中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2014年
23期
4-7
,共4页
任建林%田林%赵素琴%梁建芳%郑绘霞%肖虹
任建林%田林%趙素琴%樑建芳%鄭繪霞%肖虹
임건림%전림%조소금%량건방%정회하%초홍
大肠癌%转移淋巴结%化疗药敏性%5-氟尿嘧啶%胸苷酸合成酶%胸苷磷酸化酶%dUTP焦磷酸酶
大腸癌%轉移淋巴結%化療藥敏性%5-氟尿嘧啶%胸苷痠閤成酶%胸苷燐痠化酶%dUTP焦燐痠酶
대장암%전이림파결%화료약민성%5-불뇨밀정%흉감산합성매%흉감린산화매%dUTP초린산매
Colon neoplasms%Lymph node metastases%Chemo-sensitivity%5-fluorouracil%Thymidylate synthase%Thymidine phosphorylase%dUTP pyrophosphatase
目的 观察、探讨大肠癌、切缘正常组织及其相应转移淋巴结中氟尿嘧啶类药物代谢酶胸苷酸合成酶(TS)、胸苷磷酸化酶(TP)和dUTP焦磷酸酶(dUTPase)的表达情况;研究是否存在大肠癌原发灶与相应淋巴结转移灶中TS、TP和dUTPase表达水平的差异,以及与5-氟尿嘧啶(5-ru)化疗敏感性的关系;评估TS、TP和dUTPase作为5-Fu化疗敏感性判定指标的可行性.方法 应用免疫组化方法检测33例大肠癌、切缘正常组织及相应转移淋巴结中TS、TP和dUTPase的表达情况.结果 33例大肠癌淋巴结转移灶中TS和dUTPase的表达在表达水平、染色范围和染色深度三个方面均高于原发灶,差异有统计学意义(P＜0.05);大肠癌原发灶和淋巴结转移灶中TP的表达均为高表达,无相关性;大肠癌原发灶与淋巴结转移灶的耐药性不尽一致.结论 大肠癌原发灶和淋巴结转移灶之间的TS和dUTPase表达水平比较差异有统计学意义,应根据转移灶本身的TS和dUTPase的表达水平选择化疗方案.
目的 觀察、探討大腸癌、切緣正常組織及其相應轉移淋巴結中氟尿嘧啶類藥物代謝酶胸苷痠閤成酶(TS)、胸苷燐痠化酶(TP)和dUTP焦燐痠酶(dUTPase)的錶達情況;研究是否存在大腸癌原髮竈與相應淋巴結轉移竈中TS、TP和dUTPase錶達水平的差異,以及與5-氟尿嘧啶(5-ru)化療敏感性的關繫;評估TS、TP和dUTPase作為5-Fu化療敏感性判定指標的可行性.方法 應用免疫組化方法檢測33例大腸癌、切緣正常組織及相應轉移淋巴結中TS、TP和dUTPase的錶達情況.結果 33例大腸癌淋巴結轉移竈中TS和dUTPase的錶達在錶達水平、染色範圍和染色深度三箇方麵均高于原髮竈,差異有統計學意義(P＜0.05);大腸癌原髮竈和淋巴結轉移竈中TP的錶達均為高錶達,無相關性;大腸癌原髮竈與淋巴結轉移竈的耐藥性不儘一緻.結論 大腸癌原髮竈和淋巴結轉移竈之間的TS和dUTPase錶達水平比較差異有統計學意義,應根據轉移竈本身的TS和dUTPase的錶達水平選擇化療方案.
목적 관찰、탐토대장암、절연정상조직급기상응전이림파결중불뇨밀정류약물대사매흉감산합성매(TS)、흉감린산화매(TP)화dUTP초린산매(dUTPase)적표체정황;연구시부존재대장암원발조여상응림파결전이조중TS、TP화dUTPase표체수평적차이,이급여5-불뇨밀정(5-ru)화료민감성적관계;평고TS、TP화dUTPase작위5-Fu화료민감성판정지표적가행성.방법 응용면역조화방법검측33례대장암、절연정상조직급상응전이림파결중TS、TP화dUTPase적표체정황.결과 33례대장암림파결전이조중TS화dUTPase적표체재표체수평、염색범위화염색심도삼개방면균고우원발조,차이유통계학의의(P＜0.05);대장암원발조화림파결전이조중TP적표체균위고표체,무상관성;대장암원발조여림파결전이조적내약성불진일치.결론 대장암원발조화림파결전이조지간적TS화dUTPase표체수평비교차이유통계학의의,응근거전이조본신적TS화dUTPase적표체수평선택화료방안.
Objective To observe the expression of thymidylate synthase (TS),thymidine phosphorylase(TP) and dUTPase in colorectal carcinoma,normal tissues and corresponding lymph node metastasis,to study whether there is difference in expression levels of TS,TP and dUTPase in primary tumors of colorectal carcinoma and corresponding lymph node metastasis,and the relationship with the chemosensitivity of 5-Fu,and to assess feasibility of determine indicators of TS,TP and dUTPase as 5-Fu chemosensitivity.Methods The expression of TS,TP and dUTPase in 33 cases of colorectal cancer,normal tissues and corresponding lymph node metastases were assessed by immunohistochemical method.Results The expression levels,dyeing and dyeing depth range of TS,TP and dUTPase in 33 cases of lymph node metastasis of colorectal carcinomas was higher than that in primary tumors in colorectal carcinomas,the difference was significant (P ＜ 0.05).The expression of TP in primary tumor and lymph node metastasis in colorectal carcinomas were highly expressed,and had no correlation.The resistant in primary tumor and lymph node metastasis in colorectal cancer were not consistent.Conclusions The expression of TS and dUTPase in primary tumor and lymph node metastasis in colorectal carcinomas have significant differences,so the chemotherapy should be selected according to their metastatic expression levels of TS and dUTPase.
