检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2013年
1期
21-22
,共2页
熊志刚%黄小天%余洋%曾瑾忱
熊誌剛%黃小天%餘洋%曾瑾忱
웅지강%황소천%여양%증근침
UF‐1000i全自动尿沉渣分析仪%比对试验%红细胞%白细胞%管型
UF‐1000i全自動尿沉渣分析儀%比對試驗%紅細胞%白細胞%管型
UF‐1000i전자동뇨침사분석의%비대시험%홍세포%백세포%관형
Sysmex UF‐1000i automated urinary sediment analyzers%verification testred blood cell%red blood cell%white blood cell%tube type
目的探讨实验室内不同全自动尿沉渣分析仪检测结果之间的差异是否在允许范围之内,以提高检验结果的准确性、一致性、可比性.方法以Sysmex UF‐1000i全自动尿沉渣分析仪(LJ‐25)作为标准仪器,在两台全自动尿沉渣分析仪均处于正常状态下,双份检测新鲜尿液,获取红细胞计数(RBC/μL)、白细胞计数(WBC/μL)、上皮细胞计数(EC/μL)、管型计数(CAST/μL )及细菌计数(BACT/μL),将检测结果进行通过率计算及比对试验,并参照Sysmex公司的仪器说明书提供的标准作为各参数的允许范围进行评估.结果两仪器检测结果显示, RBC、WBC、EC、CAST及BACT比对通过率88.09%、88.09%、92.86%、52.38%、35.71%;线性相关系数(r)为0.998、0.997、0.993、0.417、0.971,CAST、BACT结果比较差异无统计学意义(P>0.05),RBC、WBC、EC结果比较差异有统计学意义(P<0.05).结论临床实验室应加强对所有检测项目结果的可靠性进行验证,做好尿沉渣分析仪室内质控的同时定期进行仪器比对试验,以保证不同仪器上的检测结果具有可比性、准确性、一致性、持续性的质量保证.
目的探討實驗室內不同全自動尿沉渣分析儀檢測結果之間的差異是否在允許範圍之內,以提高檢驗結果的準確性、一緻性、可比性.方法以Sysmex UF‐1000i全自動尿沉渣分析儀(LJ‐25)作為標準儀器,在兩檯全自動尿沉渣分析儀均處于正常狀態下,雙份檢測新鮮尿液,穫取紅細胞計數(RBC/μL)、白細胞計數(WBC/μL)、上皮細胞計數(EC/μL)、管型計數(CAST/μL )及細菌計數(BACT/μL),將檢測結果進行通過率計算及比對試驗,併參照Sysmex公司的儀器說明書提供的標準作為各參數的允許範圍進行評估.結果兩儀器檢測結果顯示, RBC、WBC、EC、CAST及BACT比對通過率88.09%、88.09%、92.86%、52.38%、35.71%;線性相關繫數(r)為0.998、0.997、0.993、0.417、0.971,CAST、BACT結果比較差異無統計學意義(P>0.05),RBC、WBC、EC結果比較差異有統計學意義(P<0.05).結論臨床實驗室應加彊對所有檢測項目結果的可靠性進行驗證,做好尿沉渣分析儀室內質控的同時定期進行儀器比對試驗,以保證不同儀器上的檢測結果具有可比性、準確性、一緻性、持續性的質量保證.
목적탐토실험실내불동전자동뇨침사분석의검측결과지간적차이시부재윤허범위지내,이제고검험결과적준학성、일치성、가비성.방법이Sysmex UF‐1000i전자동뇨침사분석의(LJ‐25)작위표준의기,재량태전자동뇨침사분석의균처우정상상태하,쌍빈검측신선뇨액,획취홍세포계수(RBC/μL)、백세포계수(WBC/μL)、상피세포계수(EC/μL)、관형계수(CAST/μL )급세균계수(BACT/μL),장검측결과진행통과솔계산급비대시험,병삼조Sysmex공사적의기설명서제공적표준작위각삼수적윤허범위진행평고.결과량의기검측결과현시, RBC、WBC、EC、CAST급BACT비대통과솔88.09%、88.09%、92.86%、52.38%、35.71%;선성상관계수(r)위0.998、0.997、0.993、0.417、0.971,CAST、BACT결과비교차이무통계학의의(P>0.05),RBC、WBC、EC결과비교차이유통계학의의(P<0.05).결론림상실험실응가강대소유검측항목결과적가고성진행험증,주호뇨침사분석의실내질공적동시정기진행의기비대시험,이보증불동의기상적검측결과구유가비성、준학성、일치성、지속성적질량보증.
Objective To compare the test results from the different Sysmex UF‐1000i automated urinary sed‐iment analyzers ,and to explore whether the variance of the results were within the permitted range for improving the accuracy and consistency .Methods Under the condition that two urinary sediment analyzers were normal ,two fresh urine samples were examined in duplicate ,and the test data of RBC/μL、WBC/μL、EC/μL、CAST/μL and BACT/μL were compared with the results determined by traceable urinary sediment analyzer ,and regression equations were es‐tablished ,then the passing rate was counted and test results were evaluated with the reference provided by SYSMEX company .Results The verification analyzers and the reference analyzer showed that RBC ,WBC ,EC ,CAST and BACT passing rates were 88 .09% ,88 .09% ,92 .86% ,52 .38% ,35 .71% ,coefficients of correlation (r) exceeded 0 .998 ,0 .997 ,0 .993 ,0 .417 ,0 .971 ,and there was no statistical significance of CAST and BACT between two analyz‐ers (P>0 .05) ,but statistical differences of RBC ,WBC and EC were observed(P<0 .05) .Conclusion Clinical labo‐ratory should strengthen the reliability of all project detection results for verification ,regular comparison test and complete the urinary sediment analyzers interior quality control for ensuring test results of different instruments com‐parable ,accuracy and consistency .
