CAJ | 학술논문

从平菇废弃培养料中分离获得一株细菌B3,通过对其进行形态学、培养特征比较研究和16S rDNA序列分析,鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens).在与常见的三种食用菌污染真菌脉孢菌(Neur ospora sp.)、木霉(Trichoderma sp.)及鬼伞(Coprinus sp.)分别进行对峙培养时,都有明显的抑菌作用,形成的抑菌带分别为0.38 cm、0.57 cm及0.56 cm,而与平菇(Pleurotus ostreatus)、鸡腿菇(Coprinus comatus)及草菇(Volvariella volvacea)分别进行对峙培养时,除对草菇有明显的抑制作用外,对平菇和鸡腿菇的抑制作用很小.在混接B3菌株麸皮菌剂栽培料培养基上,B3菌株对三种污染真菌的抑制作用都很明显;可抑制木霉分生孢子形成、脉孢菌分生孢子萌发及鬼伞菌丝扩展;但不抑制平菇菌丝的生长.
종평고폐기배양료중분리획득일주세균B3,통과대기진행형태학、배양특정비교연구화16S rDNA서렬분석,감정위해정분아포간균(Bacillus amyloliquefaciens).재여상견적삼충식용균오염진균맥포균(Neur ospora sp.)、목매(Trichoderma sp.)급귀산(Coprinus sp.)분별진행대치배양시,도유명현적억균작용,형성적억균대분별위0.38 cm、0.57 cm급0.56 cm,이여평고(Pleurotus ostreatus)、계퇴고(Coprinus comatus)급초고(Volvariella volvacea)분별진행대치배양시,제대초고유명현적억제작용외,대평고화계퇴고적억제작용흔소.재혼접B3균주부피균제재배료배양기상,B3균주대삼충오염진균적억제작용도흔명현;가억제목매분생포자형성、맥포균분생포자맹발급귀산균사확전;단불억제평고균사적생장.
The bacterial strain B3 was isolated from spend oyster mushroom substrate, it was identified as Bacill us amyloliquefaciens based on morphological and physiological characteristics and 16S rDNA analysis. The strain B3 showed high antagonistic activity against the common mushroom contaminative fungi such as Neurospora sp., Trichoderma sp. and Coprinus sp.. In dual plate assay, the inhibition zone was 0.38 cm, 0.57 cm and 0.56 cm, re-spectively. The strain B3 was also cultured in dual plate with Pleurotus ostreatus, Coprinus comatus and Volvariella volvacea, respectively. The strain B3 showed very low inhibition to Pleurotus ostreatus and Coprinus comatus, but it had significant inhibition to Volvariella volvacea. Strain B3 showed stronger inhibition to Neurospora sp., Trichod ermasp. and Coprinus sp. in the cottonseed husk substrate when inoculated with oyster mushroom. It could inhibit the conidia formation of Trichoderma sp., the spore germination of Neurospora sp. and the hyphal extension of Coprinus sp., but showed lower inhibition to oyster mushroom.