中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2014年
2期
118-121
,共4页
颜彤彤%陈敏亮%马奎%梁黎明%刘畅%赖林英%付小兵
顏彤彤%陳敏亮%馬奎%樑黎明%劉暢%賴林英%付小兵
안동동%진민량%마규%량려명%류창%뢰림영%부소병
成纤维细胞%肉毒毒素类%肌动蛋白类%肌球蛋白类
成纖維細胞%肉毒毒素類%肌動蛋白類%肌毬蛋白類
성섬유세포%육독독소류%기동단백류%기구단백류
Fibroblasts%Botulinum toxins%Actins%Myosins
目的 探讨不同浓度A型肉毒毒素(botulinum toxin type A,BTXA)对瘢痕内成纤维细胞中α平滑肌肌动蛋白(α smooth muscle actin,α-SMA)和肌球蛋白-Ⅱ(myosin-Ⅱ)表达水平的影响.方法 从手术切除的挛缩性瘢痕组织中分离培养原代成纤维细胞,选取3～5代成纤维细胞随机分为正常组、低浓度BTXA组(1.0 U/106cells)和高浓度BTXA组(2.5 U/106cells),于药物作用后1、4、7d观察成纤维细胞的生长状况,并采用Wstern blot对成纤维细胞中α-SMA和myosin-Ⅱ的表达进行测定.结果 正常组成纤维细胞生长状态良好;低浓度BTXA组第4天时细胞增殖速度减慢;高浓度BTXA组第4天时细胞部分出现核固缩,第7天时细胞排列较疏松,视野呈现较多凋亡的细胞.α-SMA和myosin-Ⅱ表达在第4天时正常组显著高于BTXA作用组(P＜0.05),第7天时低浓度BTXA组与高浓度BTXA组间比较,差异有统计学意义(P＜0.05).结论 BTXA能够促进成纤维细胞凋亡,减少成纤维细胞中α-SMA和myosin-Ⅱ的表达,且在一定范围内,浓度越高抑制作用越明显.
目的 探討不同濃度A型肉毒毒素(botulinum toxin type A,BTXA)對瘢痕內成纖維細胞中α平滑肌肌動蛋白(α smooth muscle actin,α-SMA)和肌毬蛋白-Ⅱ(myosin-Ⅱ)錶達水平的影響.方法 從手術切除的攣縮性瘢痕組織中分離培養原代成纖維細胞,選取3～5代成纖維細胞隨機分為正常組、低濃度BTXA組(1.0 U/106cells)和高濃度BTXA組(2.5 U/106cells),于藥物作用後1、4、7d觀察成纖維細胞的生長狀況,併採用Wstern blot對成纖維細胞中α-SMA和myosin-Ⅱ的錶達進行測定.結果 正常組成纖維細胞生長狀態良好;低濃度BTXA組第4天時細胞增殖速度減慢;高濃度BTXA組第4天時細胞部分齣現覈固縮,第7天時細胞排列較疏鬆,視野呈現較多凋亡的細胞.α-SMA和myosin-Ⅱ錶達在第4天時正常組顯著高于BTXA作用組(P＜0.05),第7天時低濃度BTXA組與高濃度BTXA組間比較,差異有統計學意義(P＜0.05).結論 BTXA能夠促進成纖維細胞凋亡,減少成纖維細胞中α-SMA和myosin-Ⅱ的錶達,且在一定範圍內,濃度越高抑製作用越明顯.
목적 탐토불동농도A형육독독소(botulinum toxin type A,BTXA)대반흔내성섬유세포중α평활기기동단백(α smooth muscle actin,α-SMA)화기구단백-Ⅱ(myosin-Ⅱ)표체수평적영향.방법 종수술절제적련축성반흔조직중분리배양원대성섬유세포,선취3～5대성섬유세포수궤분위정상조、저농도BTXA조(1.0 U/106cells)화고농도BTXA조(2.5 U/106cells),우약물작용후1、4、7d관찰성섬유세포적생장상황,병채용Wstern blot대성섬유세포중α-SMA화myosin-Ⅱ적표체진행측정.결과 정상조성섬유세포생장상태량호;저농도BTXA조제4천시세포증식속도감만;고농도BTXA조제4천시세포부분출현핵고축,제7천시세포배렬교소송,시야정현교다조망적세포.α-SMA화myosin-Ⅱ표체재제4천시정상조현저고우BTXA작용조(P＜0.05),제7천시저농도BTXA조여고농도BTXA조간비교,차이유통계학의의(P＜0.05).결론 BTXA능구촉진성섬유세포조망,감소성섬유세포중α-SMA화myosin-Ⅱ적표체,차재일정범위내,농도월고억제작용월명현.
Objective To investigate the effects of botulinum toxin type A (BTXA) on the expression of α smooth muscle actin(α-SMA)and myosin-Ⅱ of fibroblasts in scars.Methods Fibroblasts were isolated from tissue specimens of scars contracture.Cells from passages 3-5 were randomly divided into 3 groups (control group,low BTXA group (1 U/106Cells),and high BTXA group (2.5 U/ 106 Cells)).Growth condition of fibroblasts was observed at 1,4,7 day after BTXA treated.Changes of α-SMA and myosin-Ⅱ in fibroblasts were detected by Western blot.Results Fibroblasts grew well in control group.The proliferation was decreased 4 days later in BTXA groups.Lots of apoptotic cells were seen in high BTXA group at 7th day.Proteins of α-SMA and myosin-Ⅱ in fibroblasts were statistically different between BTXA group and control groups at 4th day (P ＜ 0.05).The expression of α-SMA and myosin-Ⅱ in low BTXA group was higher than that in high BTXA group at 7th day(P ＜ 0.05).Conclusions BTXA could induce the apoptosis of fibroblasts and decrease the expression of α-SMA and myosin-Ⅱ in fibroblasts.The inhibitory effect was strengthened with BTXA concentration increase within a certain range.
