中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2013年
3期
192-196
,共5页
胡艺觉%胡志奇%冯传波%苗勇
鬍藝覺%鬍誌奇%馮傳波%苗勇
호예각%호지기%풍전파%묘용
真皮细胞%细胞移植%毛囊%小室法
真皮細胞%細胞移植%毛囊%小室法
진피세포%세포이식%모낭%소실법
Dermal cells%Cell transplantation%Hair follicle%Chamber assay
目的 探讨胚胎期真皮信号对体外扩增后的新生鼠真皮细胞毛发诱导能力的影响.方法 将胎龄14 d的胚鼠真皮细胞,加入到由体外扩增3d的新生鼠真皮细胞与新鲜分离的新生鼠表皮细胞在裸鼠背部构建的小室中,将其毛发再生情况与未添加胚鼠真皮细胞时做比较.同时,分别以下列细胞构建小室作为对照:胚鼠真皮细胞+新生鼠表皮细胞；新鲜分离的新生鼠真皮细胞+新生鼠表皮细胞；扩增的新生鼠真皮细胞；胚鼠真皮细胞；新鲜分离的新生鼠真皮细胞；新生鼠表皮细胞.结果 在胚鼠真皮细胞辅助下形成的毛发数量为(207±15.948)根,明显高于缺乏胚鼠真皮细胞辅助时形成的毛发数量(67±8.963)根,差异有统计学意义(n=3,t =7.653,P=0.002).结论 胚胎期真皮信号可使体外扩增后的新生鼠真皮细胞的毛发诱导能力得到更充分的发挥.
目的 探討胚胎期真皮信號對體外擴增後的新生鼠真皮細胞毛髮誘導能力的影響.方法 將胎齡14 d的胚鼠真皮細胞,加入到由體外擴增3d的新生鼠真皮細胞與新鮮分離的新生鼠錶皮細胞在裸鼠揹部構建的小室中,將其毛髮再生情況與未添加胚鼠真皮細胞時做比較.同時,分彆以下列細胞構建小室作為對照:胚鼠真皮細胞+新生鼠錶皮細胞；新鮮分離的新生鼠真皮細胞+新生鼠錶皮細胞；擴增的新生鼠真皮細胞；胚鼠真皮細胞；新鮮分離的新生鼠真皮細胞；新生鼠錶皮細胞.結果 在胚鼠真皮細胞輔助下形成的毛髮數量為(207±15.948)根,明顯高于缺乏胚鼠真皮細胞輔助時形成的毛髮數量(67±8.963)根,差異有統計學意義(n=3,t =7.653,P=0.002).結論 胚胎期真皮信號可使體外擴增後的新生鼠真皮細胞的毛髮誘導能力得到更充分的髮揮.
목적 탐토배태기진피신호대체외확증후적신생서진피세포모발유도능력적영향.방법 장태령14 d적배서진피세포,가입도유체외확증3d적신생서진피세포여신선분리적신생서표피세포재라서배부구건적소실중,장기모발재생정황여미첨가배서진피세포시주비교.동시,분별이하렬세포구건소실작위대조:배서진피세포+신생서표피세포；신선분리적신생서진피세포+신생서표피세포；확증적신생서진피세포；배서진피세포；신선분리적신생서진피세포；신생서표피세포.결과 재배서진피세포보조하형성적모발수량위(207±15.948)근,명현고우결핍배서진피세포보조시형성적모발수량(67±8.963)근,차이유통계학의의(n=3,t =7.653,P=0.002).결론 배태기진피신호가사체외확증후적신생서진피세포적모발유도능력득도경충분적발휘.
Objective To investigate the effect of embryonic dermal signal on the hair-inductive capacity of neonatal mice dermal cells which have been amplified in vitro.Methods Embryonic mice dermal cells of embryonic day 14 were added to a chamber on the back of nude mice with neonatal mice dermal cells which had been amplified in vitro for 3 days and frcshly isolated neonatal mice epidermal cells.The hair regeneration was compared between the groups with or without embryonic mice dermal cells.Meanwhile,chambers with following cells respectively were constructed as controls:embryonic mice dermal cells + neonatal mice epidermal cells; freshly isolated neonatal mice dermal cells + neonatal mice epidermal cells; amplified neonatal mice dermal cells only; embryonic mice dermal cells only; freshly isolated neonatal mice dermal cells only; neonatal mice epidermal cells only.Results The number of regenerated hairs with the aid of embryonic mice dermal cells(207 ± 15.948) was significantly higher than that(67 ± 8.963) in the group without embryonic mice dermal cells(n =3,t =7.653,P =0.002).Conclusion Embryonic dermal signal can enhance the hair-inductive capacity of neonatal mice dermal cells which have been amplified in vitro.
