中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2013年
7期
547-551
,共5页
刘聪%王宇美%李哲轩%张联%马峻岭%周彤%游伟程%潘凯枫
劉聰%王宇美%李哲軒%張聯%馬峻嶺%週彤%遊偉程%潘凱楓
류총%왕우미%리철헌%장련%마준령%주동%유위정%반개풍
螺杆菌,幽门%抗体%胃肿瘤%癌前状态%免疫印迹法
螺桿菌,幽門%抗體%胃腫瘤%癌前狀態%免疫印跡法
라간균,유문%항체%위종류%암전상태%면역인적법
Helicobacter pylori%Antibodies%Stomach neoplasms%Precancerous conditions%Immunoblotting
目的 检测幽门螺杆菌(Hp)特异性抗体CagA、VacA、GroEL、UreA、HcpC和GGT在胃癌高发区人群中的分布,初步探讨其与胃黏膜病变的关系.方法 以1994-2003年、2002-2006年在山东省临朐县胃癌高发现场开展的2项干预队列研究为基础,选取623例经13C-尿素呼气试验(13 C-UBT)和(或)酶联免疫吸附试验(ELISA)检测为Hp感染阳性者为研究对象,采用基于基因重组的新型免疫印迹法recomLine方法对Hp的6种特异性抗体CagA、VacA、GroEL、UreA、HcpC和GGT进行血清学检测.结果 在623例研究对象中,Hp感染阳性者594例,其中CagA、VacA、GroEL、UreA、HcpC、GGT抗体阳性率分别为84.0％、38.2％、66.7％、17.7％、58.8％和42.8％；检出HpⅠ型感染者523例(88.1％).慢性萎缩性胃炎(CAG)组感染HpⅠ型患者所占的比例(89.7％)高于浅表性胃炎(SG)组(77.8％,P =0.001).结论 Hp不同毒力致病因子可能与胃黏膜病变程度相关.采用recomLine方法检测血清中的Hp特异性抗体,对于区分不同Hp菌株感染可能具有重要意义.
目的 檢測幽門螺桿菌(Hp)特異性抗體CagA、VacA、GroEL、UreA、HcpC和GGT在胃癌高髮區人群中的分佈,初步探討其與胃黏膜病變的關繫.方法 以1994-2003年、2002-2006年在山東省臨朐縣胃癌高髮現場開展的2項榦預隊列研究為基礎,選取623例經13C-尿素呼氣試驗(13 C-UBT)和(或)酶聯免疫吸附試驗(ELISA)檢測為Hp感染暘性者為研究對象,採用基于基因重組的新型免疫印跡法recomLine方法對Hp的6種特異性抗體CagA、VacA、GroEL、UreA、HcpC和GGT進行血清學檢測.結果 在623例研究對象中,Hp感染暘性者594例,其中CagA、VacA、GroEL、UreA、HcpC、GGT抗體暘性率分彆為84.0％、38.2％、66.7％、17.7％、58.8％和42.8％；檢齣HpⅠ型感染者523例(88.1％).慢性萎縮性胃炎(CAG)組感染HpⅠ型患者所佔的比例(89.7％)高于淺錶性胃炎(SG)組(77.8％,P =0.001).結論 Hp不同毒力緻病因子可能與胃黏膜病變程度相關.採用recomLine方法檢測血清中的Hp特異性抗體,對于區分不同Hp菌株感染可能具有重要意義.
목적 검측유문라간균(Hp)특이성항체CagA、VacA、GroEL、UreA、HcpC화GGT재위암고발구인군중적분포,초보탐토기여위점막병변적관계.방법 이1994-2003년、2002-2006년재산동성림구현위암고발현장개전적2항간예대렬연구위기출,선취623례경13C-뇨소호기시험(13 C-UBT)화(혹)매련면역흡부시험(ELISA)검측위Hp감염양성자위연구대상,채용기우기인중조적신형면역인적법recomLine방법대Hp적6충특이성항체CagA、VacA、GroEL、UreA、HcpC화GGT진행혈청학검측.결과 재623례연구대상중,Hp감염양성자594례,기중CagA、VacA、GroEL、UreA、HcpC、GGT항체양성솔분별위84.0％、38.2％、66.7％、17.7％、58.8％화42.8％；검출HpⅠ형감염자523례(88.1％).만성위축성위염(CAG)조감염HpⅠ형환자소점적비례(89.7％)고우천표성위염(SG)조(77.8％,P =0.001).결론 Hp불동독력치병인자가능여위점막병변정도상관.채용recomLine방법검측혈청중적Hp특이성항체,대우구분불동Hp균주감염가능구유중요의의.
Objective To determine the distributions of six Helicobacter pylori (Hp)-specific antibodies in a high-risk population of gastric cancer (GC) and explore the relationship between Hp virulence factors and precancerous gastric lesions.Methods Based on the two intervention trials conducted in Linqu County,the seropositivities for CagA,VacA,GroEL,UreA,HcpC and GGT were assessed by recombinant immunoassay (recomLine) in 623 participants with H.pylori infection determined by 13C-urea breath test (13 C-UBT) and/or enzyme linked immunosorbent assay (ELISA).Results In a total of 623 participants were detected by recomLine analysis,of which 594 were Hp-positive.The seropositivities rotes of CagA,VacA,GroEL,UreA,HcpC and GGT were 84.0％,38.2％,66.7％,17.7％,58.8％ and 42.8％,respectively.A total of 523 participants were determined as type Ⅰ infection of Hp,accounting for 88.1％.Compared with superficial gastritis (SG),the infection rate of Hp type Ⅰ was higher in the chronic atrophic gastritis (CAG) (P =0.001).Conclnsions The results of this population-based study suggest that the virulence factors of Hp may be related to the development of GC in a Chinese high-risk population.The recomLine analysis may serve as a tool for identification of Hp strains and prediction of high-risk population of GC.
