CAJ | 학술논문

目的研究肝癌组织中CD133蛋白表达情况及其临床意义.方法应用免疫组织化学ABC法检测190例原发性肝细胞肝癌患者手术切除肿瘤组织中CD133蛋白的表达,分析其与临床病理特征和预后的关系.结果 190例患者中CD133阳性42例(22.1％).CD133蛋白表达与肝癌分化程度、镜下血管侵犯、乙型肝炎病毒感染有关(P＜ 0.001、P=0.016、P=0.024).单因素方差分析结果表明肝癌分化程度、淋巴结转移、肉眼血管侵犯、镜下血管侵犯、肝癌TNM分期以及CD133蛋白表达情况与肝癌切除术后患者无瘤生存有关(均P＜ 0.05).多因素方差分析结果表明CD133蛋白表达情况与镜下血管侵犯是肝癌切除术后患者无瘤生存的独立预测指标.生存分析表明CD133蛋白表达阳性组术后无瘤生存期明显短于CD133阴性组.结论肝癌组织中CD133蛋白的表达与肝癌的侵袭转移及预后密切相关.
목적 연구간암조직중CD133단백표체정황급기림상의의.방법 응용면역조직화학ABC법검측190례원발성간세포간암환자수술절제종류조직중CD133단백적표체,분석기여림상병리특정화예후적관계.결과 190례환자중CD133양성42례(22.1％).CD133단백표체여간암분화정도、경하혈관침범、을형간염병독감염유관(P＜ 0.001、P=0.016、P=0.024).단인소방차분석결과표명간암분화정도、림파결전이、육안혈관침범、경하혈관침범、간암TNM분기이급CD133단백표체정황여간암절제술후환자무류생존유관(균P＜ 0.05).다인소방차분석결과표명CD133단백표체정황여경하혈관침범시간암절제술후환자무류생존적독립예측지표.생존분석표명CD133단백표체양성조술후무류생존기명현단우CD133음성조.결론 간암조직중CD133단백적표체여간암적침습전이급예후밀절상관.
Objective To investigate the expression of CD133 protein in primary lesions of hepatocellular carcinoma (HCC) and its clinical significance.Methods The expression of CD133 protein in 190 patients with HCC was detected by immunohistochemical staining ABC method.The correlation of CD133 protein expression with the clinicopathologic parameters and features after operation was analyzed.Results The expression positive rate of CD133 protein in cancer tissues was 22.1％ (42/190).There was significant correlation between the expression of CD133 protein and tumor differentiation (P ＜ 0.001),micro vessel invasion (P =0.016) and hepatitis B virus infection (P =0.024).Univariate analysis of factors demonstrated that differentiation level,lymph node,macro vessel invasion,micro vessel invasion,TNM stage and CD133 expression were correlated with disease-free survival after surgery (all P ＜ 0.05).Multivariate analysis of factors demonstrated that both CD133 expression and micro vessel invasion were independent prognostic factors of disease-free survival after surgery.Prognostic analysis demonstrated that the disease-free survival of CD133 positive group was significantly lower than that of CD133 negative group.Conclusion The CD133 protein expression in HCC tissues is related with development,metastasis and prognosis of HCC.