CAJ | 학술논문

目的观察凋亡相关斑点样蛋白(apoptosis-associated speck-like protein containing a CARD,ASC)和caspase-1在小鼠耳部皮肤急性光损伤模型中的表达并探讨其在急性光损伤中可能的作用机制.方法将10只小鼠分为5组,每组2只,分别对耳部照射30、90、150、300、600 mJ/cm2剂量的中波紫外线(ultraviolet B,UVB),测最小红斑量(minimal erythema dose,MED).将20只BALB/c小鼠随机分为正常对照组和照射4倍MED剂量UVB实验组,比较两组耳部皮肤临床和病理改变,免疫组化方法检测耳部皮肤ASC和caspase-1的表达.结果与对照组相比,实验组小鼠耳部皮肤出现红斑、水肿、毛细血管扩张.组织病理改变见表皮细胞水肿、核固缩,真皮血管扩张、管周淋巴细胞浸润等.免疫组化染色显示与对照组相比,实验组耳部表皮ASC和caspase-1表达增强,两组差异有统计学意义(t=65.0、56.5,P<0.01).结论 ASC和caspase-1在UVB致小鼠急性光损伤中具有一定的促损伤作用.
목적관찰조망상관반점양단백(apoptosis-associated speck-like protein containing a CARD,ASC)화caspase-1재소서이부피부급성광손상모형중적표체병탐토기재급성광손상중가능적작용궤제.방법장10지소서분위5조,매조2지,분별대이부조사30、90、150、300、600 mJ/cm2제량적중파자외선(ultraviolet B,UVB),측최소홍반량(minimal erythema dose,MED).장20지BALB/c소서수궤분위정상대조조화조사4배MED제량UVB실험조,비교량조이부피부림상화병리개변,면역조화방법검측이부피부ASC화caspase-1적표체.결과여대조조상비,실험조소서이부피부출현홍반、수종、모세혈관확장.조직병리개변견표피세포수종、핵고축,진피혈관확장、관주림파세포침윤등.면역조화염색현시여대조조상비,실험조이부표피ASC화caspase-1표체증강,량조차이유통계학의의(t=65.0、56.5,P<0.01).결론 ASC화caspase-1재UVB치소서급성광손상중구유일정적촉손상작용.
Objective To observe the expressions of apoptosis-associated speck (ASC)-like protein and caspase-1 in BALB/C mice following ultraviolet B (UVB)-induced acute light injury of skin and its possible mechanism. Methods Ten BALB/C mice were divided into five groups (2 in each group). Their ears were exposed to UVB irradiation at the doses of 30, 90, 150, 300, 600 mJ/cm2, respectively. The minimal erythema dose (MED) was measured. Twenty BALB/C mice were randomly divided into blank control group and experimental group (irradiated by 4 MED of UVB). Clinical and pathological changes of ear skin were compared between the two groups. Expressions of ASC-like protein and caspase-1 in ear skin were detected with immunohistochemical staining. Results Erythema, edema, telangiectasia, karyopyknosis, and perivascular lymphocytes infiltration occurred in experimental group but not in blank control group. Immunohistochemical staining showed that the expression levels of ASC-like protein and caspase-1 were significantly higher in experimental group than in blank control group (t=65.0 and 56.5, P<0.01). Conclusion ASC-like protein and caspase-1 are expressed in BALB/C mice following UVB-induced acute light injury.