CAJ | 학술논문

目的对搭载神舟八号飞船的屎肠球菌进行突变株筛选,并鉴定和分析差异表达的蛋白质.方法利用Biolog生化反应板从搭载神舟八号飞船飞行后屎肠球菌中筛选突变株,提取突变株蛋白质并进行SDS-PAGE电泳检测,用BCA法测定总蛋白浓度后,采用同重同位素相对与绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)方法分析空间环境诱变屎肠球菌突变株和地面对照株之间的差异表达蛋白,并进行统计、分析和注释.结果通过Biolog表型筛选获得空间环境诱导屎肠球菌突变株LCT-EF20,该菌株能利用肌酐和L-苹果酸,而不能利用对羟基苯乙酸.差异蛋白组学分析发现了124个蛋白质表达的改变,其中50个蛋白表达上调,74个蛋白表达下调.结论太空环境可改变屎肠球菌的生物学特性,并影响大量蛋白质的表达,差异表达蛋白主要分布在代谢相关过程.
목적대탑재신주팔호비선적시장구균진행돌변주사선,병감정화분석차이표체적단백질.방법이용Biolog생화반응판종탑재신주팔호비선비행후시장구균중사선돌변주,제취돌변주단백질병진행SDS-PAGE전영검측,용BCA법측정총단백농도후,채용동중동위소상대여절대정량(isobaric tags for relative and absolute quantitation,iTRAQ)방법분석공간배경유변시장구균돌변주화지면대조주지간적차이표체단백,병진행통계、분석화주석.결과통과Biolog표형사선획득공간배경유도시장구균돌변주LCT-EF20,해균주능이용기항화L-평과산,이불능이용대간기분을산.차이단백조학분석발현료124개단백질표체적개변,기중50개단백표체상조,74개단백표체하조.결론태공배경가개변시장구균적생물학특성,병영향대량단백질적표체,차이표체단백주요분포재대사상관과정.
Objective To identify the proteins and analyze their differential expressions in space Enterococcus faecium (E.faecium) mutant loaded in Shenzhou 8 spacecraft. Methods E.faecium mutant loaded in Shenzhou 8 spacecraft was isolated with Biolog biochemical reaction plate. Total protein was extracted from E.faecium mutant and quantified by SDS-PAGE. After the total protein was measured by BCA, the differential expressions of proteins in space E.faecium mutant and ground control strain were analyzed by isobaric tags for relative and absolute quantitation (iTRAQ). Results The space mutant stain LCT-EF20 was obtained by screening its phenotype, which can use inosine and L-malic acid but cannot utilize p-hydroxy phenylacetic acid. Differential proteomic analysis showed that the expression was up-regulated in 50 and down-regulated in 74 out of the 124 proteins with their expression changed. Conclusion Space environment can affect the biological features of E. faecium mutants and the expression of proteins. The differentially expressed proteins are mainly involved in the bacterial metabolic process.