中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2013年
2期
125-130
,共6页
骨生成,牵张%土鳖虫%骨形成蛋白%血管内皮生长因子
骨生成,牽張%土鱉蟲%骨形成蛋白%血管內皮生長因子
골생성,견장%토별충%골형성단백%혈관내피생장인자
Osteogenesis,distraction%Eupolyphaga Sinensis Walker%Bone morphogenetic proteins%Vascular endothelial growth factor
目的 研究单昧中药土鳖虫能否促进下颌骨牵张成骨过程中的骨形成.方法 将30只日本大耳白兔(性别不限,体重2.0～2.5 kg)随机分为实验组和对照组,每组各15只,均建立右侧兔下颌骨牵张成骨模型.实验组自牵张期第1天起每天喂食土鳖虫粉2 g,直至处死；对照组正常饮食.于固定期24、72 h,1、4和7周分别处死实验组和对照组大耳兔各3只,对标本进行大体观察、X线观察、组织学观察及骨形成蛋白(bone morphogenetic proteins,BMPs)、血管内皮生长因子(vascularendothelial growth factor,VEGF)免疫组织化学染色分析.结果 实验组动物牵张间隙区内的新骨形成速度明显快于对照组,同一时期实验组成骨活动较对照组活跃；实验组BMPs及VEGF的表达明 显高于对照组(P＜0.05).结论 中药土鳖虫可促进兔下颌骨牵张成骨过程中的骨形成,其作用机制可能与其促进BMPs及VEGF的表达有关.
目的 研究單昧中藥土鱉蟲能否促進下頜骨牽張成骨過程中的骨形成.方法 將30隻日本大耳白兔(性彆不限,體重2.0～2.5 kg)隨機分為實驗組和對照組,每組各15隻,均建立右側兔下頜骨牽張成骨模型.實驗組自牽張期第1天起每天餵食土鱉蟲粉2 g,直至處死；對照組正常飲食.于固定期24、72 h,1、4和7週分彆處死實驗組和對照組大耳兔各3隻,對標本進行大體觀察、X線觀察、組織學觀察及骨形成蛋白(bone morphogenetic proteins,BMPs)、血管內皮生長因子(vascularendothelial growth factor,VEGF)免疫組織化學染色分析.結果 實驗組動物牽張間隙區內的新骨形成速度明顯快于對照組,同一時期實驗組成骨活動較對照組活躍；實驗組BMPs及VEGF的錶達明 顯高于對照組(P＜0.05).結論 中藥土鱉蟲可促進兔下頜骨牽張成骨過程中的骨形成,其作用機製可能與其促進BMPs及VEGF的錶達有關.
목적 연구단매중약토별충능부촉진하합골견장성골과정중적골형성.방법 장30지일본대이백토(성별불한,체중2.0～2.5 kg)수궤분위실험조화대조조,매조각15지,균건립우측토하합골견장성골모형.실험조자견장기제1천기매천위식토별충분2 g,직지처사；대조조정상음식.우고정기24、72 h,1、4화7주분별처사실험조화대조조대이토각3지,대표본진행대체관찰、X선관찰、조직학관찰급골형성단백(bone morphogenetic proteins,BMPs)、혈관내피생장인자(vascularendothelial growth factor,VEGF)면역조직화학염색분석.결과 실험조동물견장간극구내적신골형성속도명현쾌우대조조,동일시기실험조성골활동교대조조활약；실험조BMPs급VEGF적표체명 현고우대조조(P＜0.05).결론 중약토별충가촉진토하합골견장성골과정중적골형성,기작용궤제가능여기촉진BMPs급VEGF적표체유관.
Objective To study the effect of Eupolyphaga Sinensis Walker on mandibular distraction osteogenesis (DO) in rabbits.Methods 30 Japanese white rabbits (weight 2.0-2.5 kg,about 3 months old) were divided randomly into control group (n =15) and experimental group (n =15).Unilateral mandibular DO models were established at the right mandible of the rabbits.Distraction was started 7 days after the surgery at the speed of 0.4 mm per time twice a day and continued for 10 days.From the first day of distraction to the day of execution,the experimental group rabbits were fed with 2 g of ESW power once a day at 9 o' clock.Three animals in each group were executed respectively at 24 hours,72 hours,1 week,4 weeks and 7 weeks after completion of distraction,and the specimens of DO were harvested.The general observation,X-ray examination,histological study and immunohistochemical staining of bone morphogenetic proteins (BMPs) and vascular endothelial growth factor (VEGF) were performed.The images of immuuohistochemical staining of BMPs and VEGF were analyzed by the image analysis software,and the results were analyzed by statistical software SPSS 17.0.Results The rate of the new bone formation in the experimental group was faster than that in the control group,and the immunohistochemical staining of BMPs and VEGF in the experimental group was higher than that in the control group.Conclusions ESW can promote the formation of the new bone in the distracted gap during mandibular DO in rabbits,which may be due to its enhancement effect on the expression of BMPs and VEGF.
