肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2012年
9期
622-624
,共3页
廉建红%杨宣琴%王晓娟%孙瑞芳%王晶
廉建紅%楊宣琴%王曉娟%孫瑞芳%王晶
렴건홍%양선금%왕효연%손서방%왕정
食管肿瘤%肿瘤,鳞状细胞%免疫组织化学%bcl-2
食管腫瘤%腫瘤,鱗狀細胞%免疫組織化學%bcl-2
식관종류%종류,린상세포%면역조직화학%bcl-2
Esophageal neoplasms%Neoplasms,squamous cell%Immunohistochemistry%bcl-2
目的 研究凋亡相关基因bcl-2在食管鳞状细胞癌及癌旁组织中的表达及其意义.方法 应用EnVision二步法检测62例食管鳞状细胞癌及癌旁组织中bcl-2蛋白的表达.结果 bcl-2在单纯增生、高级别上皮内瘤变及鳞状细胞癌组织中表达率分别为80.3 ％(49/61)、45.9 ％(28/61)及67.7％(20/62),而在正常食管黏膜组织中仅少量表达[3.3％(1/30)],正常黏膜组与其余各组间表达率差异有统计学意义(x2=54.437,P＜0.01).bcl-2蛋白表达与食管鳞状细胞癌的组织分化程度、浸润深度无相关性(x2=0.219、x2=5.878,均P＞0.05),但bcl-2蛋白的表达与淋巴结转移有相关性(x2=4.120,P＜0.05).结论 bcl-2可能在食管癌早期阶段发挥作用,其可能成为判断食管鳞状细胞癌预后的指标之一.
目的 研究凋亡相關基因bcl-2在食管鱗狀細胞癌及癌徬組織中的錶達及其意義.方法 應用EnVision二步法檢測62例食管鱗狀細胞癌及癌徬組織中bcl-2蛋白的錶達.結果 bcl-2在單純增生、高級彆上皮內瘤變及鱗狀細胞癌組織中錶達率分彆為80.3 ％(49/61)、45.9 ％(28/61)及67.7％(20/62),而在正常食管黏膜組織中僅少量錶達[3.3％(1/30)],正常黏膜組與其餘各組間錶達率差異有統計學意義(x2=54.437,P＜0.01).bcl-2蛋白錶達與食管鱗狀細胞癌的組織分化程度、浸潤深度無相關性(x2=0.219、x2=5.878,均P＞0.05),但bcl-2蛋白的錶達與淋巴結轉移有相關性(x2=4.120,P＜0.05).結論 bcl-2可能在食管癌早期階段髮揮作用,其可能成為判斷食管鱗狀細胞癌預後的指標之一.
목적 연구조망상관기인bcl-2재식관린상세포암급암방조직중적표체급기의의.방법 응용EnVision이보법검측62례식관린상세포암급암방조직중bcl-2단백적표체.결과 bcl-2재단순증생、고급별상피내류변급린상세포암조직중표체솔분별위80.3 ％(49/61)、45.9 ％(28/61)급67.7％(20/62),이재정상식관점막조직중부소량표체[3.3％(1/30)],정상점막조여기여각조간표체솔차이유통계학의의(x2=54.437,P＜0.01).bcl-2단백표체여식관린상세포암적조직분화정도、침윤심도무상관성(x2=0.219、x2=5.878,균P＞0.05),단bcl-2단백적표체여림파결전이유상관성(x2=4.120,P＜0.05).결론 bcl-2가능재식관암조기계단발휘작용,기가능성위판단식관린상세포암예후적지표지일.
Objective To study the expression and significance of bcl-2 protein in esophageal squamous cell cancer and the surrounding tissues.Methods EnVision method was used to analyze the expression of bcl-2 protein in tissues of esophageal squamous cell cancer and the surrounding tissues from 62 patients.Results Expressions rates of bcl-2 protein were 80.3 ％ (49/61),45.9 ％ (28/61) and 67.7 ％ (20/62) in simple hyperplasia,high grade intraepithelial neoplasia and squamous cell carcinoma tissues,respectively,but 3.3 ％ (1/30) in normal mucosa tissue.There were significant differences between normal esophageal mucosa group and other groups (x2 =54.437,P ＜ 0.01).The expression of bcl-2 had no differentiation in tissue differentiation grade and degree of invasion of carcinoma (x2 =0.219,x2 =5.878,P ＞ 0.05).But it had significant relationship between the expression of bcl-2 and lymph node metastasis (x2 =4.120,P ＜ 0.05).Conclusion bcl-2 may predicting the occurrence of esophageal squamous cell cancer in early stage,and may be regarded as an useful index for prognosis.
