CAJ | 학술논문

目的研究p53基因第72位密码子(codon 72)多态性与剖官产术后病理性瘢痕发生的关系.方法对广州医学院第三附属医院产科行剖宫产术的303例女性,于术后3～5d抽取外周静脉血2 ml,用荧光分子信标PCR法检测其血液标本的p53基因codon 72多态性.分别于术后第3、6、12个月对所有病例进行电话随访,其中有19例随访至18个月,观察其病理性瘢痕发生情况,以及其与p53基因codon 72多态性位点基因型的关系.用SPSS 13.0软件进行统计学分析.结果 303例产妇中发生病理性瘢痕增生30例,273例为正常愈合.2组产妇p53基因codon 72 3种基因型(C/C、C/G和G/G)频率总体上有显著差异；病理性瘢痕增生组产妇C/C基因型频率为46.7％(14/30),高于正常组的17.6％ (48/273),差异有统计学意义(P＜0.01)；在病理性瘢痕增生产妇中,C/C基因型频率显著高于C/G型(33.3％,10/30)和G/G型(20.0％,6/30),差异均有统计学意义(P＜0.01)；在病理性瘢痕增生产妇中,C等位基因频率为63.3％,也显著高于G等位基因频率(36.7％,P＜0.01),OR值为2.30.结论 p53基因codon 72 C等位基因与剖宫产术后病理性瘢痕的发生有一定的关联性.
목적 연구p53기인제72위밀마자(codon 72)다태성여부관산술후병이성반흔발생적관계.방법 대엄주의학원제삼부속의원산과행부궁산술적303례녀성,우술후3～5d추취외주정맥혈2 ml,용형광분자신표PCR법검측기혈액표본적p53기인codon 72다태성.분별우술후제3、6、12개월대소유병례진행전화수방,기중유19례수방지18개월,관찰기병이성반흔발생정황,이급기여p53기인codon 72다태성위점기인형적관계.용SPSS 13.0연건진행통계학분석.결과 303례산부중발생병이성반흔증생30례,273례위정상유합.2조산부p53기인codon 72 3충기인형(C/C、C/G화G/G)빈솔총체상유현저차이；병이성반흔증생조산부C/C기인형빈솔위46.7％(14/30),고우정상조적17.6％ (48/273),차이유통계학의의(P＜0.01)；재병이성반흔증생산부중,C/C기인형빈솔현저고우C/G형(33.3％,10/30)화G/G형(20.0％,6/30),차이균유통계학의의(P＜0.01)；재병이성반흔증생산부중,C등위기인빈솔위63.3％,야현저고우G등위기인빈솔(36.7％,P＜0.01),OR치위2.30.결론 p53기인codon 72 C등위기인여부궁산술후병이성반흔적발생유일정적관련성.
Objective To study the relationship between gene p53 codon 72 polymorphism and pathological scar formation occurrence after caesarean section.Methods The method of molecular beacon with real-time PCR was applied to detect gene polymorphism of p53 codon 72 in blood samples taken from 303 pregnant women (within a week after caesarea section).The clinical visits were taken 3 times for 12th to 18th months to ascertain clinical formation of pathological scar and its relationship to genotype of p53.The chi-square method was used to analyze the relationship of p53 gene polymorphism and abnormal scar formation occurrence by statistical software SPSS 13.0.Results Total of 303 pregnant women were assayed.30 patients were found with pathological scar by clinical visit in the total 303 pregnant women.The genotype frequencies of total three types (C/C,C/G and G/G) of p53 gene codon 72 in patients with pathological scar are significantly different from that of normal pregnant woman.The frequency of C/C genotype in patients are higher than that of normal pregnant women (P ＜ 0.01).The frequency of C/C genotype in these patients with pathological scar is higher (46.7％,14/30) than C/G (33.0％,10/30,P ＜0.01) or G/G (20％,6/30) genotype(P ＜0.01).The C allele frequency in the patients is 63.7％.It is also higher than G allele (36.7 ％,P ＜ 0.01).The OR value is 2.30.Therefore the C allele of p53 gene codon 72 is a risk factor for pathological scar.Conclusions There was a certain relationship between p53 gene codon 72 C allele and pathological scar formation after caesarean section.