CAJ | 학술논문

目的探讨CD133+直肠癌细胞对直肠癌放射线敏感性的影响.方法采用免疫磁珠分选法将直肠癌细胞株SW480分为CD133+和CD133-细胞,Western blot检测其CD133蛋白表达,克隆形成实验检测其克隆形成能力,annexin V-PI法检测放射线照射后细胞凋亡情况.分离、培养人原代直肠癌细胞,制备荷瘤裸鼠,免疫组化法检测肿瘤组织中CD133的表达情况,并观察放射线照射后荷瘤裸鼠的肿瘤生长情况.结果克隆形成实验显示,CD133+和CD133-直肠癌细胞的克隆形成率分别为(67.5±5.5)％和(5.1±3.3)％,CD133+直肠癌细胞的克隆形成能力显著强于CD133-细胞群(P＜0.01).经放射线照射后,CD133+和CD133-直肠癌细胞的凋亡率分别为(12.6±3.2)％和(38.8±6.7)％,差异有统计学意义(P＜0.01).荷瘤裸鼠肿瘤组织照射后第9天,CD133高表达照射组和CD133低表达照射组裸鼠的相对肿瘤体积分别为3.00±0.32和2.55±0.29,差异有统计学意义(P＜0.05).结论 CD133+直肠癌细胞群能显著提高直肠癌对放射线的耐受性,是增强直肠癌放疗敏感性的潜在靶点.
목적 탐토CD133+직장암세포대직장암방사선민감성적영향.방법 채용면역자주분선법장직장암세포주SW480분위CD133+화CD133-세포,Western blot검측기CD133단백표체,극륭형성실험검측기극륭형성능력,annexin V-PI법검측방사선조사후세포조망정황.분리、배양인원대직장암세포,제비하류라서,면역조화법검측종류조직중CD133적표체정황,병관찰방사선조사후하류라서적종류생장정황.결과 극륭형성실험현시,CD133+화CD133-직장암세포적극륭형성솔분별위(67.5±5.5)％화(5.1±3.3)％,CD133+직장암세포적극륭형성능력현저강우CD133-세포군(P＜0.01).경방사선조사후,CD133+화CD133-직장암세포적조망솔분별위(12.6±3.2)％화(38.8±6.7)％,차이유통계학의의(P＜0.01).하류라서종류조직조사후제9천,CD133고표체조사조화CD133저표체조사조라서적상대종류체적분별위3.00±0.32화2.55±0.29,차이유통계학의의(P＜0.05).결론 CD133+직장암세포군능현저제고직장암대방사선적내수성,시증강직장암방료민감성적잠재파점.
Objective To investigate the effect of CD133 + cells on radiosensitivity of rectal cancer cells.Methods In vitro experiments: CD133 + cells were purified with Immunomagnetic beads from human rectal cancer cell line SW480 cells and annexin V/PI staining was used to determine apoptosis in CD133 +and CD133-cells.In vivo experiments: Transplanted rectal tumor was established in 30 nude mice using primarily established SW480 cells.The tumor cells were divided into CD133-high and CD133-low groups based on the immunohistochemical staining of CD133 expression of the tumor cells.The tumor size after irradiation was recorded every three days.Results CD133 + cells had a much lower percentage of apoptosis after radiation exposure compared with CD133-cells [(12.6 ± 3.2) ％ vs.(38.8 ± 6.7) ％,P ＜ 0.01].In vivo experiment showed that the normalized tumor size of CD133-high group (3.00 ± 0.32) became significantly larger than that of the CD133-low group (2.55 ± 0.29) at the ninth day and this difference lasted until the observation end (P ＜ 0.05).Conclusions CD133 + cells have a radioresistant effect on rectal cancer cells and may become a potential therapeutic target in the radiotherapy of rectal cancer.