中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2013年
5期
50-52
,共3页
新生血管性青光眼%视黄醇结合蛋白4%中性粒细胞激活肽-78
新生血管性青光眼%視黃醇結閤蛋白4%中性粒細胞激活肽-78
신생혈관성청광안%시황순결합단백4%중성립세포격활태-78
Novascular glaucoma%Retinol binding protein 4%Epithelial neutrophil-activing peptide-78
目的 测定新生血管性青光眼(NVG)患者血清及房水中视黄醇结合蛋白4(RBP4)、中性粒细胞激活肽-78(ENA-78)的水平,探讨RBP4、ENA-78在NVG发生、发展中的作用.方法 选取NVG患者40例(40只眼)作为实验组(A组),原发性慢性闭角型青光眼患者(B组)、老年性白内障患者(C组)各40例(40只眼)作为对照组.采集三组患者血清及房水标本,通过双抗体夹心酶联免疫吸附试验(ELISA)法分别检测血清与房水中RBP4、ENA-78的水平.结果 ①A组房水中RBP4、ENA-78的水平(84±6) mg/L、(249±16) ng/L明显高于B组的(71±7) mg/L、(172±15) ng/L和C组的(59 ±6) mg/L、(85 ±12) ng/L,三组间两两比较,差异均有统计学意义(F=87.16,P＜0.01;F=75.39,P＜0.01).A组血清中RBP4、ENA-78的水平(57 ±6) mg/L、(184±16) ng/L高于B组的(47±5) mg/L、(135±15) ng/L和C组的(34 ±6) mg/L、(64±12) ng/L,三组间两两比较,差异均有统计学意义(F =73.46,P＜0.01F =81.23,P＜0.01).②A组房水和血清中RBP4与ENA-78的水平呈正相关(r=0.72,0.67,P均＜0.01),其余各组标本中无显著的相关性(P＞0.05).结论 NVG患者房水及血清中RBP4、ENA-78的水平明显高于对照组,且房水中二者水平呈明显正相关,提示在NVG病理机制过程中,RBP4、ENA-78作为促血管生成因子,相互促进、相互影响,共同导致了NVG的发生和发展.
目的 測定新生血管性青光眼(NVG)患者血清及房水中視黃醇結閤蛋白4(RBP4)、中性粒細胞激活肽-78(ENA-78)的水平,探討RBP4、ENA-78在NVG髮生、髮展中的作用.方法 選取NVG患者40例(40隻眼)作為實驗組(A組),原髮性慢性閉角型青光眼患者(B組)、老年性白內障患者(C組)各40例(40隻眼)作為對照組.採集三組患者血清及房水標本,通過雙抗體夾心酶聯免疫吸附試驗(ELISA)法分彆檢測血清與房水中RBP4、ENA-78的水平.結果 ①A組房水中RBP4、ENA-78的水平(84±6) mg/L、(249±16) ng/L明顯高于B組的(71±7) mg/L、(172±15) ng/L和C組的(59 ±6) mg/L、(85 ±12) ng/L,三組間兩兩比較,差異均有統計學意義(F=87.16,P＜0.01;F=75.39,P＜0.01).A組血清中RBP4、ENA-78的水平(57 ±6) mg/L、(184±16) ng/L高于B組的(47±5) mg/L、(135±15) ng/L和C組的(34 ±6) mg/L、(64±12) ng/L,三組間兩兩比較,差異均有統計學意義(F =73.46,P＜0.01F =81.23,P＜0.01).②A組房水和血清中RBP4與ENA-78的水平呈正相關(r=0.72,0.67,P均＜0.01),其餘各組標本中無顯著的相關性(P＞0.05).結論 NVG患者房水及血清中RBP4、ENA-78的水平明顯高于對照組,且房水中二者水平呈明顯正相關,提示在NVG病理機製過程中,RBP4、ENA-78作為促血管生成因子,相互促進、相互影響,共同導緻瞭NVG的髮生和髮展.
