CAJ | 학술논문

目的研究外周血循环MUC1 mRNA表达水平与乳腺癌、肺癌、其他癌等疾病的相关性以及对乳腺癌等疾病的诊断价值.方法分别采集健康对照组、良性肿瘤组、乳腺癌组、肺癌组以及其他癌组入院患者的血清样本,采用实时定量荧光逆转录聚合酶链反应(RT-PCR)方法检测外周血循环MUC1 mRNA含量.结果良性肿瘤组外周血循环MUC1 mRNA表达水平及阳性率(10％)与健康对照组比较差异无统计学意义(P＞0.05),乳腺癌组外周血循环MUC1 mRNA表达水平及阳性率(50％)与健康对照组比较差异有统计学意义(P＜0.01),乳腺癌组中以浸润性导管癌最高,各种类型乳腺癌比较差异无统计学意义(P＞0.05),肺癌组外周血循环MUC1 mRNA表达水平及阳性率(60％)与健康对照组比较差异有统计学意义(P＜0.01),其他癌组外周血循环MUC1 mRNA表达水平及阳性率(20％)与健康对照组比较差异无统计学意义(P＞0.05).结论 MUC1 mRNA可以作为乳腺癌、肺癌的诊断标志物,但对乳腺癌分型的诊断没有价值.
목적 연구외주혈순배MUC1 mRNA표체수평여유선암、폐암、기타암등질병적상관성이급대유선암등질병적진단개치.방법 분별채집건강대조조、량성종류조、유선암조、폐암조이급기타암조입원환자적혈청양본,채용실시정량형광역전록취합매련반응(RT-PCR)방법검측외주혈순배MUC1 mRNA함량.결과 량성종류조외주혈순배MUC1 mRNA표체수평급양성솔(10％)여건강대조조비교차이무통계학의의(P＞0.05),유선암조외주혈순배MUC1 mRNA표체수평급양성솔(50％)여건강대조조비교차이유통계학의의(P＜0.01),유선암조중이침윤성도관암최고,각충류형유선암비교차이무통계학의의(P＞0.05),폐암조외주혈순배MUC1 mRNA표체수평급양성솔(60％)여건강대조조비교차이유통계학의의(P＜0.01),기타암조외주혈순배MUC1 mRNA표체수평급양성솔(20％)여건강대조조비교차이무통계학의의(P＞0.05).결론 MUC1 mRNA가이작위유선암、폐암적진단표지물,단대유선암분형적진단몰유개치.
Objective To study the correlation of expression of MUC1 mRNA in peripheral blood with breast cancer and lung cancer,and its diagnostic value on breast cancer.Methods The serum in health control group,benign tumor,breast cancer,lung cancer and other cancer groups was collected,then the expression of MUC1 mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR).Results MUC1 mRNA expression and positive rate (10％) in benign tumor group had not statistically significant correlation with healthy control group (P ＞ 0.05).The expression and positive rate (50％) in breast cancer group had significant difference with healthy control group (P ＞ 0.01),MUC1 mRNA expression of infiltrating ductal carcinoma of breast was the highest in this group.Various types of breast cancer had no significant difference (P ＞ 0.05).The expression and positive rate (60％) in lung cancer group had significant difference with healthy control group(P ＞ 0.01),and in other cancer group had no significant difference(P ＞ 0.05).Conclusions MUC1 mRNA can be the marker to diagnose breast cancer and lung cancer,but it has no value on diagnosis of breast cancer classification.