中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2014年
10期
761-765
,共5页
高巍%刘尚梅%鲁海珍%梁晶%王明荣%徐昕
高巍%劉尚梅%魯海珍%樑晶%王明榮%徐昕
고외%류상매%로해진%량정%왕명영%서흔
胃肿瘤%腺癌%非整倍性%原位杂交,荧光
胃腫瘤%腺癌%非整倍性%原位雜交,熒光
위종류%선암%비정배성%원위잡교,형광
Stomach neoplasms%Adenocarcinoma%Aneuploidy%In situ hybridization,fluorescence
目的 探讨11和20号染色体非整倍体在胃腺癌早期病理诊断和预后监测中的意义.方法 收集49例胃腺癌肿瘤、癌旁黏膜、距肿瘤远端切缘处黏膜组织进行11和20号染色体荧光原位杂交检测,分析11和20号染色体非整倍体与胃腺癌临床病理特征的关系.结果 胃腺癌肿瘤、癌旁、距肿瘤远端切缘处黏膜组织中11号染色体的非整倍体发生率分别为83.7％、40.8％和16.3％,差异有统计学意义(P＜0.001);20号染色体的非整倍体发生率分别为87.8％、53.1％和16.3％,差异有统计学意义(P＜0.001).胃腺癌肿瘤组织中11号染色体非整倍体与Lauren分型和淋巴结转移均有关(均P ＜0.05).胃腺癌距肿瘤远端切缘处黏膜组织中11号染色体非整倍体与患者性别和大体分型均有关(均P ＜0.005).胃腺癌癌旁黏膜、距肿瘤远端切缘处黏膜组织学形态主要呈慢性炎症、肠上皮化生和不典型增生.结论 检测11和20号染色体倍体改变可能有助于胃腺癌的早期诊断,并可能成为提示术后复发的有效方法.
目的 探討11和20號染色體非整倍體在胃腺癌早期病理診斷和預後鑑測中的意義.方法 收集49例胃腺癌腫瘤、癌徬黏膜、距腫瘤遠耑切緣處黏膜組織進行11和20號染色體熒光原位雜交檢測,分析11和20號染色體非整倍體與胃腺癌臨床病理特徵的關繫.結果 胃腺癌腫瘤、癌徬、距腫瘤遠耑切緣處黏膜組織中11號染色體的非整倍體髮生率分彆為83.7％、40.8％和16.3％,差異有統計學意義(P＜0.001);20號染色體的非整倍體髮生率分彆為87.8％、53.1％和16.3％,差異有統計學意義(P＜0.001).胃腺癌腫瘤組織中11號染色體非整倍體與Lauren分型和淋巴結轉移均有關(均P ＜0.05).胃腺癌距腫瘤遠耑切緣處黏膜組織中11號染色體非整倍體與患者性彆和大體分型均有關(均P ＜0.005).胃腺癌癌徬黏膜、距腫瘤遠耑切緣處黏膜組織學形態主要呈慢性炎癥、腸上皮化生和不典型增生.結論 檢測11和20號染色體倍體改變可能有助于胃腺癌的早期診斷,併可能成為提示術後複髮的有效方法.
목적 탐토11화20호염색체비정배체재위선암조기병리진단화예후감측중적의의.방법 수집49례위선암종류、암방점막、거종류원단절연처점막조직진행11화20호염색체형광원위잡교검측,분석11화20호염색체비정배체여위선암림상병리특정적관계.결과 위선암종류、암방、거종류원단절연처점막조직중11호염색체적비정배체발생솔분별위83.7％、40.8％화16.3％,차이유통계학의의(P＜0.001);20호염색체적비정배체발생솔분별위87.8％、53.1％화16.3％,차이유통계학의의(P＜0.001).위선암종류조직중11호염색체비정배체여Lauren분형화림파결전이균유관(균P ＜0.05).위선암거종류원단절연처점막조직중11호염색체비정배체여환자성별화대체분형균유관(균P ＜0.005).위선암암방점막、거종류원단절연처점막조직학형태주요정만성염증、장상피화생화불전형증생.결론 검측11화20호염색체배체개변가능유조우위선암적조기진단,병가능성위제시술후복발적유효방법.
Objective The aim of this study was to find out the value of chromosome aneuploidy in early diagnosis and prediction of postoperative recurrence of gastric adenocarcinoma (GAC).Methods Tissue samples,including 49 GAC,pairing pericancerous mucosa and normal gastric mucosa from the distant cutting margin were use in this study.Two centromere probes,Cen1 1 and Cen20 specific for chromosomes 11 and 20 were analyzed by FISH.The clinicopathological data were summarized.Results The incidence of aneuploidy of chromosome 11 in the tumors,pericancerous mucosa and normal gastric mucosa from the distant cutting margin were 83.7％,40.8％,and 16.3％,respectively (P ＜ 0.001),and those of chromosome 20 were 87.8％,53.1％,and 16.3％,respectively (P ＜ 0.001).The aneuploidy of Cen 11 displayed a significant correlation with Lauren' s claasification,and lymph node metastasis (P ＜ 0.05 for both).The pericancerous mucosa and normal gastric mucosa from the distant cutting margin displayed mainly chronic inflammatory changes,intestinal metaplasia and dysplasia.Conclusions Aneuploidy of Cen1 1 and Cen20 in pericancerous mucosa may be used as a candidate marker for early diagnosis of gastric adenocarcinoma and may have a predictive value of postoperative recurrence.