목적 측정신생혈관성청광안(NVG)환자혈청급방수중시황순결합단백4(RBP4)、중성립세포격활태-78(ENA-78)적수평,탐토RBP4、ENA-78재NVG발생、발전중적작용.방법 선취NVG환자40례(40지안)작위실험조(A조),원발성만성폐각형청광안환자(B조)、노년성백내장환자(C조)각40례(40지안)작위대조조.채집삼조환자혈청급방수표본,통과쌍항체협심매련면역흡부시험(ELISA)법분별검측혈청여방수중RBP4、ENA-78적수평.결과 ①A조방수중RBP4、ENA-78적수평(84±6) mg/L、(249±16) ng/L명현고우B조적(71±7) mg/L、(172±15) ng/L화C조적(59 ±6) mg/L、(85 ±12) ng/L,삼조간량량비교,차이균유통계학의의(F=87.16,P＜0.01;F=75.39,P＜0.01).A조혈청중RBP4、ENA-78적수평(57 ±6) mg/L、(184±16) ng/L고우B조적(47±5) mg/L、(135±15) ng/L화C조적(34 ±6) mg/L、(64±12) ng/L,삼조간량량비교,차이균유통계학의의(F =73.46,P＜0.01F =81.23,P＜0.01).②A조방수화혈청중RBP4여ENA-78적수평정정상관(r=0.72,0.67,P균＜0.01),기여각조표본중무현저적상관성(P＞0.05).결론 NVG환자방수급혈청중RBP4、ENA-78적수평명현고우대조조,차방수중이자수평정명현정상관,제시재NVG병리궤제과정중,RBP4、ENA-78작위촉혈관생성인자,상호촉진、상호영향,공동도치료NVG적발생화발전.
Objective To investigate the role of retinol binding protein 4 (RBP4) and epithelial neutrophil-activing peptide-78(ENA-78) in the development of neovascular glaucoma(NVG),by detecting the NVG serum and aqueous humor RBP4 and ENA-78 levels.Methods Forty patients with NVG were selected as experimental group(group A),40 patients with primary chronic angle closure glaucoma patients(group B),and 40 cases of senile cataract patients(group C)as a control group.Three specimens of serum and aqueous humor were collected and the serum and aqueous humor RBP4,ENA-78 levels were measured through the double-antibody sandwich enzyme-linked immuno sorbent assay(ELISA).Results ①The levels of RBP4 and ENA-78 in aqueous humor:group A[(84 ±6) mg/L,(249 ±16) ng/L] were significantly higher than that of group B[(71 ±7) mg/L,(172 ± 15) ng/L] and the group C[(59 ±6) mg/L,(85 ± 12) ng/L].Among the three groups,both the differences of pairwise comparison were significant(F =87.16,P ＜0.01 ; F =75.39,P ＜0.01).The levels of serum RBP4,ENA-78:group A[(57 ±6) mg/L,(184 ± 16) ng/L] were higher than that of the group B[(47 ±5) mg/L,(135 ± 15) ng/L] and the group C[(34 ±6) mg/L,(64 ± 12) ng/L].Pairwise comparison among the three groups,the differences were statistically significant(F =73.46,P ＜0.01; F =81.23,P ＜ 0.01).②NVG group of RBP4 and ENA-78 concentration in serum and aqueous showed a significant positive correlation and statistically significant difference(r =0.72,0.67,P ＜0.01).The remaining specimens in each group showed no significant correlation (P ＞ 0.05).Conclusions The RBP4,ENA78 levels in the aqueous and serum of patients with NVG were significantly higher than those for the other two groups,and the correlation between the two levels in the aqueous humor was significant positive,suggesting that the RBP4,ENA-78 as an angiogenic factor,promotted and influenced in mutual,led to the occurrence and development of the NVG in the process of NVG pathogenesis.
