基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
GENOMICS AND APPLIED BIOLOGY
2012年
6期
587-591
,共5页
水稻%拟南芥%稻瘟菌%完整 SSR%不完整 SSR%多态性
水稻%擬南芥%稻瘟菌%完整 SSR%不完整 SSR%多態性
수도%의남개%도온균%완정 SSR%불완정 SSR%다태성
Rice%Arabidopsis%M. grisea%Perfect SSR%Imperfect SSR%Polymorphism
微卫星或简单序列重复(SSR)是指以1~6个碱基为单位的串联重复序列.SSR 包括完整型和不完整型两种.不完整型 SSR 是指其序列中存在个别不符合基序的碱基.SSR 作为一种用途广泛的分子标记,其多态性水平越高则应用价值越大.本研究利用模式植物水稻的两个品种(籼稻93-11和粳稻日本晴)和拟南芥的两个生态型(Columbia 和 Lansberg)的基因组序列,以及稻瘟菌中245个 SSR 标记的试验结果,对 SSR 序列完整性与多态性之间的关系进行了分析.结果表明,总体上三个物种都表现为不完整型 SSR 的多态性比完整型 SSR 低,其中以稻瘟菌最为显著.水稻和拟南芥表现出相似的规律,其 SSR 序列完整性对多态性的影响都是以二核苷酸重复最为显著,而五核苷酸重复序列则基本不受影响;另外,SSR 多态性水平与序列完整程度呈正相关关系,但轻微的序列不完整一般并不会降低 SSR 的多态性,甚至在拟南芥中还提高多态性.本研究结果对 SSR 标记的开发和 SSR 进化研究提供了有用的信息,具有重要的参考价值.
微衛星或簡單序列重複(SSR)是指以1~6箇堿基為單位的串聯重複序列.SSR 包括完整型和不完整型兩種.不完整型 SSR 是指其序列中存在箇彆不符閤基序的堿基.SSR 作為一種用途廣汎的分子標記,其多態性水平越高則應用價值越大.本研究利用模式植物水稻的兩箇品種(秈稻93-11和粳稻日本晴)和擬南芥的兩箇生態型(Columbia 和 Lansberg)的基因組序列,以及稻瘟菌中245箇 SSR 標記的試驗結果,對 SSR 序列完整性與多態性之間的關繫進行瞭分析.結果錶明,總體上三箇物種都錶現為不完整型 SSR 的多態性比完整型 SSR 低,其中以稻瘟菌最為顯著.水稻和擬南芥錶現齣相似的規律,其 SSR 序列完整性對多態性的影響都是以二覈苷痠重複最為顯著,而五覈苷痠重複序列則基本不受影響;另外,SSR 多態性水平與序列完整程度呈正相關關繫,但輕微的序列不完整一般併不會降低 SSR 的多態性,甚至在擬南芥中還提高多態性.本研究結果對 SSR 標記的開髮和 SSR 進化研究提供瞭有用的信息,具有重要的參攷價值.
미위성혹간단서렬중복(SSR)시지이1~6개감기위단위적천련중복서렬.SSR 포괄완정형화불완정형량충.불완정형 SSR 시지기서렬중존재개별불부합기서적감기.SSR 작위일충용도엄범적분자표기,기다태성수평월고칙응용개치월대.본연구이용모식식물수도적량개품충(선도93-11화갱도일본청)화의남개적량개생태형(Columbia 화 Lansberg)적기인조서렬,이급도온균중245개 SSR 표기적시험결과,대 SSR 서렬완정성여다태성지간적관계진행료분석.결과표명,총체상삼개물충도표현위불완정형 SSR 적다태성비완정형 SSR 저,기중이도온균최위현저.수도화의남개표현출상사적규률,기 SSR 서렬완정성대다태성적영향도시이이핵감산중복최위현저,이오핵감산중복서렬칙기본불수영향;령외,SSR 다태성수평여서렬완정정도정정상관관계,단경미적서렬불완정일반병불회강저 SSR 적다태성,심지재의남개중환제고다태성.본연구결과대 SSR 표기적개발화 SSR 진화연구제공료유용적신식,구유중요적삼고개치.
Microsatellites or simple sequence repeats (SSRs) are tandem repeats of 1 to 6 base pairs. There are two types of SSRs, namely, perfect SSR and imperfect SSR. An imperfect SSR contains one or more bases that do not follow the motif. As a widely utilized molecular marker, the higher polymorphism level of SSR is, the greater ap-plication value of SSR will be. In this study, the relationship between sequence completeness and polymorphism of SSR was investigated by using the genome sequences of two rice varieties (indica rice 93-11 and japonica rice Ni-pponbare) and two ecotypes of Arabidopsis (Columbia and Lansberg) as well as the experimental results of 245 SSR markers in rice blast pathogen Magnaporthe grisea. The results indicated that in general the imperfect SSRs had lower polymorphism levels than that of the perfect SSRs in all the three species, with M. grisea being the most ap-parent. Rice and Arabidopsis exhibited similar results. In both species, the influence of sequence completeness on polymorphism was the most significant for dinucleotide repeats, but little for pentanucleotide repeats; in addition, there is positive correlation between SSR polymorphism and sequence completeness, but slight incompleteness did not reduce the polymorphism level, in contrast, increased the polymorphism in Arabidopsis. The results of this study may provide useful information and valuable reference for the development of SSR markers and the study of SSR evolution.
