中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2013年
12期
910-913
,共4页
胥萍%陈慧%陈永井%陈延斌%顾国浩%吴妹英%吴敏娟%王雪峰%张学光
胥萍%陳慧%陳永井%陳延斌%顧國浩%吳妹英%吳敏娟%王雪峰%張學光
서평%진혜%진영정%진연빈%고국호%오매영%오민연%왕설봉%장학광
肺肿瘤%程序性死亡蛋白1%程序性死亡蛋白1受体%外周血%单个核细胞
肺腫瘤%程序性死亡蛋白1%程序性死亡蛋白1受體%外週血%單箇覈細胞
폐종류%정서성사망단백1%정서성사망단백1수체%외주혈%단개핵세포
Lung neoplasms%Programmed death protein-1%Programmed death protein-1 ligand%Peripheral blood%Mononuclear cells
目的 分析肺癌患者外周血中单个核细胞表面协同刺激分子程序性死亡蛋白1(PD-1)及其受体(PD-L1)的表达特性,并探讨其生物学意义.方法 取133例肺癌患者、25例肺部感染患者和23例健康志愿者的抗凝全血100μl,采用多色免疫荧光标记和流式细胞术对外周血中单个核细胞表面PD-1和PD-L1的表达水平进行检测和分析.结果 肺癌患者外周血中CD3+ CD8+T细胞亚群的比例为(38.83±1.74)％,明显低于健康志愿者[(43.25±3.35)％,P＜0.05].肺癌患者外周血中CD8+ CD28+T细胞亚群的比例为(17.73±1.21)％,明显低于健康志愿者[(27.96±2.72)％,P＜0.01].肺癌患者外周血中CD8+ CD28-T细胞亚群的比例为(21.19±1.92)％,明显高于健康志愿者[(15.18±2.93)％,P ＜0.05].肺癌患者外周血中CD8+ CD28+T细胞表面PD-1的表达水平为(10.67±1.12)％,较健康志愿者[(5.32±1.58)％]明显增高(P＜0.01).肺癌患者外周血中CD8+CD28-T细胞表面PD-1的表达水平为(7.46±1.25)％,明显高于健康志愿者[(2.68±1.07)％,P＜0.01].肺癌患者外周血中CD68+细胞表面PD-L1的表达水平为(16.03±2.06)％,较健康志愿者[(9.72±2.00)％]明显增高(P＜0.05).结论 PD-1和PD-L1在肺癌患者外周血中的表达上调对淋巴细胞产生负性调节作用,抑制了机体免疫系统对肿瘤细胞的杀伤效应,促进了肿瘤的发展并产生免疫逃逸.
目的 分析肺癌患者外週血中單箇覈細胞錶麵協同刺激分子程序性死亡蛋白1(PD-1)及其受體(PD-L1)的錶達特性,併探討其生物學意義.方法 取133例肺癌患者、25例肺部感染患者和23例健康誌願者的抗凝全血100μl,採用多色免疫熒光標記和流式細胞術對外週血中單箇覈細胞錶麵PD-1和PD-L1的錶達水平進行檢測和分析.結果 肺癌患者外週血中CD3+ CD8+T細胞亞群的比例為(38.83±1.74)％,明顯低于健康誌願者[(43.25±3.35)％,P＜0.05].肺癌患者外週血中CD8+ CD28+T細胞亞群的比例為(17.73±1.21)％,明顯低于健康誌願者[(27.96±2.72)％,P＜0.01].肺癌患者外週血中CD8+ CD28-T細胞亞群的比例為(21.19±1.92)％,明顯高于健康誌願者[(15.18±2.93)％,P ＜0.05].肺癌患者外週血中CD8+ CD28+T細胞錶麵PD-1的錶達水平為(10.67±1.12)％,較健康誌願者[(5.32±1.58)％]明顯增高(P＜0.01).肺癌患者外週血中CD8+CD28-T細胞錶麵PD-1的錶達水平為(7.46±1.25)％,明顯高于健康誌願者[(2.68±1.07)％,P＜0.01].肺癌患者外週血中CD68+細胞錶麵PD-L1的錶達水平為(16.03±2.06)％,較健康誌願者[(9.72±2.00)％]明顯增高(P＜0.05).結論 PD-1和PD-L1在肺癌患者外週血中的錶達上調對淋巴細胞產生負性調節作用,抑製瞭機體免疫繫統對腫瘤細胞的殺傷效應,促進瞭腫瘤的髮展併產生免疫逃逸.
