中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2013年
3期
202-205
,共4页
卢强%陈英标%戴帆%仇树林
盧彊%陳英標%戴帆%仇樹林
로강%진영표%대범%구수림
山莨宕碱%外科皮瓣%再灌注损伤
山莨宕堿%外科皮瓣%再灌註損傷
산랑탕감%외과피판%재관주손상
Anisodamine%Surgical flaps%Reperfusion injury
目的 观察山莨宕碱注射液对大鼠缺血再灌注皮瓣成活面积及组织学变化的影响,以期证实山莨宕碱具有防治皮瓣坏死、提高皮瓣成活率的疗效.方法 48只大鼠随机分为缺血再灌注盐水组、缺血再灌注山莨宕碱处理组及对照组,建立以腹壁浅血管为蒂的轴型皮瓣,3组大鼠于掀起皮瓣后即刻及掀起皮瓣后12、18、34 h分别于皮瓣中段取材,检测皮瓣组织中超氧化物歧化酶(SOD)、一氧化氮(N0)及核因子NF-κB的含量,显微镜下观察皮瓣组织病理学变化情况,并于术后第7天观测皮瓣成活率.结果 缺血再灌注山莨宕碱处理组皮瓣成活率为(78.6％ ±7.3)％,再灌注后2、8、24 h皮瓣组织中SOD含量为(103.3±3.9)、(82.6±3.8)、(67.5±4.6) U/mg,NO含量为(5.33±2.05)、(4.75±1.68)、(4.15±1.59) nmol/mg,NF-κB含量为0.211±0.039、0.313±0.033、0.096±0.028.3组大鼠皮瓣组织中SOD、NO及NF-κB的含量在不同时刻相比较差异均有统计学意义；山莨宕碱处理组大鼠皮瓣病理变化情况明显好于盐水组；山莨宕碱处理组皮瓣成活率明显高于盐水组.结论 山莨宕碱能够减轻再灌注后皮瓣组织内自由基损伤,增加皮瓣术后血运,减少核因子NF-κB的产生,减轻炎性反应,增强皮瓣抗感染能力,从而可有效减轻皮瓣缺血再灌注损伤,降低皮瓣坏死率,提高皮瓣移植成活率.
目的 觀察山莨宕堿註射液對大鼠缺血再灌註皮瓣成活麵積及組織學變化的影響,以期證實山莨宕堿具有防治皮瓣壞死、提高皮瓣成活率的療效.方法 48隻大鼠隨機分為缺血再灌註鹽水組、缺血再灌註山莨宕堿處理組及對照組,建立以腹壁淺血管為蒂的軸型皮瓣,3組大鼠于掀起皮瓣後即刻及掀起皮瓣後12、18、34 h分彆于皮瓣中段取材,檢測皮瓣組織中超氧化物歧化酶(SOD)、一氧化氮(N0)及覈因子NF-κB的含量,顯微鏡下觀察皮瓣組織病理學變化情況,併于術後第7天觀測皮瓣成活率.結果 缺血再灌註山莨宕堿處理組皮瓣成活率為(78.6％ ±7.3)％,再灌註後2、8、24 h皮瓣組織中SOD含量為(103.3±3.9)、(82.6±3.8)、(67.5±4.6) U/mg,NO含量為(5.33±2.05)、(4.75±1.68)、(4.15±1.59) nmol/mg,NF-κB含量為0.211±0.039、0.313±0.033、0.096±0.028.3組大鼠皮瓣組織中SOD、NO及NF-κB的含量在不同時刻相比較差異均有統計學意義；山莨宕堿處理組大鼠皮瓣病理變化情況明顯好于鹽水組；山莨宕堿處理組皮瓣成活率明顯高于鹽水組.結論 山莨宕堿能夠減輕再灌註後皮瓣組織內自由基損傷,增加皮瓣術後血運,減少覈因子NF-κB的產生,減輕炎性反應,增彊皮瓣抗感染能力,從而可有效減輕皮瓣缺血再灌註損傷,降低皮瓣壞死率,提高皮瓣移植成活率.
목적 관찰산랑탕감주사액대대서결혈재관주피판성활면적급조직학변화적영향,이기증실산랑탕감구유방치피판배사、제고피판성활솔적료효.방법 48지대서수궤분위결혈재관주염수조、결혈재관주산랑탕감처리조급대조조,건립이복벽천혈관위체적축형피판,3조대서우흔기피판후즉각급흔기피판후12、18、34 h분별우피판중단취재,검측피판조직중초양화물기화매(SOD)、일양화담(N0)급핵인자NF-κB적함량,현미경하관찰피판조직병이학변화정황,병우술후제7천관측피판성활솔.결과 결혈재관주산랑탕감처리조피판성활솔위(78.6％ ±7.3)％,재관주후2、8、24 h피판조직중SOD함량위(103.3±3.9)、(82.6±3.8)、(67.5±4.6) U/mg,NO함량위(5.33±2.05)、(4.75±1.68)、(4.15±1.59) nmol/mg,NF-κB함량위0.211±0.039、0.313±0.033、0.096±0.028.3조대서피판조직중SOD、NO급NF-κB적함량재불동시각상비교차이균유통계학의의；산랑탕감처리조대서피판병리변화정황명현호우염수조；산랑탕감처리조피판성활솔명현고우염수조.결론 산랑탕감능구감경재관주후피판조직내자유기손상,증가피판술후혈운,감소핵인자NF-κB적산생,감경염성반응,증강피판항감염능력,종이가유효감경피판결혈재관주손상,강저피판배사솔,제고피판이식성활솔.
Objective To observe the effect of anisodamine (Ani) injection on the survival rate and histologic result of flaps with ischemia-reperfusion injury,so as to demonstrate the protective effect of Ani on the flap survival.Methods A total of 48 healthy male Wistar rats were randomly divided into model control,normal saline(NS) and anisodamine groups,with 16 rats in each group.An 3 cm × 6 cm axial flap was formed at the right lower abdomen with abdominal superficial blood vessel as the pedicle.0.5 cm ×0.5 cm skin tissue was taken from the middle part of flaps in each group,immediately after operation,12,18,24 h after operation.The superoxide dismutase (SOD),nitric oxide(NO),nuclear factor-κB contents in the specimens were detected.The histologic study was also performed.The flap survival rate was recorded 7 days after operation.Results Flap survival rate was (78.6 ± 7.3) ％ in Anisodamine group.12,18,24 h after reperfusion injury,the SOD was (103.3 ± 3.9),(82.6 ± 3.8),(67.5 ± 4.6) U/mg ;the NO was (5.33 ± 2.05),(4.75 ± 1.68),(4.15 ± 1.59) nmol/mg ; the NF-κB was0.211 ± 0.039,0.313 ± 0.033,0.096 ± 0.028.The contents of SOD、NO and NF-κB had the statistical difference of at different time.The skin pathological changes in Anisodamine group was obviously better than those in NS group.Flap survival rate in Anisodamine group was significantly higher than that in NS group.Conclusions In the flap with ischemia-reperfusion injury,Anisodamine can reduce the damage of free radical,increase the blood flow,reduce the production of NF-κB,decrease inflammatory reaction.So Anisodamine can increase the survival rate of flaps with ischemia reperfusion injury.
