中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2009年
6期
451-455
,共5页
昝涛%董继英%李华%翁瑞%杨湄%谢芸%李青峰
昝濤%董繼英%李華%翁瑞%楊湄%謝蕓%李青峰
잠도%동계영%리화%옹서%양미%사예%리청봉
血管内皮祖细胞%血管内皮生长因子%外科皮瓣%新生血管化
血管內皮祖細胞%血管內皮生長因子%外科皮瓣%新生血管化
혈관내피조세포%혈관내피생장인자%외과피판%신생혈관화
Endothelial progenitor cells%Vascular endothelial growth factor%Surgical flaps%Neovascularization
目的 比较血管内皮祖细胞(endothelial progenitor cells,EPCs)与血管内皮生长因子(vascular endothelial growth factor,VEGF)在促进预构皮瓣血管新生作用上的差异,探讨EPCs移植提高预构皮瓣存活面积的可行性.方法 分离雄性Wistar大鼠(45只)一侧股血管柬,转位植入腹部皮下,建立预构皮瓣实验模型.将体外诱导分化的EPCs(组Ⅰ,n=15)和VEGF(组Ⅱ,n=15)分别注射于皮瓣局部,对照组仅注射PBS溶液(组Ⅲ,n=15).4周后形成以植入血管为蒂的岛状皮瓣,原位缝合;术后7 d对皮瓣存活率、血管密度计数进行检测.结果 组Ⅰ、组Ⅱ、组Ⅲ的皮瓣存活率分别为(87.26±10.13)%、(66.13±9.9)%、(55.59±13.06)%,组Ⅰ分别与组Ⅱ和组Ⅲ比较,差异均有统计学意义(P<0.001);微血管密度分别为:(38.67±9.52)个/mm~2、(25.83±6.33)个/mm~2、(26.5±5.61)个/mm~2(P<0.05).结论 EPCs促进预构皮瓣血管新生的作用优于VEGF,局部应用骨髓来源的EPCs可以有效地提高预构皮瓣存活面积.
目的 比較血管內皮祖細胞(endothelial progenitor cells,EPCs)與血管內皮生長因子(vascular endothelial growth factor,VEGF)在促進預構皮瓣血管新生作用上的差異,探討EPCs移植提高預構皮瓣存活麵積的可行性.方法 分離雄性Wistar大鼠(45隻)一側股血管柬,轉位植入腹部皮下,建立預構皮瓣實驗模型.將體外誘導分化的EPCs(組Ⅰ,n=15)和VEGF(組Ⅱ,n=15)分彆註射于皮瓣跼部,對照組僅註射PBS溶液(組Ⅲ,n=15).4週後形成以植入血管為蒂的島狀皮瓣,原位縫閤;術後7 d對皮瓣存活率、血管密度計數進行檢測.結果 組Ⅰ、組Ⅱ、組Ⅲ的皮瓣存活率分彆為(87.26±10.13)%、(66.13±9.9)%、(55.59±13.06)%,組Ⅰ分彆與組Ⅱ和組Ⅲ比較,差異均有統計學意義(P<0.001);微血管密度分彆為:(38.67±9.52)箇/mm~2、(25.83±6.33)箇/mm~2、(26.5±5.61)箇/mm~2(P<0.05).結論 EPCs促進預構皮瓣血管新生的作用優于VEGF,跼部應用骨髓來源的EPCs可以有效地提高預構皮瓣存活麵積.
목적 비교혈관내피조세포(endothelial progenitor cells,EPCs)여혈관내피생장인자(vascular endothelial growth factor,VEGF)재촉진예구피판혈관신생작용상적차이,탐토EPCs이식제고예구피판존활면적적가행성.방법 분리웅성Wistar대서(45지)일측고혈관간,전위식입복부피하,건립예구피판실험모형.장체외유도분화적EPCs(조Ⅰ,n=15)화VEGF(조Ⅱ,n=15)분별주사우피판국부,대조조부주사PBS용액(조Ⅲ,n=15).4주후형성이식입혈관위체적도상피판,원위봉합;술후7 d대피판존활솔、혈관밀도계수진행검측.결과 조Ⅰ、조Ⅱ、조Ⅲ적피판존활솔분별위(87.26±10.13)%、(66.13±9.9)%、(55.59±13.06)%,조Ⅰ분별여조Ⅱ화조Ⅲ비교,차이균유통계학의의(P<0.001);미혈관밀도분별위:(38.67±9.52)개/mm~2、(25.83±6.33)개/mm~2、(26.5±5.61)개/mm~2(P<0.05).결론 EPCs촉진예구피판혈관신생적작용우우VEGF,국부응용골수래원적EPCs가이유효지제고예구피판존활면적.
Objective To compare the effect of local administration of endothelial progenitor cells (EPCs) and VEGF on improving neovascularization and augmenting the survival areas in a rat model of prefabricated flap. Methods Prefabricated flaps were created by ligating the right femoral vascular pedicle and implanting it underneath the abdominal flap. The in vitro cultured EPCs(Group Ⅰ, n=15)and VEGF protein (Group Ⅱ, n=15)were injected subcutaneously around the implanted pedicle in experimental groups. PBS was injected in control group(Group Ⅲ, n=15). 4 weeks later, the abdominal island flap based solely on the implanted vessels was elevated and sutured back. Then flap viability and numbers of capillary were evaluated on day7. Results There was more statistically significant augmentation of flap survival [(87.26±10.13)% versus(66.13±9.9)% and(55.59±13.06)%, P<0.001], a higher capillary density(38.67±9.52 versus 25.83±6.33 and 26.5±5.61 capillary/mm~2, P<0.05)in EPCs group than in the other two groups.Conclusions EPCs are superior to VEGF in improving neovascularization during flap prefabrication. Local transplantation of bone marrow-derived EPCs may be a useful strategy for augmentation of the survival areas of prefabricated flaps.
