中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2014年
8期
587-591
,共5页
癌,肝细胞%肝硬化%肝血管瘤%β-连环蛋白%肝细胞核因子1α%预后
癌,肝細胞%肝硬化%肝血管瘤%β-連環蛋白%肝細胞覈因子1α%預後
암,간세포%간경화%간혈관류%β-련배단백%간세포핵인자1α%예후
Carcinoma,hepatocellular%Liver cirrhosis%Hemangioma%β-catenin%Hepatocyte nuclear factor-1 α%Prognosis
目的 探讨β-连环蛋白(β-catenin)和肝细胞核因子1 α(HNF-1 α)在肝细胞癌(HCC)组织中的表达及其对预后的影响.方法 采用免疫组化方法检测50例HCC组织、50例癌旁肝硬化组织和7例肝血管瘤旁肝组织中β-catenin和HNF-1α的表达情况.结果 β-catenin在HCC组织、癌旁肝硬化组织和肝血管瘤旁肝组织中的异常表达率分别为74.0％ (37/50)、18.0％ (9/50)和14.3％(1/7),β-catenin在HCC组织中的异常表达率高于肝血管瘤旁肝组织(P =0.002)和癌旁肝硬化组织(P＜0.001),且β-catenin异常表达患者的预后差.在50例HCC组织中,HNF-1α阴性10例(20.0％),HNF-1α弱阳性20例(40.0％),HNF-1α中度阳性13例(26.0％),HNF-1α强阳性7例(14.0％).在50例癌旁肝硬化组织中,HNF-1α阴性6例(12.0％),HNF-1α弱阳性10例(20.0％),HNF-1α中度阳性26例(52.0％),HNF-1α强阳性8例(16.0％).在7例肝血管瘤旁肝组织中,HNF-1α弱阳性1例(14.3％),HNF-1α中度阳性2例(28.6％),HNF-1α强阳性4例(57.1％).HNF-1α在HCC组织中的表达显著低于癌旁肝硬化组织(P=0.008)和肝血管瘤旁肝组织(P=0.029),HNF-1α阳性表达患者的预后较好.β-catenin异常表达与HNF-1α表达强度呈负相关(r=-0.673,P＜0.001).结论 HCC的发生、发展与β-catenin的异常表达有关.在HCC中,Wnt/β-catenin信号通路与HNF-1α的表达有一定的关系.
目的 探討β-連環蛋白(β-catenin)和肝細胞覈因子1 α(HNF-1 α)在肝細胞癌(HCC)組織中的錶達及其對預後的影響.方法 採用免疫組化方法檢測50例HCC組織、50例癌徬肝硬化組織和7例肝血管瘤徬肝組織中β-catenin和HNF-1α的錶達情況.結果 β-catenin在HCC組織、癌徬肝硬化組織和肝血管瘤徬肝組織中的異常錶達率分彆為74.0％ (37/50)、18.0％ (9/50)和14.3％(1/7),β-catenin在HCC組織中的異常錶達率高于肝血管瘤徬肝組織(P =0.002)和癌徬肝硬化組織(P＜0.001),且β-catenin異常錶達患者的預後差.在50例HCC組織中,HNF-1α陰性10例(20.0％),HNF-1α弱暘性20例(40.0％),HNF-1α中度暘性13例(26.0％),HNF-1α彊暘性7例(14.0％).在50例癌徬肝硬化組織中,HNF-1α陰性6例(12.0％),HNF-1α弱暘性10例(20.0％),HNF-1α中度暘性26例(52.0％),HNF-1α彊暘性8例(16.0％).在7例肝血管瘤徬肝組織中,HNF-1α弱暘性1例(14.3％),HNF-1α中度暘性2例(28.6％),HNF-1α彊暘性4例(57.1％).HNF-1α在HCC組織中的錶達顯著低于癌徬肝硬化組織(P=0.008)和肝血管瘤徬肝組織(P=0.029),HNF-1α暘性錶達患者的預後較好.β-catenin異常錶達與HNF-1α錶達彊度呈負相關(r=-0.673,P＜0.001).結論 HCC的髮生、髮展與β-catenin的異常錶達有關.在HCC中,Wnt/β-catenin信號通路與HNF-1α的錶達有一定的關繫.
