检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2013年
1期
29-30
,共2页
胡鹤娟%徐银根%徐喜林%张雅娟
鬍鶴娟%徐銀根%徐喜林%張雅娟
호학연%서은근%서희림%장아연
生化检验%酶%葡萄糖%标本处理%质量控制
生化檢驗%酶%葡萄糖%標本處理%質量控製
생화검험%매%포도당%표본처리%질량공제
biochemical test%enzyme%glucose%Specimen handling%quality control
目的探讨标本不同处理因素对部分生化检验项目的影响.方法血液标本立即检测设为对照组,(1)标本放置3、7、24 h后检测丙氨酸氨基转移酶(ALT )、天门冬氨酸氨基转移酶(AST )、碱性磷酸酶(ALP)、γ‐谷氨酰转肽酶(GGT )、乳酸脱氢酶(LDH);(2)全血保存和血清分离保存4℃24 h ,检测ALT、LDH、血糖(GLU );(3)密封保存和未密封保存检测 ALP.结果(1)对照组、标本放置3、7、24 h各组 ALT 检测结果分别是(16.00 ± 6.48)、(16.01 ± 6.72)、(16.37 ± 6.92)、(16.57±7.09)U/L ,均 P>0.05;AST (18.3±4.72)、(18.2±4.68)、(19.1±4.33)、(17.8±4.75)U/L ,均 P>0.05;ALP (78.1±16.28)、(77.1±15.82)、(78.2±16.55)、(77.4±16.15)U/L ,均 P>0.05;GGT (11.4 ± 5.62)、(11.3 ± 5.65)、(12.0 ± 5.33)、(12.0 ± 5.69)U/L ,均 P>0.05;LDH (183.8±21.96)、(183.3±22.74)(P>0.05)、(188.4±25.15)(P<0.01)、(196.2±26.41)U/L (P<0.01);(2)对照组、全血保存、血清分离保存各组:ALT (11.29 ± 3.19)、(12.14 ± 3.07)、(11.41±2.97)U/L ,均 P>0.05;LDH (194.83±27.09)、(217.41±28.35)(P<0.01)、(202.75±27.22)U/L (P>0.05);GLU (4.88 ± 0.68)、(3.30 ± 0.81)(P<0.01)、(5.08 ± 0.80)mmol/L (P>0.05);(3)密封保存:对照组、全血保存、血清保存 ALP (74.33±11.79)、(74.60±12.05)、(74.66±11.61)U/L (均 P>0.05);未密封保存:对照组、全血保存、血清保存 ALP (70.80 ± 7.85)、(85.86 ± 6.65)(P<0.01)、(92.60 ± 5.67)U/L (P<0.01).结论标本放置3、7、24 h对 ALT、AST、ALP、GGT检测无明显影响,放置7、24 h对LDH检测有显著影响;全血保存24 h对LDH、GLU结果存在明显差异;未密封保存对生化指标有明显影响.应注意分析前质量控制,降低分析前误差.
目的探討標本不同處理因素對部分生化檢驗項目的影響.方法血液標本立即檢測設為對照組,(1)標本放置3、7、24 h後檢測丙氨痠氨基轉移酶(ALT )、天門鼕氨痠氨基轉移酶(AST )、堿性燐痠酶(ALP)、γ‐穀氨酰轉肽酶(GGT )、乳痠脫氫酶(LDH);(2)全血保存和血清分離保存4℃24 h ,檢測ALT、LDH、血糖(GLU );(3)密封保存和未密封保存檢測 ALP.結果(1)對照組、標本放置3、7、24 h各組 ALT 檢測結果分彆是(16.00 ± 6.48)、(16.01 ± 6.72)、(16.37 ± 6.92)、(16.57±7.09)U/L ,均 P>0.05;AST (18.3±4.72)、(18.2±4.68)、(19.1±4.33)、(17.8±4.75)U/L ,均 P>0.05;ALP (78.1±16.28)、(77.1±15.82)、(78.2±16.55)、(77.4±16.15)U/L ,均 P>0.05;GGT (11.4 ± 5.62)、(11.3 ± 5.65)、(12.0 ± 5.33)、(12.0 ± 5.69)U/L ,均 P>0.05;LDH (183.8±21.96)、(183.3±22.74)(P>0.05)、(188.4±25.15)(P<0.01)、(196.2±26.41)U/L (P<0.01);(2)對照組、全血保存、血清分離保存各組:ALT (11.29 ± 3.19)、(12.14 ± 3.07)、(11.41±2.97)U/L ,均 P>0.05;LDH (194.83±27.09)、(217.41±28.35)(P<0.01)、(202.75±27.22)U/L (P>0.05);GLU (4.88 ± 0.68)、(3.30 ± 0.81)(P<0.01)、(5.08 ± 0.80)mmol/L (P>0.05);(3)密封保存:對照組、全血保存、血清保存 ALP (74.33±11.79)、(74.60±12.05)、(74.66±11.61)U/L (均 P>0.05);未密封保存:對照組、全血保存、血清保存 ALP (70.80 ± 7.85)、(85.86 ± 6.65)(P<0.01)、(92.60 ± 5.67)U/L (P<0.01).結論標本放置3、7、24 h對 ALT、AST、ALP、GGT檢測無明顯影響,放置7、24 h對LDH檢測有顯著影響;全血保存24 h對LDH、GLU結果存在明顯差異;未密封保存對生化指標有明顯影響.應註意分析前質量控製,降低分析前誤差.
