基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
GENOMICS AND APPLIED BIOLOGY
2013年
1期
14-21
,共8页
胡太蛟*%武建伟*%潘大仁**%王锋**
鬍太蛟*%武建偉*%潘大仁**%王鋒**
호태교*%무건위*%반대인**%왕봉**
水稻%MAR%转基因水稻%表达与调控
水稻%MAR%轉基因水稻%錶達與調控
수도%MAR%전기인수도%표체여조공
Rice (Oryza sativa L.)%MAR%Transgenic rice%Gene expression and regulation
利用水稻基因组数据库和MAR预测技术,快速筛选和克隆到10条水稻MAR序列.将其分别构建到转基因结构的两侧并整合到水稻中,分析所克隆的序列对转基因表达的功能效应.结果表明:其中的3条序列具有MAR序列的功能,能明显地提高外源转基因的表达水平和表达稳定性,转基因的表达水平平均提高约2~19倍,最高可达28.9倍.
利用水稻基因組數據庫和MAR預測技術,快速篩選和剋隆到10條水稻MAR序列.將其分彆構建到轉基因結構的兩側併整閤到水稻中,分析所剋隆的序列對轉基因錶達的功能效應.結果錶明:其中的3條序列具有MAR序列的功能,能明顯地提高外源轉基因的錶達水平和錶達穩定性,轉基因的錶達水平平均提高約2~19倍,最高可達28.9倍.
이용수도기인조수거고화MAR예측기술,쾌속사선화극륭도10조수도MAR서렬.장기분별구건도전기인결구적량측병정합도수도중,분석소극륭적서렬대전기인표체적공능효응.결과표명:기중적3조서렬구유MAR서렬적공능,능명현지제고외원전기인적표체수평화표체은정성,전기인적표체수평평균제고약2~19배,최고가체28.9배.
Ten MAR sequences of rice were selected to be cloned quickly by using the rice genome database and the prediction of MAR. And their effects on the expression of the transgene were analyzed in transgenic rice by integrat-ing the MAR sequences to both sides of the transgene. This research showed that three of the ten MAR sequences could improve the expression level and stability of the transgene obviously. The expression level of the transgene was improved about from2 to 19 times average compared to the control and the maximumwas up to 28.9 times.
