肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2012年
9期
597-599
,共3页
侯玉森%苏文%王艳峰%田志华%刘富才
侯玉森%囌文%王豔峰%田誌華%劉富纔
후옥삼%소문%왕염봉%전지화%류부재
肺肿瘤%调节性T细胞%NK%NKG2D%免疫逃逸
肺腫瘤%調節性T細胞%NK%NKG2D%免疫逃逸
폐종류%조절성T세포%NK%NKG2D%면역도일
Lung neoplasms%Treg%NK%NKG2D%Immune escape
目的 分析肺癌患者外周血中CD+4 CDHi25 CDLo127调节性T细胞及自然杀伤细胞(NK细胞)活化受体NKG2D的表达水平之间的关系,探讨其在肿瘤免疫逃逸机制中的作用及临床意义.方法 选择70例肺癌患者,均经病理确诊.采用流式细胞术(FCM)检测患者外周血中CD+4 CDHi25 CDLo127调节性T细胞、NK细胞及NKG2D表达水平,并以50名健康人为对照.结果 肺癌患者外周血中CD+4 CDHi25 CDLo127调节性T细胞比例较健康对照组明显升高[(8.4±4.1)％与(6.7±1.7)％],差异有统计学意义(t=3.09,P＜0.05)；肺癌组NK细胞比例与健康对照组比较[(15.6±8.3)％与(17.2±4.2)％],差异无统计学意义(t=-1.33,P＞0.05)；肺癌组NKG2D较健康对照组明显降低[(83.3±4.9)％与(87.4±2.9)％],差异有统计学意义(t=3.16,P＜ 0.05).CD+4 CDHi25 CDLo127调节性T细胞与NKG2D呈负相关性(r=-0.302,P＜0.05).结论 在肺癌患者外周血中调节性T细胞可能通过下调NKG2D,参与肿瘤免疫逃逸机制,二者可作为评估肺癌患者免疫功能状态及预后的参考指标.
目的 分析肺癌患者外週血中CD+4 CDHi25 CDLo127調節性T細胞及自然殺傷細胞(NK細胞)活化受體NKG2D的錶達水平之間的關繫,探討其在腫瘤免疫逃逸機製中的作用及臨床意義.方法 選擇70例肺癌患者,均經病理確診.採用流式細胞術(FCM)檢測患者外週血中CD+4 CDHi25 CDLo127調節性T細胞、NK細胞及NKG2D錶達水平,併以50名健康人為對照.結果 肺癌患者外週血中CD+4 CDHi25 CDLo127調節性T細胞比例較健康對照組明顯升高[(8.4±4.1)％與(6.7±1.7)％],差異有統計學意義(t=3.09,P＜0.05)；肺癌組NK細胞比例與健康對照組比較[(15.6±8.3)％與(17.2±4.2)％],差異無統計學意義(t=-1.33,P＞0.05)；肺癌組NKG2D較健康對照組明顯降低[(83.3±4.9)％與(87.4±2.9)％],差異有統計學意義(t=3.16,P＜ 0.05).CD+4 CDHi25 CDLo127調節性T細胞與NKG2D呈負相關性(r=-0.302,P＜0.05).結論 在肺癌患者外週血中調節性T細胞可能通過下調NKG2D,參與腫瘤免疫逃逸機製,二者可作為評估肺癌患者免疫功能狀態及預後的參攷指標.
목적 분석폐암환자외주혈중CD+4 CDHi25 CDLo127조절성T세포급자연살상세포(NK세포)활화수체NKG2D적표체수평지간적관계,탐토기재종류면역도일궤제중적작용급림상의의.방법 선택70례폐암환자,균경병리학진.채용류식세포술(FCM)검측환자외주혈중CD+4 CDHi25 CDLo127조절성T세포、NK세포급NKG2D표체수평,병이50명건강인위대조.결과 폐암환자외주혈중CD+4 CDHi25 CDLo127조절성T세포비례교건강대조조명현승고[(8.4±4.1)％여(6.7±1.7)％],차이유통계학의의(t=3.09,P＜0.05)；폐암조NK세포비례여건강대조조비교[(15.6±8.3)％여(17.2±4.2)％],차이무통계학의의(t=-1.33,P＞0.05)；폐암조NKG2D교건강대조조명현강저[(83.3±4.9)％여(87.4±2.9)％],차이유통계학의의(t=3.16,P＜ 0.05).CD+4 CDHi25 CDLo127조절성T세포여NKG2D정부상관성(r=-0.302,P＜0.05).결론 재폐암환자외주혈중조절성T세포가능통과하조NKG2D,삼여종류면역도일궤제,이자가작위평고폐암환자면역공능상태급예후적삼고지표.
Objective To detect the levels of CD+4 CDHi25 CDLo127 regulatory T (Treg) cells and NKG2D-expressing NK cells in peripheral blood of lung cancer patients and investigate their relationship,and explore their roles in the immune escape of lung cancer and clinical significance.Methods The levels of CD+4 CDHi25 CDLo127 Treg cells,NK cells and NKG2D-expressing NK cells were measured in peripheral blood of 70 lung cancer patients and 50 healthy controls.Results Compared with control group,the levels of CD+4 CDHi25 CDLo127 Treg cells enormously increased [(8.4±4.1) ％,(6.7±1.7) ％] (t =3.09,P ＜ 0.05),while the value of NKG2D obviously decreased [(83.3±4.9) ％,(87.4±2.9) ％] (t =3.16,P ＜ 0.05); And the group of NK cells is not different [(15.6±8.3) ％,(17.2±4.2) ％] (t =-1.33,P ＞ 0.05); NKG2D-expressing NK cells had a perfect negative correlation with CD+4 CDLo25 CDLo127 Treg cells (r =-0.302,P ＜ 0.05).Conclusion Accumulation of Treg cells in serum may lead to the down-modulation of NKG2D-expressing NK which participates the tumor immune escape.They may be potential indicators evaluating immune functions and prognosis of lung cancers.
