中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2014年
8期
571-574
,共4页
肺肿瘤%小鼠,裸%模型,动物%免疫组织化学%肿瘤移植
肺腫瘤%小鼠,裸%模型,動物%免疫組織化學%腫瘤移植
폐종류%소서,라%모형,동물%면역조직화학%종류이식
Lung neoplasms%Mice,nude%Models,animal%Immunohistochemistry%Neoplasm transplantation
目的 探讨裸鼠皮下及肾被膜下建立人肺癌移植瘤模型的可行性及差异.方法 将手术切除的11例肺癌组织标本分别种植在裸鼠的皮下及肾被膜下,观察其成瘤情况,病理切片观察移植瘤和原发瘤的一致性.采用抗人CD31单克隆抗体进行移植瘤切片的免疫组化染色,并鉴定第1代移植瘤的血供来源.结果 人肺癌组织均成功接种于裸鼠皮下及裸鼠肾被膜下,裸鼠皮下接种的平均操作时间为13 min,裸鼠肾被膜下接种的平均操作时间为45 min.裸鼠皮下组的成瘤率为36.4％(4/11),而裸鼠肾被膜下组的成瘤率为45.5％(5/11).HE染色显示,移植瘤组织形态与原发瘤保持一致性.CD31染色显示,83.3％(5/6)的移植瘤血管是人源性的.结论 采用裸鼠皮下接种人肺癌组织和肾被膜下接种人肺癌组织的方法均能建立人肺癌移植瘤模型.肾被膜下接种的成瘤率稍高,但操作复杂.皮下接种的成瘤率稍低,但操作简单,成瘤后易于观察.两种方法形成的第1代移植瘤与原肿瘤有高度的符合性,是一种接近人体的肺癌模型,可为肺癌研究提供良好的实验平台.
目的 探討裸鼠皮下及腎被膜下建立人肺癌移植瘤模型的可行性及差異.方法 將手術切除的11例肺癌組織標本分彆種植在裸鼠的皮下及腎被膜下,觀察其成瘤情況,病理切片觀察移植瘤和原髮瘤的一緻性.採用抗人CD31單剋隆抗體進行移植瘤切片的免疫組化染色,併鑒定第1代移植瘤的血供來源.結果 人肺癌組織均成功接種于裸鼠皮下及裸鼠腎被膜下,裸鼠皮下接種的平均操作時間為13 min,裸鼠腎被膜下接種的平均操作時間為45 min.裸鼠皮下組的成瘤率為36.4％(4/11),而裸鼠腎被膜下組的成瘤率為45.5％(5/11).HE染色顯示,移植瘤組織形態與原髮瘤保持一緻性.CD31染色顯示,83.3％(5/6)的移植瘤血管是人源性的.結論 採用裸鼠皮下接種人肺癌組織和腎被膜下接種人肺癌組織的方法均能建立人肺癌移植瘤模型.腎被膜下接種的成瘤率稍高,但操作複雜.皮下接種的成瘤率稍低,但操作簡單,成瘤後易于觀察.兩種方法形成的第1代移植瘤與原腫瘤有高度的符閤性,是一種接近人體的肺癌模型,可為肺癌研究提供良好的實驗平檯.
목적 탐토라서피하급신피막하건립인폐암이식류모형적가행성급차이.방법 장수술절제적11례폐암조직표본분별충식재라서적피하급신피막하,관찰기성류정황,병리절편관찰이식류화원발류적일치성.채용항인CD31단극륭항체진행이식류절편적면역조화염색,병감정제1대이식류적혈공래원.결과 인폐암조직균성공접충우라서피하급라서신피막하,라서피하접충적평균조작시간위13 min,라서신피막하접충적평균조작시간위45 min.라서피하조적성류솔위36.4％(4/11),이라서신피막하조적성류솔위45.5％(5/11).HE염색현시,이식류조직형태여원발류보지일치성.CD31염색현시,83.3％(5/6)적이식류혈관시인원성적.결론 채용라서피하접충인폐암조직화신피막하접충인폐암조직적방법균능건립인폐암이식류모형.신피막하접충적성류솔초고,단조작복잡.피하접충적성류솔초저,단조작간단,성류후역우관찰.량충방법형성적제1대이식류여원종류유고도적부합성,시일충접근인체적폐암모형,가위폐암연구제공량호적실험평태.
Objective The aim of this study was to establish patient-derived lung cancer xenograft models in nude mice by subcutaneous and subrenal capsule transplantation,and to analyze the differences in biological characteristics of the xenografts.Methods Surgically resected lung cancer specimens from 11 patients were implanted subcutaneously and under the renal capsule in nude mice.The tumor growth and histopathological features were observed and human origin of the blood vessels in the first-generation xenograft tumors was evaluated by SP immunohistochemistry using anti-human CD31 antibody.Results The patient-derived lung cancer tissues were successfully implanted subcutaneously and under the renal capsule in 11 nude mice.The operation time of subcutaneous implantation was 13 min,and the tumor formation rate was 36.4％ (4/11).The operation time of implantation under the renal capsule was 45 min,and the tumor formation rate was 45.5％ (5/11).Histopathological examination of the xenografts using HE staining showed the same morphology of the human lung cancers,and immunohistochemical observation with CD31staining showed that 83.3％ (5/6) blood vessels in the xenograft tumors was of human origin.Conelusions Both methods of subcutaneous and subrenal capsule transplantation can be used to successfully establish patientderived lung cancer xenograft models in nude mice.The subcutaneous implantation is simple to operate,less time-comsuming,and easy to observe the tumor growth,but with a lower success rate of tumor formation.Transplantation under the renal capsule has a higher tumor formation rate,but is more difficult to operate,taking more time,and difficult to observe the growth of the tumor.The xenograft tumors formed by both methods in the first generation display biological characteristics of human lung cancer,the xenograft tumor models are close to human lung cancer,and therefore may provide a stable,reliable,and useful animal model in human lung cancer research.