목적 탐토β-련배단백(β-catenin)화간세포핵인자1 α(HNF-1 α)재간세포암(HCC)조직중적표체급기대예후적영향.방법 채용면역조화방법검측50례HCC조직、50례암방간경화조직화7례간혈관류방간조직중β-catenin화HNF-1α적표체정황.결과 β-catenin재HCC조직、암방간경화조직화간혈관류방간조직중적이상표체솔분별위74.0％ (37/50)、18.0％ (9/50)화14.3％(1/7),β-catenin재HCC조직중적이상표체솔고우간혈관류방간조직(P =0.002)화암방간경화조직(P＜0.001),차β-catenin이상표체환자적예후차.재50례HCC조직중,HNF-1α음성10례(20.0％),HNF-1α약양성20례(40.0％),HNF-1α중도양성13례(26.0％),HNF-1α강양성7례(14.0％).재50례암방간경화조직중,HNF-1α음성6례(12.0％),HNF-1α약양성10례(20.0％),HNF-1α중도양성26례(52.0％),HNF-1α강양성8례(16.0％).재7례간혈관류방간조직중,HNF-1α약양성1례(14.3％),HNF-1α중도양성2례(28.6％),HNF-1α강양성4례(57.1％).HNF-1α재HCC조직중적표체현저저우암방간경화조직(P=0.008)화간혈관류방간조직(P=0.029),HNF-1α양성표체환자적예후교호.β-catenin이상표체여HNF-1α표체강도정부상관(r=-0.673,P＜0.001).결론 HCC적발생、발전여β-catenin적이상표체유관.재HCC중,Wnt/β-catenin신호통로여HNF-1α적표체유일정적관계.
Objective To study the expressions of β-catenin in hepatocellular carcinoma (HCC) tissue,adjacent cirrhotic liver tissue and hemangioma-surrounding liver tissue to understand whether their difference in expression will influence on the prognosis and to study the relationship between Wnt/β-catenin signaling pathway and HNF-1α expression.Methods 50 specimens of HCC,50 specimens of adjacent cirrhotic liver tissue and 7 specimens of hemangioma-surrounding liver tissue were used to detect the differences in the expression of β-catenin and HNF-1α in them by immunohistochemistry.Results The expression rate of β-catenin was 74.0％ (37/50) in the HCC tissue,18.0％ (9/50) in cirrhotic liver tissue,and 14.3％ (1/7) in hemangioma-surrounding liver tissue.The expression rate of β-catenin in HCC tissue was significantly higher than that in the hemangioma-surrounding liver tissue (P =0.002) and cirrhotic liver tissue (P ＜0.001).The patients with abnormal expression had worse prognosis.Among the 50 HCC cases,the expression of HNF-1α was negative in 20.0％ (10/50),weak positive in 40.0％ (20/50),moderately positive in 26.0％ (13/50),and strong positive in 14.0％ (7/50).Among the 50 adjacent cirrhotic liver tissues,the expression of HNF-1α was negative in 12.0％ (6/50),weak positive in 20.0％ (10/50),moderately positive in 52.0％ (26/50) and strong positive in 16.0％ (8/50).In the 7 cases of hemangiora-surrounding liver tissue,the expression of HNF-1α was negative in 0 (0/7),weak positive in 14.3％ (1/7),moderately positive in 28.6％ (2/7) and strong positive in 57.1％ (4/7).The positive expression rate of HNF-1α in the HCC tissue was significantly lower than that in the hemangioma-surrounding liver tissues(P =0.029) and adjacent cirrhotic liver tissues (P =0.008).The patients with positive HNF-1o expression had a better prognosis.The abnormal expression of β-catenin was negatively correlated with positive HNF-1 α expression (r =-0.673,P ＜ 0.001).Conclusions The occurrence and development of HCC is related to the abnormal β-catenin expression.There is a negative correlation between Wnt/β-catenin signaling pathway and HNF-1α expression.
