CAJ | 학술논문

目的探讨脂肪瘤吸除术终末质硬肿瘤组织的性质,及其残留体内在脂肪瘤吸除术后复发中的作用.方法采用脂肪瘤吸除术治疗多发性脂肪瘤患者,将获得的脂肪瘤吸除术终末质硬肿瘤组织标本进行HE染色以及干细胞标记抗体CD29、CD44免疫组化染色,以正常脂肪组织作为对照,行病理学分析；取平均阳性细胞密度和面积进行统计学分析.结果大体病理:脂肪瘤吸除术终末质硬肿瘤组织质地中等硬度,中央颜色微黄,周边散在灰白色质地疏松、不规则、条梭状膜样组织；正常脂肪组织颜色微黄,质地柔软均匀,无质硬结节存在.HE染色见脂肪瘤吸除术终末质硬肿瘤组织有较多的间质成分,间质中富有成纤维细胞样细胞,成簇分布于脂肪细胞之间,胞浆较少；正常脂肪组织,脂肪细胞呈圆形或多边形,且大小一致、形态均一、间质较少,脂肪细胞之间可见少量成纤维细胞样细胞.免疫组织化学染色发现,脂肪瘤吸除术终末质硬肿瘤组织有大量成纤维细胞样细胞被干细胞标记抗体CD29、CD44标记.2组标本阳性细胞密度和阳性细胞面积进行两独立样本均数的t ＇检验,分别为:t′D29(密度)=9.931,t′D44(密度)=10.171,t′CD29(面积)=7.761,t′CD44(面积)=6.639,P值均为0.000,差异有统计学意义.结论在脂肪瘤吸除术终末质硬肿瘤组织中,分布着大量被干细胞标记抗体标记的脂肪瘤间质干细胞；此终末质硬肿瘤组织残留,可能是引起脂肪瘤吸除术后复发的原因.
목적 탐토지방류흡제술종말질경종류조직적성질,급기잔류체내재지방류흡제술후복발중적작용.방법 채용지방류흡제술치료다발성지방류환자,장획득적지방류흡제술종말질경종류조직표본진행HE염색이급간세포표기항체CD29、CD44면역조화염색,이정상지방조직작위대조,행병이학분석；취평균양성세포밀도화면적진행통계학분석.결과 대체병리:지방류흡제술종말질경종류조직질지중등경도,중앙안색미황,주변산재회백색질지소송、불규칙、조사상막양조직；정상지방조직안색미황,질지유연균균,무질경결절존재.HE염색견지방류흡제술종말질경종류조직유교다적간질성분,간질중부유성섬유세포양세포,성족분포우지방세포지간,포장교소；정상지방조직,지방세포정원형혹다변형,차대소일치、형태균일、간질교소,지방세포지간가견소량성섬유세포양세포.면역조직화학염색발현,지방류흡제술종말질경종류조직유대량성섬유세포양세포피간세포표기항체CD29、CD44표기.2조표본양성세포밀도화양성세포면적진행량독립양본균수적t ＇검험,분별위:t′D29(밀도)=9.931,t′D44(밀도)=10.171,t′CD29(면적)=7.761,t′CD44(면적)=6.639,P치균위0.000,차이유통계학의의.결론 재지방류흡제술종말질경종류조직중,분포착대량피간세포표기항체표기적지방류간질간세포；차종말질경종류조직잔류,가능시인기지방류흡제술후복발적원인.
Objective To study the nature of the residue hard tissue after liposuction in lipoma and its role in lipoma recurrence.Methods The residue hard tissue after liposuction was collected for histologic study,including HE-staining and immunohistochemistry with antibody of stem cell marker(CD29 and CD44).The average density and area of positive cells were analyzed statistically.Results The residue hard tissue had a medium hardness.The center tissue was light yellow surrounding by soft,loose,irregular and cord-like tissue.The normal fat tissue is light yellow with a soft and unanimous texture.HEstaining showed abundant stromas in residue hard tissue,with lots of fibroblast-like cells locating among adipocytes.The cytoplasm was less with large and blue-staining nucleus.In normal fat tissue,the fat cells were uniform which were round or polygon.The stromas was less and the fibroblast-like cells were comparatively less.The slices were stained with antibody (CD29,CD44) of stem cell marker.The density and area of positive cells were much more in residue hard tissue than those in normal adipose tissue.Two Independence Samples T test were as follows:t′CD29 =9.931,P =0.000; t′CD44 =10.171,P =0.000 for density; and t′CD29 =7.761,P =0.000; tt′CD44 =6.639,P =0.000 for area.The difference was significant.Conclusions Abundant fibroblast-like cells that are signed by the marker of stem cells exist in residue hard tissue.Those cells are proven to be lipoma derived stem cells (LDSCs) which may play an role in lipoma recurrence after lipoma liposuction.