肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
9期
597-599
,共3页
胡大为%敖亚洲%杨景哲%王立平%马丽辉%王助新
鬍大為%敖亞洲%楊景哲%王立平%馬麗輝%王助新
호대위%오아주%양경철%왕립평%마려휘%왕조신
肠肿瘤%流式细胞术%免疫组化%抗原%CD44v6%p27
腸腫瘤%流式細胞術%免疫組化%抗原%CD44v6%p27
장종류%류식세포술%면역조화%항원%CD44v6%p27
Intestinal neoplasms%Flow cytometry%Immuno histochemistry%Antigen%CD44%p27
目的 探讨CD44v6和p27在大肠癌中的表达及其临床意义.方法 对50例手术切除的大肠癌新鲜标本及相应的正常大肠黏膜,应用流式细胞术(FCM)检测CD44v6的表达;同步制备上述病例大肠癌组织石蜡标本,采用免疫组织化学方法检测大肠癌组织中p27的表达,结合病理及临床资料进行统计学分析.结果 CD44v6和p27在大肠癌组织及正常黏膜组织中表达存在明显差异(P＜0.01),而且CD44v6 和p27的表达与大肠癌的分化程度、有无淋巴结转移、有无远端器官转移、Duke分期及预后相关(P值均＜0.05),CD44v6的表达与大肠癌的浸润深度无明显相关(P＞0.05),但p27的表达与大肠癌的浸润深度明显相关(P＜0.05).结论 CD44v6和p27的表达与大肠癌的临床病理学特征密切相关,两者在大肠癌组织中的表达呈一定的负相关,同时检测二者对判断大肠癌的恶性程度、复发转移潜能,评价患者的预后及术后选择合理的治疗方案具有一定的参考价值.
目的 探討CD44v6和p27在大腸癌中的錶達及其臨床意義.方法 對50例手術切除的大腸癌新鮮標本及相應的正常大腸黏膜,應用流式細胞術(FCM)檢測CD44v6的錶達;同步製備上述病例大腸癌組織石蠟標本,採用免疫組織化學方法檢測大腸癌組織中p27的錶達,結閤病理及臨床資料進行統計學分析.結果 CD44v6和p27在大腸癌組織及正常黏膜組織中錶達存在明顯差異(P＜0.01),而且CD44v6 和p27的錶達與大腸癌的分化程度、有無淋巴結轉移、有無遠耑器官轉移、Duke分期及預後相關(P值均＜0.05),CD44v6的錶達與大腸癌的浸潤深度無明顯相關(P＞0.05),但p27的錶達與大腸癌的浸潤深度明顯相關(P＜0.05).結論 CD44v6和p27的錶達與大腸癌的臨床病理學特徵密切相關,兩者在大腸癌組織中的錶達呈一定的負相關,同時檢測二者對判斷大腸癌的噁性程度、複髮轉移潛能,評價患者的預後及術後選擇閤理的治療方案具有一定的參攷價值.
목적 탐토CD44v6화p27재대장암중적표체급기림상의의.방법 대50례수술절제적대장암신선표본급상응적정상대장점막,응용류식세포술(FCM)검측CD44v6적표체;동보제비상술병례대장암조직석사표본,채용면역조직화학방법검측대장암조직중p27적표체,결합병리급림상자료진행통계학분석.결과 CD44v6화p27재대장암조직급정상점막조직중표체존재명현차이(P＜0.01),이차CD44v6 화p27적표체여대장암적분화정도、유무림파결전이、유무원단기관전이、Duke분기급예후상관(P치균＜0.05),CD44v6적표체여대장암적침윤심도무명현상관(P＞0.05),단p27적표체여대장암적침윤심도명현상관(P＜0.05).결론 CD44v6화p27적표체여대장암적림상병이학특정밀절상관,량자재대장암조직중적표체정일정적부상관,동시검측이자대판단대장암적악성정도、복발전이잠능,평개환자적예후급술후선택합리적치료방안구유일정적삼고개치.
Objective To observe the expression and reveal clinical significance of CD44v6 and p27 in colorectal carcinoma.Methods Collecting 50 cases of fresh specimen of colorectal carcinoma and normal mucous from excision operation.CD44v6 expression was detected by means of FCM in 50 cages of colorectal carcinoma and corresponding normal mucous.p27 protein was studied by SP immunohistochemical method.Analysis of statistics was performed by combining clinical and patholoical materials.Results CD44v6 and p27 expression in colorectal carcinoma was significantly higher than that in normal mucous (P＜0.01).As well as the expression of CD44v6 and p27 was obviously associated with differentiated grade,lymph node metastasis,distant metastasis and Dukes stage(P＜0.05).The expression of CD44v6 was not evidently correlated with the depth of tumor invasion(P＞0.05).but the expression of p27 Was correlated with the depth of tumor invasion(P＜0.05).Conclusion The expression of CD44v6 and p27 is markedly related with clinicopathological characters of colorectal carcinoma.CD44v6 expression and p27 expression axe obvious negative relationship in colorectal carcinoma.There is a certain value of reference for assessing the malignant grade and the potential of recurrence and metastasis,evaluating patient prognosis and selecting rational treatment after operation.
