼠體內移植瘤瘤重[(10.2±2.2)g]低于KM小鼠[(12.6±3.4)g,P=0.002].接種U14瘤株的C57BL/6J和KM小鼠均齣現肺和淋巴結轉移.FC、Ca761和SMG-A瘤株接種于C57BL/6J小鼠後不能成瘤或成瘤後完全消退.DCS瘤株接種于C57BL/6J小鼠也有部分腫瘤髮生消退,未消退的腫瘤在C57BL/6J小鼠體內連續傳代,穫得亞剋隆DCS-C57瘤株,其在C57BL/6J小鼠穩定生長,成瘤率和肺轉移率均為100％.結論 小鼠瘤株跨繫體內移植後,分化差、噁性程度高的瘤株可成功建立移植瘤.免疫原性彊的瘤株即使成瘤也會消退,無法跨繫建立移植瘤.齣現腫瘤部分消退的瘤株經連續傳代後,可穫得穩定的移植瘤模型.
목적 탐토불동품계소서류주과계이식후이식류적생장전이정황,이급건립과계소서종류모형적가능성.방법 불동품계래원적H22、S180、U14、FC、Ca761、SMG-A화DCS소서류주분별접충우C57BL/6J、ICR혹KM소서피하,관찰소서체내성류화전이정황,회제생장곡선.결과 H22류주이식도C57BL/6J화ICR소서체내성류솔균위100％.제17천시,C57BL/6J화ICR소서적류중분별위(2.8±0.4)g화(1.5±0.5)g,차이유통계학의의(P＜0.001).S180류주재C57BL/6J소서체내적성류솔위100％,종류은정생장.U14류주접충우C57BL/6J화KM소서후제32천,C57BL/6J소서체내이식류류중[(10.2±2.2)g]저우KM소서[(12.6±3.4)g,P=0.002].접충U14류주적C57BL/6J화KM소서균출현폐화림파결전이.FC、Ca761화SMG-A류주접충우C57BL/6J소서후불능성류혹성류후완전소퇴.DCS류주접충우C57BL/6J소서야유부분종류발생소퇴,미소퇴적종류재C57BL/6J소서체내련속전대,획득아극륭DCS-C57류주,기재C57BL/6J소서은정생장,성류솔화폐전이솔균위100％.결론 소서류주과계체내이식후,분화차、악성정도고적류주가성공건립이식류.면역원성강적류주즉사성류야회소퇴,무법과계건립이식류.출현종류부분소퇴적류주경련속전대후,가획득은정적이식류모형.
Objective Mouse tumors were subcutaneously transplanted into different mouse strains and their growth and metastatic properties were checked,to explore the possibility of establishing animal tumor models in different mouse strains other than their normal host strains.Methods Seven mouse tumor cell lines:H22,S180,U14,FC,Ca761,SMG-A and DCS were transplanted into C57BL/6J,ICR or KM mice,and their tumorigenicity,growth and metastasis were recorded and analyzed.Results The tumor formation rate of H22 cells in both the C57BL/6J and ICR mice was 100％,but the growth of H22 tumors was significantly faster in the C57BL/6J (2.8 ±0.4)g than in the ICR mice (1.5 ±0.5)g at the 17th day after transplantation (P ＜0.001).The S180 tumors grew stably in C57BL/6J mice and the tumor formation rate was 100％.The U14 inoculated into C57BL/6J and KM mice showed both lymphatic and lung metastasis and formed significantly larger tumors in KM mice [(12.6 ± 3.4)g] than that in the C57BL/6J mice [(10.2 ±2.2)g] on the 32rd day after transplantation (P=0.002).Transplantation of FC,Ca761,and SMG-A did not form tumors or the tumors were completely regressed later in C57BL/6J mice.DCS cells formed tumors in C57BL/6J mice,but some of the tumors regressed.The retained tumors were passaged in C57BL/6J mice,and the substrain DCS-C57 cells was established which showed stable growth and had a 100％ tumor formation rate and 100％ lung metastasis rate in C57BL/6J mice.Conclusions Cross-strain transplanted tumors can be successfully established by inoculation of poorly differentiated and highly malignant tumor cells into different mouse strains.Some highly immunogenic tumor cells may form tumor,however,the tumors are regressed later,and can not establish cross-strain transplanted tumors in other mouse strains.Stable transplanted tumor models can be obtained from the partially regressed tumors after continuous passages in vivo.
