中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2013年
2期
117-120
,共4页
瘢痕疙瘩%DNA甲基转移酶1%成纤维细胞
瘢痕疙瘩%DNA甲基轉移酶1%成纖維細胞
반흔흘탑%DNA갑기전이매1%성섬유세포
Keloid%DNA methyltransferase 1%Fibroblasts
目的 探讨DNA甲基转移酶1(DNA methyltransferase 1,DNMT1)在瘢痕疙瘩的不同生长期和不同部位的成纤维细胞表达及其意义.方法 收集53例瘢痕疙瘩患者手术切除的瘢痕疙瘩标本,并按生长期分为A(＜6个月)、B(6 ～12个月)、C(1～2年)、D(＞2年)4组；免疫组织化学法检测各组标本正常皮肤、浸润部、增生部及老化部成纤维细胞DNMT1的表达并比较差异；RT-PCR法检测各组标本及不同部位成纤维细胞DNMT1mRNA的表达.结果 DNMT1在成纤维细胞中主要分布于细胞核内；瘢痕疙瘩成纤维细胞DNMT1蛋白阳性表达率(72％)及DNMT1mRNA表达均高于正常皮肤成纤维细胞(8％,P =0.001)；瘢痕疙瘩生长期较短组成纤维细胞DNMT1阳性表达率(100％,评分:10.47±1.85)及DNMT1mRNA表达均高于生长期较长组(45％,评分:7.71 +1.80,P =0.007)；瘢痕疙瘩浸润部(100％,评分:10.47±1.85)成纤维细胞DNMT1阳性表达率及DNMT1mRNA表达均高于增生部(78％,评分:6.63±1.75)与老化部(38％,评分:5.53 +1.55,P=0.001).结论 DNMTI在瘢痕疙瘩发病中具有重要作用；随着瘢痕疙瘩生长期的延长,DNMT1的表达有降低趋势；随着瘢痕疙瘩中央成纤维细胞老化,DNMT1表达降低.提示DNMT1在瘢痕疙瘩生长过程中起着重要的作用,并且与瘢痕疙瘩浸润生长密切相关.
目的 探討DNA甲基轉移酶1(DNA methyltransferase 1,DNMT1)在瘢痕疙瘩的不同生長期和不同部位的成纖維細胞錶達及其意義.方法 收集53例瘢痕疙瘩患者手術切除的瘢痕疙瘩標本,併按生長期分為A(＜6箇月)、B(6 ～12箇月)、C(1～2年)、D(＞2年)4組；免疫組織化學法檢測各組標本正常皮膚、浸潤部、增生部及老化部成纖維細胞DNMT1的錶達併比較差異；RT-PCR法檢測各組標本及不同部位成纖維細胞DNMT1mRNA的錶達.結果 DNMT1在成纖維細胞中主要分佈于細胞覈內；瘢痕疙瘩成纖維細胞DNMT1蛋白暘性錶達率(72％)及DNMT1mRNA錶達均高于正常皮膚成纖維細胞(8％,P =0.001)；瘢痕疙瘩生長期較短組成纖維細胞DNMT1暘性錶達率(100％,評分:10.47±1.85)及DNMT1mRNA錶達均高于生長期較長組(45％,評分:7.71 +1.80,P =0.007)；瘢痕疙瘩浸潤部(100％,評分:10.47±1.85)成纖維細胞DNMT1暘性錶達率及DNMT1mRNA錶達均高于增生部(78％,評分:6.63±1.75)與老化部(38％,評分:5.53 +1.55,P=0.001).結論 DNMTI在瘢痕疙瘩髮病中具有重要作用；隨著瘢痕疙瘩生長期的延長,DNMT1的錶達有降低趨勢；隨著瘢痕疙瘩中央成纖維細胞老化,DNMT1錶達降低.提示DNMT1在瘢痕疙瘩生長過程中起著重要的作用,併且與瘢痕疙瘩浸潤生長密切相關.
목적 탐토DNA갑기전이매1(DNA methyltransferase 1,DNMT1)재반흔흘탑적불동생장기화불동부위적성섬유세포표체급기의의.방법 수집53례반흔흘탑환자수술절제적반흔흘탑표본,병안생장기분위A(＜6개월)、B(6 ～12개월)、C(1～2년)、D(＞2년)4조；면역조직화학법검측각조표본정상피부、침윤부、증생부급노화부성섬유세포DNMT1적표체병비교차이；RT-PCR법검측각조표본급불동부위성섬유세포DNMT1mRNA적표체.결과 DNMT1재성섬유세포중주요분포우세포핵내；반흔흘탑성섬유세포DNMT1단백양성표체솔(72％)급DNMT1mRNA표체균고우정상피부성섬유세포(8％,P =0.001)；반흔흘탑생장기교단조성섬유세포DNMT1양성표체솔(100％,평분:10.47±1.85)급DNMT1mRNA표체균고우생장기교장조(45％,평분:7.71 +1.80,P =0.007)；반흔흘탑침윤부(100％,평분:10.47±1.85)성섬유세포DNMT1양성표체솔급DNMT1mRNA표체균고우증생부(78％,평분:6.63±1.75)여노화부(38％,평분:5.53 +1.55,P=0.001).결론 DNMTI재반흔흘탑발병중구유중요작용；수착반흔흘탑생장기적연장,DNMT1적표체유강저추세；수착반흔흘탑중앙성섬유세포노화,DNMT1표체강저.제시DNMT1재반흔흘탑생장과정중기착중요적작용,병차여반흔흘탑침윤생장밀절상관.
Objective To investigate the expression and significance of DNA methyltransferase 1 (DNMT1) in different parts and formation time of keloid fibroblasts.Methods Specimen were collected from fifty-three patients with keloid.The keloids were grouped according to formation time.Immunohistochemistry was used to assay the expression of DNMT1 in normal skin,invasive part,proliferative part and aged part.RT-PCR was adopted to assay the expression of DNMT1 mRNA in each group.Results DNMT1 is mainly distributed in the nucleus of fibroblasts.The expression of DNMT1protein and DNMT1mRNA in keloid (72％) is higher than that in normal skin fibroblasts (8％) (P =0.001).The expression rate of DNMT1 protein and DNMT1 mRNA in shorter formation time group (100％,Score:10.47 ± 1.85) is higher than a longer formation time group(45％,Score:7.71 + 1.80) (P=0.007).The expression rate of DNMT1 protein and DNMT1 mRNA in the invasive part of keloid (100％,Score:10.47 ± 1.85) is higher than the proliferative part (78％,Score:6.63 ± 1.75) and aged part(38％,Score:5.53 ±1.55) (P=0.001).Conclusions DNMT1 plays an important role in keloid.The expression of DNMT1 tends to decrease along with the increase of the formation time in keloid.It is also related to the part of keloid.DNMT1 plays an important role in the process of keloid growth and is closely related to infiltrative growth in keloid.
