CAJ | 학술논문

目的探讨核因子κB(NF-κB)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)在大肠癌组织中的表达,及其与大肠癌发生、发展的关系.方法收集60例大肠癌组织及36例结肠腺瘤组织,以癌旁正常肠黏膜组织为对照,应用免疫组织化学SABC法检测NF-κB、TNF-α及IL-6的蛋白表达情况,并分析其与大肠癌临床病理特征的相关关系.结果在大肠癌组织、癌旁正常组织及结肠腺瘤组织中NF-κB的阳性表达率分别为76.7％(46/60)、46.7％(28/60)、83.3％(30/36),TNF-α的阳性率分别为70.0 ％(42/60)、36.7％(22/60)、66.7％(24/36),IL-6蛋白阳性率分别为80.0％(48/60)、43.3％(26/60)、61.1％(22/36),在不同组织中的表达差异均具有统计学意义(均P＜0.05)；大肠癌组织中NF-κB的表达与TNF-α及IL-6的表达密切相关；NF-κB、TNF-α的表达与脉管受侵、淋巴结转移及分期相关,IL-6的表达与淋巴结转移及分期相关.结论 NF-κB及下游的炎症因子的高表达与太肠癌的临床病理特征密切相关,NF-κB途径在大肠癌的发生、发展中起着重要作用.
목적 탐토핵인자κB(NF-κB)、종류배사인자α(TNF-α)、백세포개소6(IL-6)재대장암조직중적표체,급기여대장암발생、발전적관계.방법 수집60례대장암조직급36례결장선류조직,이암방정상장점막조직위대조,응용면역조직화학SABC법검측NF-κB、TNF-α급IL-6적단백표체정황,병분석기여대장암림상병리특정적상관관계.결과 재대장암조직、암방정상조직급결장선류조직중NF-κB적양성표체솔분별위76.7％(46/60)、46.7％(28/60)、83.3％(30/36),TNF-α적양성솔분별위70.0 ％(42/60)、36.7％(22/60)、66.7％(24/36),IL-6단백양성솔분별위80.0％(48/60)、43.3％(26/60)、61.1％(22/36),재불동조직중적표체차이균구유통계학의의(균P＜0.05)；대장암조직중NF-κB적표체여TNF-α급IL-6적표체밀절상관；NF-κB、TNF-α적표체여맥관수침、림파결전이급분기상관,IL-6적표체여림파결전이급분기상관.결론 NF-κB급하유적염증인자적고표체여태장암적림상병리특정밀절상관,NF-κB도경재대장암적발생、발전중기착중요작용.
Objective To investigate the expression of nuclear factor kappa B (NF-κB),tumor necrosis factor α (TNF-α) and interleukin6 (IL-6) in human colorectal carcinoma tissues,and to explore their clinical significances in the genesis and development of colorectal cancer.Methods Sixty cases of colorectal cancer tissues and 36 cases of colorectal adenoma tissues were collected,60 cases of paracancerous normal colorectal tissues were the controls.Immunohistochemistry SABC method was used to detect the expression of NF-κB,TNF-α and IL-6 in each group respectively.The correlation of NF-κB,TNF-α and IL-6 with clinical pathologic features of colorectal cancer was analyzed.Results In colorectal carcinoma,adjacent normal colorectal tissues and colorectal adenoma tissues the positive expression rates of NF-κB were 76.7 ％ (46/60),46.7 ％ (28/60),83.3 ％ (30/36),the positive rates of TNF-α were 70.0 ％ (42/60),36.7 ％ (22/60),66.7 ％(24/36),the positive rates of IL-6 were 80.0 ％ (48/60),43.3 ％ (26/60),61.1％ (22/36).The differences were significant in each group (all P ＜ 0.05).The expression of NF-κB was closely associated with the expression of TNF-α and IL-6 respectively.In addition,the expression of NF-κB and TNF-α were correlated with vascular invaded,lymphnode metastasis and different stages.The expression of IL-6 was correlated with lymphnode metastasis and different stages.Conclusion The over expression of NF-κB and the downriver inflammation factors have close relationship with biological behaviors of colorectal cancer.It may be considered that the pathway of NF-κB play an important role in the genesis and development of colorectal cancer.