基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
GENOMICS AND APPLIED BIOLOGY
2012年
6期
538-543
,共6页
水稻(Oryza sativa L.)%DET1%矮化%无限分蘖
水稻(Oryza sativa L.)%DET1%矮化%無限分蘗
수도(Oryza sativa L.)%DET1%왜화%무한분얼
Rice (Oryza sativa L.)%DET1%Dwarfing%Excessively-tillering
从 EMS 诱变日本晴种子的 M2代中筛选得到一个植株明显矮化、分蘖数急剧增多的突变体,命名为 det1(dwarf and excessively-tillering 1).det1还显示出明显的穗型、粒型和育性等方面的生殖发育缺陷.遗传分析表明,det1受一对隐性基因控制.以 det1与品种 DZ60杂交建立了 F2定位群体,利用微卫星标记分析F2群体中的突变体,将 DET1基因定位在第6染色体长臂端,标记 SSR2和 SSR3之间约68 kb 的范围内.已知该区间内存在一个与水稻株高和分蘖有关的基因 D3.测序结果表明,DET1与 D3是同一个基因,编码一个 LRR 型的 F-box 蛋白.与 d3相比,det1还显示了新的表型特征,推测可能是等位基因的不同突变位点造成的.det1/d3的表型特征提示,DET1/D3在调节水稻生长发育过程中具有多效性.因此,利用 det1开展进一步的研究将有助于全面揭示 DET1/D3的分子功能.
從 EMS 誘變日本晴種子的 M2代中篩選得到一箇植株明顯矮化、分蘗數急劇增多的突變體,命名為 det1(dwarf and excessively-tillering 1).det1還顯示齣明顯的穗型、粒型和育性等方麵的生殖髮育缺陷.遺傳分析錶明,det1受一對隱性基因控製.以 det1與品種 DZ60雜交建立瞭 F2定位群體,利用微衛星標記分析F2群體中的突變體,將 DET1基因定位在第6染色體長臂耑,標記 SSR2和 SSR3之間約68 kb 的範圍內.已知該區間內存在一箇與水稻株高和分蘗有關的基因 D3.測序結果錶明,DET1與 D3是同一箇基因,編碼一箇 LRR 型的 F-box 蛋白.與 d3相比,det1還顯示瞭新的錶型特徵,推測可能是等位基因的不同突變位點造成的.det1/d3的錶型特徵提示,DET1/D3在調節水稻生長髮育過程中具有多效性.因此,利用 det1開展進一步的研究將有助于全麵揭示 DET1/D3的分子功能.
종 EMS 유변일본청충자적 M2대중사선득도일개식주명현왜화、분얼수급극증다적돌변체,명명위 det1(dwarf and excessively-tillering 1).det1환현시출명현적수형、립형화육성등방면적생식발육결함.유전분석표명,det1수일대은성기인공제.이 det1여품충 DZ60잡교건립료 F2정위군체,이용미위성표기분석F2군체중적돌변체,장 DET1기인정위재제6염색체장비단,표기 SSR2화 SSR3지간약68 kb 적범위내.이지해구간내존재일개여수도주고화분얼유관적기인 D3.측서결과표명,DET1여 D3시동일개기인,편마일개 LRR 형적 F-box 단백.여 d3상비,det1환현시료신적표형특정,추측가능시등위기인적불동돌변위점조성적.det1/d3적표형특정제시,DET1/D3재조절수도생장발육과정중구유다효성.인차,이용 det1개전진일보적연구장유조우전면게시 DET1/D3적분자공능.
A mutant called det1 (dwarf and excessively-tillering 1) was identified in the M2 generation of Nipponbare seeds treated with EMS, which exhibited serious dwarf and an excessive increase in tiller number. The det1 mutant also showed obviously defects in reproductive development including panicle size, grain size and spikelet fertility, etc. Genetic analysis indicated that det1 should be controlled by a single recessive gene. In order to map the gene, an F2 population was constructed by crossing the det1 mutant with variety DZ60 (Oryza sativa L. indica). By analyz-ing mutant plants in the F2 population using microsatellite markers, DET1 was mapped to a 68 kb region between markers SSR2 and SSR3 on the long arm of chromosome 6 in rice. It has been known that a gene D3 controlling rice height and tillering existed in this region. Sequencing results suggested that DET1 and D3 should be the same gene, which encodes a leucine-rich repeat (LRR) typed F-box protein. Compared with d3, det1 exhibited some ad-ditional mutant phenotypes probably caused by their different mutation sites. The phenotypes of det1/d3 suggested that DET1/D3 should play pleiotropic roles in regulating rice growth and development. Therefore, comprehensive studies on det1 would facilitate complete understanding of the molecular function of DET1/D3.
