肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
9期
588-590,596
,共4页
路喜安%齐广强%杨周亮%秦桂萍%郝利芬
路喜安%齊廣彊%楊週亮%秦桂萍%郝利芬
로희안%제엄강%양주량%진계평%학리분
CDX2%PTEN%Ki-67%DNA倍体%结直肠肿瘤%免疫组织化学%流式细胞术
CDX2%PTEN%Ki-67%DNA倍體%結直腸腫瘤%免疫組織化學%流式細胞術
CDX2%PTEN%Ki-67%DNA배체%결직장종류%면역조직화학%류식세포술
CDX2%FTEN%Ki-67%DNA ploidy%Colorectal neoplasms%Immunohistochmistry%Flow cytometry
目的 探讨CDX2、PTEN、Ki-67在结直肠癌中的表达及其与DNA倍体的相互关系.方法 采用免疫组织化学SP法检测50例结直肠癌组织和10例癌旁的正常肠黏膜组织中CDX2、PTEN及Ki-67的表达,运用流式细胞术(FCM)对50例结直肠癌组织做DNA倍体分析.结果 结直肠癌和正常肠黏膜组织CDX2的阳性率分别为82%(41/50)和100%(10/10),差异有统计学意义(P＜0.05).PTEN在两组的表达阳性率分别为64%(32/50)和90%(9/10),差异有统计学意义(P＜0.05).二者与肿瘤的TNM分期、组织分级、淋巴结转移、原发灶浸润程度相关.CDX2和PTEN与Ki-67呈负相关(r=-0.254,r=-0.340).二倍体肿瘤中CDX2的表达明显高于异倍体组(93.75%:76.47%),而PTEN在两组表达中差异无统计学意义.结论 CDX2与PTEN呈正相关,二者与Ki-67呈负相关,在调节肠上皮细胞增生分化方面PTEN对CDX2有调控作用.CDX2的下降与肿瘤恶性程度、预后有关.
目的 探討CDX2、PTEN、Ki-67在結直腸癌中的錶達及其與DNA倍體的相互關繫.方法 採用免疫組織化學SP法檢測50例結直腸癌組織和10例癌徬的正常腸黏膜組織中CDX2、PTEN及Ki-67的錶達,運用流式細胞術(FCM)對50例結直腸癌組織做DNA倍體分析.結果 結直腸癌和正常腸黏膜組織CDX2的暘性率分彆為82%(41/50)和100%(10/10),差異有統計學意義(P＜0.05).PTEN在兩組的錶達暘性率分彆為64%(32/50)和90%(9/10),差異有統計學意義(P＜0.05).二者與腫瘤的TNM分期、組織分級、淋巴結轉移、原髮竈浸潤程度相關.CDX2和PTEN與Ki-67呈負相關(r=-0.254,r=-0.340).二倍體腫瘤中CDX2的錶達明顯高于異倍體組(93.75%:76.47%),而PTEN在兩組錶達中差異無統計學意義.結論 CDX2與PTEN呈正相關,二者與Ki-67呈負相關,在調節腸上皮細胞增生分化方麵PTEN對CDX2有調控作用.CDX2的下降與腫瘤噁性程度、預後有關.
목적 탐토CDX2、PTEN、Ki-67재결직장암중적표체급기여DNA배체적상호관계.방법 채용면역조직화학SP법검측50례결직장암조직화10례암방적정상장점막조직중CDX2、PTEN급Ki-67적표체,운용류식세포술(FCM)대50례결직장암조직주DNA배체분석.결과 결직장암화정상장점막조직CDX2적양성솔분별위82%(41/50)화100%(10/10),차이유통계학의의(P＜0.05).PTEN재량조적표체양성솔분별위64%(32/50)화90%(9/10),차이유통계학의의(P＜0.05).이자여종류적TNM분기、조직분급、림파결전이、원발조침윤정도상관.CDX2화PTEN여Ki-67정부상관(r=-0.254,r=-0.340).이배체종류중CDX2적표체명현고우이배체조(93.75%:76.47%),이PTEN재량조표체중차이무통계학의의.결론 CDX2여PTEN정정상관,이자여Ki-67정부상관,재조절장상피세포증생분화방면PTEN대CDX2유조공작용.CDX2적하강여종류악성정도、예후유관.
Objective To investigate the expression of CDX2,PTEN and Ki-67 in colorectal carcinoma,and the correlations among CDX2,PTEN,Ki-67 and DNA ploidy. Methods Fifty samples of human colorectal carcinoma tissue and ten samples of normal mucosa adjacent to carcinoma were examined for CDX2,PTEN as well as Ki-67 by immunohistochmistry,and the flow cytometry was used to detect the status of DNA ploidy jn colorectal carcinoma.Results The expression of CDX2 in colorectal carcinoma and normal mucosa tissue were 82%(41/50) and 100%(10/10) respectively.The expression of CDX2 had significant difference compared to that in normal mucosa tissue.PTEN in colorectal carcinoma and normal mucosa were 64%(32/50)and 90%(9/10) respectively,the expression had significant difference.Both CDX2 and PTEN were related with staging and grading of tumor,metastasis of lymph nodes and infiltrating depth of carcinoma.Expression of CDX2 and PTEN was negatively correlated with the expression of Ki-67(r=-0.254,P＜0.05;r=-0.340,P＜0.01) respectively.The expression of CDX2 was increased in diploid colorectal cancer,as compared with that in heteroploidy colorectal cancer(93.75%and 76.47%).But the expression of PTEN had no difference between diploidy and heteroploidy carcinomas.Conclusion The expression of the CDX2 and of the PTEN were are positive in part of the colorectal carcinoma.Both CDX2 and PTEN's expression negatively correlate with Ki-67.CDX2,intestinal-specific homeobox genes,regulate cell proliferation and differentiation in the continuously renewed intestinal epitheium.PTEN positively regulates CDX2 gene expression.The down regulation of CDX2 protein is concerned with tumor maligancy and bad prognosis.
