CAJ | 학술논문

GH15菌株在高NH4+浓度下能够积累过量的谷氨酰胺,并且随着NH4+浓度的增加,谷氨酰胺的产量逐渐增加,但谷氨酸的产量却逐渐降低,这表明该菌株在不同NH4+浓度下其高胞内的氮代谢及调控行为有很大的改变.利用抑制消减杂交技术分别构建了GH15菌株在高NH4+浓度下和低NH4+浓度下的基因表达抑制消减文库.通过对文库的测序得到了35个差异表达的基因.这些差异基因主要是细胞生长相关基因、能量代谢基因以及氨基酸合成代谢相关的基因,我们认为正是这些基因差异导致该菌株在高NH4+下高产谷氨酰胺.
GH15균주재고NH4+농도하능구적루과량적곡안선알,병차수착NH4+농도적증가,곡안선알적산량축점증가,단곡안산적산량각축점강저,저표명해균주재불동NH4+농도하기고포내적담대사급조공행위유흔대적개변.이용억제소감잡교기술분별구건료GH15균주재고NH4+농도하화저NH4+농도하적기인표체억제소감문고.통과대문고적측서득도료35개차이표체적기인.저사차이기인주요시세포생장상관기인、능량대사기인이급안기산합성대사상관적기인,아문인위정시저사기인차이도치해균주재고NH4+하고산곡안선알.
Arthrobacter globiformis strain GH15 can excessively accumulate glutamine in high NH4+concentra-tion, and glutamine yield can gradually increase in a certain range with the increase of NH4+, while the yield of glutamate can reduce accordingly, which indicates that nitrogen metabolism and regulation in the cell of GH15 should have dramatic changes in high NH4+ concentrations. The gene expression subtractive suppression hy-bridization library in the different NH4+concentration was constructed in this research, 35 differentially expressed genes were obtained and sequenced, which focused on the genes that mainly related to cell growth, energy metabolism and metabolism of amino acids. We believed that it is the differences of these genes lead to the strain produces high glutamine yield in high NH4+concentration.