목적 분석폐암환자외주혈중단개핵세포표면협동자격분자정서성사망단백1(PD-1)급기수체(PD-L1)적표체특성,병탐토기생물학의의.방법 취133례폐암환자、25례폐부감염환자화23례건강지원자적항응전혈100μl,채용다색면역형광표기화류식세포술대외주혈중단개핵세포표면PD-1화PD-L1적표체수평진행검측화분석.결과 폐암환자외주혈중CD3+ CD8+T세포아군적비례위(38.83±1.74)％,명현저우건강지원자[(43.25±3.35)％,P＜0.05].폐암환자외주혈중CD8+ CD28+T세포아군적비례위(17.73±1.21)％,명현저우건강지원자[(27.96±2.72)％,P＜0.01].폐암환자외주혈중CD8+ CD28-T세포아군적비례위(21.19±1.92)％,명현고우건강지원자[(15.18±2.93)％,P ＜0.05].폐암환자외주혈중CD8+ CD28+T세포표면PD-1적표체수평위(10.67±1.12)％,교건강지원자[(5.32±1.58)％]명현증고(P＜0.01).폐암환자외주혈중CD8+CD28-T세포표면PD-1적표체수평위(7.46±1.25)％,명현고우건강지원자[(2.68±1.07)％,P＜0.01].폐암환자외주혈중CD68+세포표면PD-L1적표체수평위(16.03±2.06)％,교건강지원자[(9.72±2.00)％]명현증고(P＜0.05).결론 PD-1화PD-L1재폐암환자외주혈중적표체상조대림파세포산생부성조절작용,억제료궤체면역계통대종류세포적살상효응,촉진료종류적발전병산생면역도일.
Objective To analyze the expression of co-stimutlatory molecules PD-1/PD-L1 in peripheral blood mononuclear cells in lung cancer patients,and to explore its biological significance.Methods One hundred and thirty-three lung cancer patients,25 lung infection patients and 23 healthy donors were enrolled in this study.100 μl of whole blood from these subjects were collected.Multi-color immunofluorescence staining and flow cytometry were used to detect PD-1/PD-L1 expression.The results were statistically analyzed.Results The expression level of CD3 + CD8 + T cells in the lung cancer patients was (38.83 ± 1.74) ％,significantly lower than that in the control group [(43.25 ± 3.35) ％,P ＜ 0.05].CD8 + CD28 + T cell subset in the peripheral blood of lung cancer patients was (17.73 ± 1.21)％ significantly lower than that of the healthy donors [(27.96 ±2.72) ％,P ＜0.01].The CD8 + CD28-T cell subset was (21.19 ± 1.92)％ in the lung cancer patients,significantly higher than that of the healthy control group [(15.18 ± 2.93) ％,P ＜ 0.05].The expression level of PD-1 on the surface of CD8 + CD28 +T cells was (10.67 ± 1.12) ％ in the group of lung cancer patients,significantly higher than that of the control group [(5.32 ± 1.58) ％,P ＜ 0.01].It was also found that the expression of PD-1 on CD8 +CD28-T cells was up-regulated in the group of lung cancer patients (7.46 ± 1.25) ％,significantly higher than that of the healthy control group [(2.68 + 1.07) ％,P ＜ 0.01].The expression level of PD-L1 on CD68 + cells in the lung cancer patients was (16.03 ±2.06) ％,significantly higher than that of the healthy control group [(9.32±2.00)％,P＜0.05].Conclusion Up-regulation of PD-1/PD-L1 on peripheral blood cells in lung cancer patients negatively regulates the lymphocytes,inhibits the immune response for killing tumor cells,and promotes tumor development and immune escape.