목적탐토표본불동처리인소대부분생화검험항목적영향.방법혈액표본립즉검측설위대조조,(1)표본방치3、7、24 h후검측병안산안기전이매(ALT )、천문동안산안기전이매(AST )、감성린산매(ALP)、γ‐곡안선전태매(GGT )、유산탈경매(LDH);(2)전혈보존화혈청분리보존4℃24 h ,검측ALT、LDH、혈당(GLU );(3)밀봉보존화미밀봉보존검측 ALP.결과(1)대조조、표본방치3、7、24 h각조 ALT 검측결과분별시(16.00 ± 6.48)、(16.01 ± 6.72)、(16.37 ± 6.92)、(16.57±7.09)U/L ,균 P>0.05;AST (18.3±4.72)、(18.2±4.68)、(19.1±4.33)、(17.8±4.75)U/L ,균 P>0.05;ALP (78.1±16.28)、(77.1±15.82)、(78.2±16.55)、(77.4±16.15)U/L ,균 P>0.05;GGT (11.4 ± 5.62)、(11.3 ± 5.65)、(12.0 ± 5.33)、(12.0 ± 5.69)U/L ,균 P>0.05;LDH (183.8±21.96)、(183.3±22.74)(P>0.05)、(188.4±25.15)(P<0.01)、(196.2±26.41)U/L (P<0.01);(2)대조조、전혈보존、혈청분리보존각조:ALT (11.29 ± 3.19)、(12.14 ± 3.07)、(11.41±2.97)U/L ,균 P>0.05;LDH (194.83±27.09)、(217.41±28.35)(P<0.01)、(202.75±27.22)U/L (P>0.05);GLU (4.88 ± 0.68)、(3.30 ± 0.81)(P<0.01)、(5.08 ± 0.80)mmol/L (P>0.05);(3)밀봉보존:대조조、전혈보존、혈청보존 ALP (74.33±11.79)、(74.60±12.05)、(74.66±11.61)U/L (균 P>0.05);미밀봉보존:대조조、전혈보존、혈청보존 ALP (70.80 ± 7.85)、(85.86 ± 6.65)(P<0.01)、(92.60 ± 5.67)U/L (P<0.01).결론표본방치3、7、24 h대 ALT、AST、ALP、GGT검측무명현영향,방치7、24 h대LDH검측유현저영향;전혈보존24 h대LDH、GLU결과존재명현차이;미밀봉보존대생화지표유명현영향.응주의분석전질량공제,강저분석전오차.
Objective To observe the impacts of different treatments of specimen on biochemical tests of some items .Methods The results that specimens were tested right away after collections were put into the matched group .(1) the indices of ALT ,AST ,ALP ,GGT and LDH were determined separately 3 hours ,7 hours and 24 hours after the blood were collected .(2) The indices of ALT ,LDH and GLU were determined separately when the blood specimens and the serum specimens were stored at 4 ℃ for 24 hours .(3) The index of ALP was determined when the blood specimens and the serum specimens were stored in the condition of sealed and unsealed at 4 ℃ for 24 hours . Results ALT of control sample and samples laid side for 3 ,7 and 24 hours were 16 .00 ± 6 .48 ,16 .01 ± 6 .72 ,16 .37 ± 6 .92 ,16 .57 ± 7 .09(P>0 .05) .AST of control sample and samples layed side for 3 ,7 and 24 hours were 18 .3 ± 4 .72 ,18 .2 ± 4 .68 ,19 .1 ± 4 .33 ,17 .8 ± 4 .75(P>0 .05) .ALP of samples layed side for 3 ,7 and 24 hours were 78 .1 ± 16 .28 ,77 .1 ± 15 .82 ,78 .2 ± 16 .55 ,77 .4 ± 16 .15(P>0 .05) .GGT of control sample and samples layed side for 3 , 7 and 24 hours were 11 .4 ± 5 .62 ,11 .3 ± 5 .65 ,12 .0 ± 5 .33 ,12 .0 ± 5 .69 (P>0 .05) .LDH of control sample and samples layed side for 3 ,7 and 24 hours were 183 .8 ± 21 .96 ,183 .3 ± 22 .74(P>0 .05) ,188 .4 ± 25 .15(P<0 .01) , 196 .2 ± 26 .41(P<0 .01) .ALT of control sample ,blood specimens and the separated serum specimens were 11 .29 ± 3 .19 ,12 .14 ± 3 .07 ,11 .41 ± 2 .97(P>0 .05) .LDH of control sample ,blood specimens and the separated serum specimens were 194 .83 ± 27 .09 ,217 .41 ± 28 .35(P<0 .01) ,202 .75 ± 27 .22(P>0 .05) .GLU of control sample , blood specimens and the separated serum specimens were 4 .88 ± 0 .68 ,3 .30 ± 0 .81(P< 0 .01) ,5 .08 ± 0 .80( P>0 .05) .For Sealed storage ,ALT of control sample ,blood specimens and the separated serum specimens were 74 .33 ± 11 .79 ,74 .60 ± 12 .05 ,74 .66 ± 11 .61(P>0 .05) .For unsealed storage ,ALT of control sample ,blood specimens and the separated serum specimens were 70 .80 ± 7 .85 ,85 .86 ± 6 .65(P<0 .01) ,92 .60 ± 5 .67 .Conclusion the time of laying aside ,the blood being stored and the un‐covered condition have the impacts on the results .
