CAJ | 학술논문

目的证实人类乳腺癌MCF-7细胞系中存在乙醛脱氢酶阳性(ALDH+)乳腺癌干细胞,并研究ALDH+乳腺癌干细胞体外增殖、侵袭能力等生物学特性.方法应用流式细胞术从MCF-7细胞中分离并培养ALDH+乳腺癌干/祖细胞,通过划痕试验、MTT法生长曲线测定以及Transwell法等检测ALDH+乳腺癌干细胞的生物学特性.结果 MCF-7细胞系中,CD-/low24CD+44细胞比例约为1.4％,ALDH+CD-/low24CD+44细胞比例约为1.2％;ALDH+乳腺癌干细胞与CD-/low24CD+44细胞的生长曲线基本一致；CD-/low24CD+44和ALDH+CD-/low24CD+44组细胞划痕区细胞间距离明显缩短,其迁移能力明显强于对照组,且两群干细胞之间存在差异；Transwell实验结果,与对照组相比,CD-/low24CD+44、ALDH+ CD-/low24CD+44两组细胞有大量细胞过膜,三组MTT检测吸光度值分别为1.05±0.098、1.56±0.075、1.67±0.032.结论 MCF-7细胞系中存在CD-/low24CD+44和ALDH+ CD-/low24CD+44乳腺癌干细胞,ALDH可作为鉴定乳腺癌干细胞的分子标志之一.
목적 증실인류유선암MCF-7세포계중존재을철탈경매양성(ALDH+)유선암간세포,병연구ALDH+유선암간세포체외증식、침습능력등생물학특성.방법 응용류식세포술종MCF-7세포중분리병배양ALDH+유선암간/조세포,통과화흔시험、MTT법생장곡선측정이급Transwell법등검측ALDH+유선암간세포적생물학특성.결과 MCF-7세포계중,CD-/low24CD+44세포비례약위1.4％,ALDH+CD-/low24CD+44세포비례약위1.2％;ALDH+유선암간세포여CD-/low24CD+44세포적생장곡선기본일치；CD-/low24CD+44화ALDH+CD-/low24CD+44조세포화흔구세포간거리명현축단,기천이능력명현강우대조조,차량군간세포지간존재차이；Transwell실험결과,여대조조상비,CD-/low24CD+44、ALDH+ CD-/low24CD+44량조세포유대량세포과막,삼조MTT검측흡광도치분별위1.05±0.098、1.56±0.075、1.67±0.032.결론 MCF-7세포계중존재CD-/low24CD+44화ALDH+ CD-/low24CD+44유선암간세포,ALDH가작위감정유선암간세포적분자표지지일.
Objective To identify a aldehyde dehydrogenases positive (ALDH+) cancer stem cell subpopulation in MCF-7 cells and to investigate the proliferation and differentiation characteristics of these cells in vitro.Methods ALDH+ breast cancer stem cells were isolated from MCF-7 cells by flow cytometry and the biological property of ALDH+ breast cancer stem cells were examined by scarification test,MTT,growth curvature and Transwell migration assay.Results The ratio of CD-/low24 CD+44 cells was about 1.4 ％ in MCF-7 cells.The ratio of ALDH+ CD-/low24CD+44 cells was about 1.2 ％.The growth curvature of ALDH+ breast cancer stem cells was almost the same with that of CD-/low24 CD+44 cells.The distance between cells was obviously shorter in both CD-/low24 CD+44 cells scarification zone and ALDH+ CD-/low24 CD+44 cells scarification zone.The migration ability of CD-/low24CD+44 cells and ALDH+ CD-/low24CD+44 cells was stronger than control group cells.There were migration ability differences between CD-/low24CD+44 cells and ALDH+ CD-/low24CD+44 cells.The results of Transwell experiments were in coincidence with above results.Lots of CD-/low24CD+44 cells and ALDH+ CD-/low24CD+44 cells were through the membrane.In MTT assay,absorbance values were 1.05±0.098,1.56±0.075 and 1.67±0.032.Conclusion CD--/low24CD+44 and ALDH+ CD-/low24CD+44 breast cancer stem cell subpopulation exist in MCF-7 cells.ALDH could potentially be used as a molecular marker to identify breast cancer stem cell subpopulation.